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i GrOw StUFF
Stranger

Registered: 01/14/11
Posts: 1,739
Loc: Space
Last seen: 9 years, 5 months
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GLC syringe question, multiple transfers.....
#14243525 - 04/05/11 06:37 PM (12 years, 10 months ago) |
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I did a GLC tek and was successful. Syringes are good and tested. Only one left. Ive had it sitting at room temperature for about 4 weeks. Can I use this syringe to inoculate another grain jar that would later be used for more GLC syringes?....Ive been reading about G2G transfers but not sure if this fits in the same category. I didn't mean to keep it out for weeks, but forgot about the little bugger....Any opinions or input here?
-------------------- The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: GLC syringe question, multiple transfers..... [Re: i GrOw StUFF]
#14243549 - 04/05/11 06:40 PM (12 years, 10 months ago) |
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Go ahead and refrigerate it now. Instead of making more GLC, expand the grain with a level of g2g. So a few drops of your master syringe into a grain jar becomes 5-10 quarts of spawn.
-------------------- You make it all possible. Doesn't it feel good?
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i GrOw StUFF
Stranger

Registered: 01/14/11
Posts: 1,739
Loc: Space
Last seen: 9 years, 5 months
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Re: GLC syringe question, multiple transfers..... [Re: Doc_T]
#14243748 - 04/05/11 07:10 PM (12 years, 10 months ago) |
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ok doc thanks for the reply. Have to get some agar too. I have shitload of prints and want to clean some things up...
-------------------- The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.
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M11
White Thumb


Registered: 06/14/09
Posts: 1,840
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Re: GLC syringe question, multiple transfers..... [Re: Doc_T]
#14243969 - 04/05/11 07:49 PM (12 years, 10 months ago) |
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Quote:
Doc_T said: Go ahead and refrigerate it now. Instead of making more GLC, expand the grain with a level of g2g. So a few drops of your master syringe into a grain jar becomes 5-10 quarts of spawn.
I am confused by this. Please explain.
-------------------- Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter. Just pretend it's christmas. -RR Those mushrooms are fine. Your friend is a pussy. -RR Outdoor Patch
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: GLC syringe question, multiple transfers..... [Re: M11]
#14243993 - 04/05/11 07:53 PM (12 years, 10 months ago) |
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1CC GLC > 1L Rye
1L Rye G2G > 10L Rye
10L Rye G2G > 100L Rye
1cc of grain LC can be turned into 100L of grain spawn in no time.
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M11
White Thumb


Registered: 06/14/09
Posts: 1,840
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Re: GLC syringe question, multiple transfers..... [Re: EvilMushroom666]
#14244027 - 04/05/11 07:57 PM (12 years, 10 months ago) |
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How is this better than just making another LC? LCs are faster anyway.
-------------------- Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter. Just pretend it's christmas. -RR Those mushrooms are fine. Your friend is a pussy. -RR Outdoor Patch
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: GLC syringe question, multiple transfers..... [Re: M11]
#14244052 - 04/05/11 08:02 PM (12 years, 10 months ago) |
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To each there own,
I prefer G2G to expand my edible spawn, some prefer LC. Whatever works for you.
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M11
White Thumb


Registered: 06/14/09
Posts: 1,840
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Re: GLC syringe question, multiple transfers..... [Re: EvilMushroom666]
#14244094 - 04/05/11 08:10 PM (12 years, 10 months ago) |
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How much grain do you usually transfer into another grain jar? Do you mix the grain around after inoculating the fresh grain?
-------------------- Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter. Just pretend it's christmas. -RR Those mushrooms are fine. Your friend is a pussy. -RR Outdoor Patch
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: GLC syringe question, multiple transfers..... [Re: M11]
#14244198 - 04/05/11 08:27 PM (12 years, 10 months ago) |
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It all depends, the more grain you use the faster your donor jars will colonize obviously.
On a recent shiitake spawn run I went from 1L of wheat berries over to 10 donor jars. I shake the jars after the G2G, and once again at around 30-40%. Jars usually finish up between 8-14 days depending upon the amount of spawn used.
I also find that half pints are good G2G starters and you can go from 1 Pint of colonized grain > 4-5L's of donor grain.
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: GLC syringe question, multiple transfers..... [Re: M11]
#14246098 - 04/06/11 04:31 AM (12 years, 10 months ago) |
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Quote:
M11 said: How is this better than just making another LC? LCs are faster anyway.
Faster is not necessarily better. And starting LC from GLC is better than spores, but still sketchy. GLC->grain master->g2g is the safest and fastest way to get Too Much Spawn.
-------------------- You make it all possible. Doesn't it feel good?
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Morelman
LC Master



