Home | Community | Message Board


Original Seeds Store - Cannabis Seeds
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: Agar, Paul Stamets

Jump to first unread post. Pages: 1
Offlinecupid
Trust noone

Registered: 03/23/03
Posts: 54
Last seen: 14 years, 6 months
Cold Storage for strains
    #1424355 - 04/02/03 10:31 PM (15 years, 20 days ago)

According to a source SWIM read somewhere test tubes could be used to house mycelium cultures in cold storage is this true and if so, SWIM has noticed a +/- 10% variance in the fridges temperatures is this within acceptable parameters ? Also a store ending in MART sells small fridges for around 100 bucks do you think these would have less of a temperature variance ? also what is the maximum storage time for a syringe filled with a mycelium broth and should this also be placed in cool storage ?


--------------------
Final last words... What's this button do ?


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineamyloid
Stranger
Registered: 03/08/03
Posts: 980
Last seen: 4 years, 2 months
Re: Cold Storage for strains [Re: cupid]
    #1424600 - 04/03/03 12:01 AM (15 years, 20 days ago)

TMC reads in reference to sterile agar slants:


Inncubate for one week at 75? F... Once the mycelia has covered a major portion of the agar's surface and appears to be free of contamination, stor at 35-40? F... At these temperatures, them metabolic activity of most mycelia is lowered to a level where growth and nutrient absorbtion virtually stops. Ideally one should check the vitality of stored culturesevery six months by removing fragments of mycelium and innoculating more perti dishes. Once mycellium has colonized two-thrids of the media dish, select for strandy growth (Rhizomorphism) and reinoculate more slants...


--------------------
"A human being is part of a whole, called by us the Universe, a part limited in time and space. He experiences himself, his thoughts and feelings, as something separated from the rest--a kind of optical delusion of his consciousness. This delusion is a kind of prison for us, restricting us to our personal desires and to affection for a few persons nearest us. Our task must be to free ourselves from this prison by widening our circles of compassion to embrace all living creatures and the whole of nature in its beauty."
-Al Einstein


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinezeronio
Stranger
Male

Registered: 10/16/01
Posts: 2,349
Loc: Slovenia
Last seen: 1 year, 7 months
Re: Cold Storage for strains [Re: cupid]
    #1425537 - 04/03/03 08:45 AM (15 years, 19 days ago)

Take a look at this post!!!


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: Agar, Paul Stamets

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* Single Strain (spore) Isolation and the Benefits.. TrippinRhino 5,340 18 07/24/01 02:02 AM
by Anno
* Storage Bruiser 814 3 03/27/03 04:24 AM
by Bruiser
* Re: Need Advice, storage of agar plates TriGuy 2,275 6 12/20/99 01:58 AM
by Ryche Hawk
* Spawn storage question altarego 497 1 01/04/02 12:39 PM
by Anno
* incubation temperature for p. cubensis adroit_synth 4,099 12 01/24/04 09:27 PM
by adroit_synth
* Optimal Temperatures for Secondary Metabolites Humidity 3,177 10 09/14/01 01:59 PM
by gray1
* Secondary Metabolism: Control by Temperature Humidity 2,412 8 06/21/01 11:37 AM
by Humidity
* MAJOR BREAKTHROUGH - FASTER PINNING VIA TEMPERATURE CYCLING
( 1 2 3 all )
Hannibal88 12,348 40 07/05/13 05:22 PM
by RogerRabbit

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, bodhisatta
880 topic views. 0 members, 5 guests and 2 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Everything Mushrooms
Please support our sponsors.

Copyright 1997-2018 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.005 seconds on 19 queries.