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Offlinek00laid
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Registered: 05/03/10
Posts: 19,636
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Re: 1 Pint BRF Cakes [Re: FrequentFryer]
    #14218215 - 04/01/11 12:10 AM (12 years, 10 months ago)

Quote:

FrequentFryer said:
B+ strain and i came to the concusion that its a slow colonizer. I was proved wrong.




spores dude, spores...


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OfflineFrequentFryer
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Re: 1 Pint BRF Cakes [Re: k00laid]
    #14218223 - 04/01/11 12:11 AM (12 years, 10 months ago)

Quote:

k00laid said:
Quote:

FrequentFryer said:
B+ strain and i came to the concusion that its a slow colonizer. I was proved wrong.




spores dude, spores...



Check out what i just posted.. What do you think? :l

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Offlinek00laid
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Re: 1 Pint BRF Cakes [Re: FrequentFryer]
    #14218231 - 04/01/11 12:13 AM (12 years, 10 months ago)

unflamed needle is a reeeeal bad mistake.

id start more jars if you are counting on these producing


i also flame needle between each jar.


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OfflineFrequentFryer
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Re: 1 Pint BRF Cakes [Re: k00laid]
    #14218241 - 04/01/11 12:16 AM (12 years, 10 months ago)

Quote:

k00laid said:
unflamed needle is a reeeeal bad mistake.

id start more jars if you are counting on these producing


i also flame needle between each jar.



Yeah thats what i usually alwayss do. Last time i used it i flamed/wiped it with alcohol and capped it. (Im pretty sure its a 100 percent seal)
It's whatever.. I might get lucky and get away with it. Gotta quit smoking so much weed..:rasta:

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Offlinek00laid
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Re: 1 Pint BRF Cakes [Re: FrequentFryer]
    #14218247 - 04/01/11 12:17 AM (12 years, 10 months ago)

xDD

well alcohol sanitizes and does not sterilize.

nothing gets cleaner than red hot metal. no need for the alcohol.


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OfflineFrequentFryer
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Re: 1 Pint BRF Cakes [Re: k00laid]
    #14218255 - 04/01/11 12:21 AM (12 years, 10 months ago)

Quote:

k00laid said:
xDD

well alcohol sanitizes and does not sterilize.

nothing gets cleaner than red hot metal. no need for the alcohol.



Yeah foshoo, im just a clean freak and like to get that soot and whatnot off the needle..
Well shit. lol
I'll keep you guys updated on this jar.. least i know i'll never do this again hahahha. Once it gets some growth goin i'll be a little reassured..

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Offlinek00laid
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Re: 1 Pint BRF Cakes [Re: FrequentFryer]
    #14218262 - 04/01/11 12:23 AM (12 years, 10 months ago)

that soot has less of a chance of bacteria or a mold spore being in it than your alcohol pad does.


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Invisiblebiologys
Mycologist in Trainning
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Re: 1 Pint BRF Cakes [Re: k00laid]
    #14218274 - 04/01/11 12:25 AM (12 years, 10 months ago)

if you use denatured alcohol, in an alcohol lamp you have zero soot on your needle


a few times i've run out and used paint thinner, now that produces some black shit let me tell ya lol, but it doesn't effect anything

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OfflineFrequentFryer
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Re: 1 Pint BRF Cakes [Re: biologys]
    #14218300 - 04/01/11 12:31 AM (12 years, 10 months ago)

Yeah thanks for all these tips, i would have thought an alcohol pad was 100% sterile..
But yeah, i usually flip a cup over and put iso in the concave in the bottom of the cup.. leaves little to no soot.
What do you guys think my chances are of a contam without sterilization of the needle? Mind you it never touched anything unsterile..
Still. i don't think my chances are to good lol

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Invisiblebiologys
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Re: 1 Pint BRF Cakes [Re: FrequentFryer]
    #14218316 - 04/01/11 12:34 AM (12 years, 10 months ago)

its possible, when i first started I nocc'd up like 15 jars with out flaming between the jars, and only 2 contam'd.

but keep in mind, each time that needle is exposed to open air, chances of mold spores getting on it are increased...as well if 1 jar is contaminated and didn't get properly sterilized, you're spreading that to your other jars..

