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OfflineGreenBlanket
Mushroom Apprentice
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Registered: 11/15/10
Posts: 52
Loc: Washington
Last seen: 3 years, 2 months
Re: GLC syringe question, multiple transfers..... [Re: Doc_T]
    #14360332 - 04/27/11 09:59 AM (12 years, 9 months ago)

My hands were in rubber gloves in my SAB I had wiped everything down with alcohol from a sponge. I did my best to keep the lids on as much as I could and used the feed syringe without the needle directly from the inside of the jars. It picked up a little bit of wbs that wouldn't have came through the needle normally. The water looks amazing though, so I was thinking as long as I knock up my jars this week the wbs that came with shouldn't be a problem, the transfer would be.Thanks again lol I just felt like it was super contaminant prone so I thought I would ask before inoculating fifty jars!

GB


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Offlinenushie
Funguy
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Registered: 06/03/10
Posts: 72
Last seen: 1 year, 1 month
Re: GLC syringe question, multiple transfers..... [Re: GreenBlanket]
    #14361298 - 04/27/11 02:04 PM (12 years, 9 months ago)

Doc_T was being a smart ass GB. HAHa,  I would try a sample jar with your syringe,  I am about to do the same thing, making 10 syringes I am going to use the last syringe I make and do a "sample jar"  within 2 weeks you should know if you were successful in creating a clean LC.  Let me know how it turns out.  I will be attempting this ass soon as my 60 ml syringe gets here.  Also I finally went out today and bought some RTV silicone,  it was only $5.05 at Autozone.  I also went and got some Tyvek ( I had been using poly).  So at least my sterilized water jar will have a self healing injection port and tyvek filter.  I am going to do this all in a Glove box.  The spawn jar has a poly filter still.  Do you guys think I should just shoot up/extract the myc water through the poly or do you think I should make 2 lids with the self healing injection port, PC both and just try and quickly swap lids on the spawn jar?.  Thanks a bunch for all the help!

-Nushie


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