Registered: 05/17/06
Posts: 2,625
Loc: Hiding under an Elm Tree
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Re: GLC syringe question, multiple transfers..... *DELETED* [Re: Doc_T]
#14248989 - 04/06/11 06:12 PM (12 years, 10 months ago) |
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Post deleted by MorelmanReason for deletion: Never again...
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: GLC syringe question, multiple transfers..... [Re: Morelman]
#14249112 - 04/06/11 06:35 PM (12 years, 10 months ago) |
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One drawback is having to test your LC`s before using it for big projects. You can tell if a grain jar has any contaminates before you G2G out.
You only lose 1-3 days testing your LC(If you use agar) but that is still a few days slower then visually inspecting your G2G jars.
NOTE: I am talking about sugar based LC`s here, not GLC.
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Morelman
LC Master



Registered: 05/17/06
Posts: 2,625
Loc: Hiding under an Elm Tree
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Re: GLC syringe question, multiple transfers..... *DELETED* [Re: EvilMushroom666]
#14249154 - 04/06/11 06:46 PM (12 years, 10 months ago) |
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Post deleted by MorelmanReason for deletion: Never again...
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: GLC syringe question, multiple transfers..... [Re: Morelman]
#14249176 - 04/06/11 06:49 PM (12 years, 10 months ago) |
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See above, I am not talking about GLC, but sugar based LC`s.
As far as I am concerned in the G2G VS GLC debate, take your pick they both have the same results.
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Morelman
LC Master



Registered: 05/17/06
Posts: 2,625
Loc: Hiding under an Elm Tree
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Re: GLC syringe question, multiple transfers..... *DELETED* [Re: EvilMushroom666]
#14249216 - 04/06/11 06:56 PM (12 years, 10 months ago) |
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Post deleted by MorelmanReason for deletion: Never again...
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nushie
Funguy



Registered: 06/03/10
Posts: 72
Last seen: 1 year, 2 months
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Re: GLC syringe question, multiple transfers..... [Re: Morelman]
#14358316 - 04/26/11 10:27 PM (12 years, 10 months ago) |
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Those using GLC are getting it using Agars tek im assuming. At the end of the tek he just states something like now you have 500ml of myc water so you can fill Ten 10cc syringes of myc water. The question I have is can this be done immediately or does the myc have to sit in the jar for a few days to recover? Also what is the recommended shelf life on these Syringes once made?
Thanks,
-Nushie
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: GLC syringe question, multiple transfers..... [Re: nushie]
#14358368 - 04/26/11 10:32 PM (12 years, 10 months ago) |
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Ok to use immediately, store in the fridge for months.
-------------------- You make it all possible. Doesn't it feel good?
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: GLC syringe question, multiple transfers..... [Re: nushie]
#14358396 - 04/26/11 10:35 PM (12 years, 10 months ago) |
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You can draw out your grain LC syringes immediately after creation.
As the GLC sits in the syringe for a few days you will notice the mycelium will recover and start to grow back together into clumps. Be sure to give it a good shake before using to break up the mycelium and create more inoculation points.
In my experience a grain LC syringe will stay viable for 5-6 months if stored under refrigeration.
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GreenBlanket
Mushroom Apprentice



Registered: 11/15/10
Posts: 52
Loc: Washington
Last seen: 3 years, 3 months
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Re: GLC syringe question, multiple transfers..... [Re: EvilMushroom666]
#14359395 - 04/27/11 02:42 AM (12 years, 10 months ago) |
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Hey guys so I went to make my glc today from my two wbs jars and came to realize that the feed store which supplied my 60ml syringe gave me needles that don't fit! So I'm sitting there all SABed up and said fuck it and pulled the lid off of one of my grain jars and sterile water jars. Then made my GLC with the lids off almost like you would G2G. I understand this is MUCH more risky with contams but was just curious if there is pretty much no chance this will be contam free? In this ghetto process a piece of millet or two came through will this be a problem if I use the lc this week? (I assume much after that those will for sure cause contamination). Either way tomorrow I will hunt down the needle I need and properly make my other glc!
GB
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: GLC syringe question, multiple transfers..... [Re: GreenBlanket]
#14359682 - 04/27/11 06:08 AM (12 years, 10 months ago) |
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How long did you wash your hands for before opening the jar?
-------------------- You make it all possible. Doesn't it feel good?
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