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OfflineFrequentFryer
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Re: 1 Pint BRF Cakes [Re: biologys]
    #14218366 - 04/01/11 12:42 AM (12 years, 10 months ago)

Quote:

biologys said:
its possible, when i first started I nocc'd up like 15 jars with out flaming between the jars, and only 2 contam'd.

but keep in mind, each time that needle is exposed to open air, chances of mold spores getting on it are increased...as well if 1 jar is contaminated and didn't get properly sterilized, you're spreading that to your other jars..



Yeah forsure i'm trying to using one strain at a time. Both times before i only knocked up 2 jars on 2 seperate occasions. Both times alcohol/flame sterilization where used. And yeah it wasn't really exposed to air for to long either, it had a snap seal cap on it.
It doesn't really bother me that i wasted spore solution, its the verm and brf. I only found one store in the whole town that sells verm and i bought the last bag lol.
Ohh well though, i have high hopes. And when it comes down to it, i only lost what a dollar worth of materials? hah.
Thanks guys. Really diggin this community so far, lots of help within an hour or so. Also i like getting your guy's detailed specific answers on shit.. :thumbup:

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OfflineFrequentFryer
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Re: 1 Pint BRF Cakes [Re: FrequentFryer]
    #14220949 - 04/01/11 01:28 PM (12 years, 10 months ago)

So, what do you guys think about my cakes? :mushroom2:

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OfflineFrequentFryer
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Re: 1 Pint BRF Cakes [Re: FrequentFryer]
    #14242783 - 04/05/11 04:32 PM (12 years, 10 months ago)

Alright, well my furthest jar is at 95% with verryyy dense mycelium growth. The second furthest half pint is at about 65-70%, and the pinter shows strong growth in all 4 inoculation points. I have no doubt that the pint jar will go smoothly as long as it doesn't get contaminated. Question. If it was contamed when I innoc'd should I have already seen the contam? Or could it show at any time?
Also, gathered some very nicee leached horse nuggets, stay tuned :smile:

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OfflineSomeGuy
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Re: 1 Pint BRF Cakes [Re: FrequentFryer]
    #14243008 - 04/05/11 05:06 PM (12 years, 10 months ago)

I don't think I've ever got an infection after all 4 spots touched. Take this advice. Don't tilt flip or shake them. Keeping them upright is the only way to go:awesomenod:thats the no. 1 screw-up I see peaple fail because of

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OfflineFrequentFryer
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Re: 1 Pint BRF Cakes [Re: SomeGuy]
    #14243061 - 04/05/11 05:17 PM (12 years, 10 months ago)

Yeah, shakings a big no-no but flipping is possible. I flipped mine with no adverse effects maybe stunted it for a day but its back on track and almost done. I think ill go ahead and birth it Friday.

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OfflineMacaman
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Re: 1 Pint BRF Cakes [Re: FrequentFryer]
    #21681616 - 05/15/15 02:31 AM (8 years, 9 months ago)

When you knock up your jar's are you doing it in a glove box or free air?

  I've knocked up a total of 25 jars with syringes and did 7 g2g transfers using the oven method and I've only had issues with 5 jars total. And they were all from the same strain. ( thinking I got a contaminated strain, but I digress ).


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Offlinehomerj
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Registered: 11/25/15
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Re: 1 Pint BRF Cakes [Re: FrequentFryer]
    #22659141 - 12/15/15 03:56 PM (8 years, 2 months ago)

brf jars colonize fairly slowly, your best bet would be to do more 1/2 pint jars and stack the cakes, as opposed to doing full pint jars. less risk of contamination.

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InvisibleMajorDick
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Re: 1 Pint BRF Cakes [Re: homerj]
    #22661642 - 12/16/15 01:39 AM (8 years, 2 months ago)

Quote:

homerj said:
brf jars colonize fairly slowly, your best bet would be to do more 1/2 pint jars and stack the cakes, as opposed to doing full pint jars. less risk of contamination.




I'm sure he's figured it out by meow.

Come back to page one, man!

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