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OfflineProbationBlows
Stranger

Registered: 03/19/11
Posts: 51
Last seen: 12 years, 5 months
Cloning... LC or agar for beginner
    #14241735 - 04/05/11 12:43 PM (12 years, 9 months ago)

Hey guys, i was just wondering if it is recommended that a beginner use agar to clone or anything of that sort... i plan on using a few MS syringes first but i was wondering if its recommended that a beginner clone a mushroom... i wanna be able to have a great strain that i can keep on using with consistent results... any info would help  thanks


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Offlinemickle207
Stranger Shrooms

Registered: 09/03/10
Posts: 164
Last seen: 4 years, 3 months
Re: Cloning... LC or agar for beginner [Re: ProbationBlows]
    #14241784 - 04/05/11 12:59 PM (12 years, 9 months ago)

Try this: http://www.shroomery.org/forums/showflat.php/Number/5874305#5874305

Its really simple and it made perfect LC for me. I knocked up a couple of bags already and they are colonising well, I will tell u the results of the fruits.


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OfflineLarrythescaryrex
teardrop on the fire
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Registered: 07/19/00
Posts: 11,004
Loc: further down the spiral
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Re: Cloning... LC or agar for beginner [Re: mickle207]
    #14241835 - 04/05/11 01:08 PM (12 years, 9 months ago)

make a still air box and do agar. its easy. Look for my first time agar use grow log in the grow log area.


--------------------
RIP Acidic_Sloth

Sunset_Mission said:
"larry the scary rex
verily scary when thoroughly vexed
invoke the shadows and dust, cast a hex
mercifully massacring memories masterfully
relocate from Ur to 8th density and become a cosmic bully
mulder and scully couldn't decipher his glyphs
invoke the shadows and dust, smoke infernal spliffs"
April 24th 2011


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OfflineLarrythescaryrex
teardrop on the fire
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Registered: 07/19/00
Posts: 11,004
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Re: Cloning... LC or agar for beginner [Re: Larrythescaryrex]
    #14241840 - 04/05/11 01:10 PM (12 years, 9 months ago)

Let me clarify. People think agar is like a magical super thing. But after working with it I have realized that it is something anyone can do and people should get into it sooner than they do. Plus you can work from prints more easily and decent prints are easier to get than good syringes.


--------------------
RIP Acidic_Sloth

Sunset_Mission said:
"larry the scary rex
verily scary when thoroughly vexed
invoke the shadows and dust, cast a hex
mercifully massacring memories masterfully
relocate from Ur to 8th density and become a cosmic bully
mulder and scully couldn't decipher his glyphs
invoke the shadows and dust, smoke infernal spliffs"
April 24th 2011


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OfflineDamion5050
Mush Doctor
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Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation ! Flag
Last seen: 3 years, 5 days
Trusted Cultivator
Re: Cloning... LC or agar for beginner [Re: Larrythescaryrex]
    #14241866 - 04/05/11 01:16 PM (12 years, 9 months ago)

Quote:

Larrythescaryrex said:
Let me clarify. People think agar is like a magical super thing. But after working with it I have realized that it is something anyone can do and people should get into it sooner than they do. Plus you can work from prints more easily and decent prints are easier to get than good syringes.





What he said lmao..  Don't be scared to start using agar it really isn't that hard to do, all you need is some agar powder and some petri's and if you cant get petri's you can actually use 1/2 pint or pint jars instead..

Agar makes it really easy to take clean samples and make sure they are clean samples, the thing with cloning to an LC is that you never know if your sample is truly clean and contam free until you shoot up some jars..

But if you use agar you will know within a day or 2 if you have a clean sample, and if it is contam you can transfer away from the contam to get healthy mycelium and then either do agar to grain or agar to LC..

IMHO agar is the best way to go even if you are a noob, it isn't hard at all, once you do it you will be punching yourself in the face and asking the question why didn't I get into agar ages ago.


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InvisibleEvilMushroom666
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Posts: 10,289
Loc: Canada Flag
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Re: Cloning... LC or agar for beginner [Re: Damion5050]
    #14242139 - 04/05/11 02:10 PM (12 years, 9 months ago)

Here are a few articles I wrote up that may help you out:

Agar Setup(Costs and Materials)
https://www.shroomery.org/forums/showflat.php/Number/12675513

Pouring Malt Extract Agar
https://www.shroomery.org/forums/showflat.php/Number/12748848

These two articles chronical my start with pouring agar inside of a
glove box. I have built a flow hood since, but doing agar work is 100%
doable inside a glove box.


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Invisiblemaiko maiko
I can eat 50 eggs

Registered: 02/26/10
Posts: 332
Re: Cloning... LC or agar for beginner [Re: EvilMushroom666]
    #14242183 - 04/05/11 02:19 PM (12 years, 9 months ago)

+1 for agar. it's really very simple. i simmer and pour it into wide mouth 1/2 pints with a filter paper over a few holes for GE and pressure cook for 15 minutes. once it cools it should be fine and any moisture should be reabsorbed within a day or two.


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OfflineMycowlogist
Freethinker
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Registered: 06/29/08
Posts: 534
Loc: in a galaxy far far away....
Last seen: 10 years, 27 days
Re: Cloning... LC or agar for beginner [Re: EvilMushroom666]
    #14242195 - 04/05/11 02:21 PM (12 years, 9 months ago)

I think it is whatever works best for you.  Try whatever you think is easier, if you have problems learn from your mistakes and try the other method.  Both methods are successful, and have their pro and cons.

I tried LC and had 1 viable 30 oz LC in my first try.  I agree you don't know what you have until you test it, simple enough to do though.  I like LC, but will be moving to agar to start working with isolates.


--------------------
"And in the end, it's not the years in your life that count. It's the life in your years."  Honest Abe


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Invisiblemaiko maiko
I can eat 50 eggs

Registered: 02/26/10
Posts: 332
Re: Cloning... LC or agar for beginner [Re: Mycowlogist]
    #14242212 - 04/05/11 02:25 PM (12 years, 9 months ago)

i like transferring agar wedges to LC(agar broth) in a still air box. LC colonizes much faster than an agar transfer to grain.


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Offlinelil_demented
Loner will lone
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Registered: 09/11/06
Posts: 6,146
Last seen: 1 month, 9 days
Re: Cloning... LC or agar for beginner [Re: Mycowlogist]
    #14242221 - 04/05/11 02:28 PM (12 years, 9 months ago)

:popcorn:
Good info I'll wanna find later.


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Offlineshroomie_glen
RedHotPussyLiquor
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Registered: 03/01/06
Posts: 4,296
Loc: Narf Carolina Flag
Last seen: 11 years, 3 months
Re: Cloning... LC or agar for beginner [Re: maiko maiko]
    #14242223 - 04/05/11 02:29 PM (12 years, 9 months ago)

Agar, specifically PDA is the shizzznit.  i love how you can print in the field and take it indoors and cult. from there.
Cant do that with a Lc.


--------------------

No. No, man. Shit, no man.  I believe you'd get your ass kicked sayin' somethin' like that man.


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