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OfflineOneU

Registered: 03/19/11
Posts: 763
Last seen: 12 years, 13 days
Re: I need help with sterilization. Will this work?? [Re: cyantific]
    #14238631 - 04/04/11 09:14 PM (12 years, 10 months ago)

no the vendor told me on friday and on saturday it will be here on saturday probably and on monday probably.

but here are some pics of the jars. are they ruined? they have some sort of condensation inside (i got the moisture and everything right):





thanks a lot

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OfflineOneU

Registered: 03/19/11
Posts: 763
Last seen: 12 years, 13 days
Re: I need help with sterilization. Will this work?? [Re: OneU]
    #14238652 - 04/04/11 09:16 PM (12 years, 10 months ago)

and that line is an estimate of where the water rose during boil. it started to boil then i turned it down to medium. i did the 2-1-1 verm/brf/water followed pf tek exactly, did the wipe down of moisture, stacked lids, but somehow the water wasn't measured completely off of the jars (i think it was 1/3 submerged or even 1/2...). i read on some threads that this is ok but others that it's not.

any and all help is deeply appreciated. thank you again. <33

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Invisiblehamloaf
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Registered: 12/23/09
Posts: 20,575
Loc: Oklahoma. Flag
Trusted Cultivator
Re: I need help with sterilization. Will this work?? [Re: OneU]
    #14238735 - 04/04/11 09:28 PM (12 years, 10 months ago)

Boiling water can come up from the bottom of the sterilizer during the sterilization run and get into the jars between the threads and lids ruining the hard worked for moisture content of the substrate during the sterilization cylcle which is why it's prescribed to not submerge, at all, those little half-pint jars during sterilization.  The fact that they were is ok so long as water didn't find it's way into your jars.  Also, steam is the best at transferring heat into items that are desired to be sterilized.


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How I Get Stuff done. - My Reference Guide. - My Grows.

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OfflineOneU

Registered: 03/19/11
Posts: 763
Last seen: 12 years, 13 days
Re: I need help with sterilization. Will this work?? [Re: OneU]
    #14239098 - 04/04/11 10:26 PM (12 years, 10 months ago)

i see. so are the jars ok for inoculation or should i redo the sub?

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Invisiblebiologys
Mycologist in Trainning
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Registered: 12/21/09
Posts: 4,622
Re: I need help with sterilization. Will this work?? [Re: OneU]
    #14239179 - 04/04/11 10:42 PM (12 years, 10 months ago)

those jars look fine to me, nocc em up and set and forget them for about 7-10 days, then come back and check for growth.

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Invisiblehamloaf
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Registered: 12/23/09
Posts: 20,575
Loc: Oklahoma. Flag
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Re: I need help with sterilization. Will this work?? [Re: OneU]
    #14239191 - 04/04/11 10:44 PM (12 years, 10 months ago)

They look ok to me too.  Just be sure you inspect each of your jars to see if any heavy moisture pooling is present at the bottom of the jars before considering inoculating or starting over.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.

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OfflineOneU

Registered: 03/19/11
Posts: 763
Last seen: 12 years, 13 days
Re: I need help with sterilization. Will this work?? [Re: hamloaf]
    #14239233 - 04/04/11 10:53 PM (12 years, 10 months ago)

alright you guys. i really appreciate your help. this is a very kind and generous community. very informative and knowledgeable and very present. :awesome2:

thanks again. i will do so.

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Offlineshroomie_glen
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Registered: 03/01/06 Happy 18th Shroomiversary!
Posts: 4,296
Loc: Narf Carolina Flag
Last seen: 11 years, 4 months
Re: I need help with sterilization. Will this work?? [Re: OneU]
    #14239355 - 04/04/11 11:13 PM (12 years, 10 months ago)

Time is your greatest ally my newfound compatriate.


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No. No, man. Shit, no man.  I believe you'd get your ass kicked sayin' somethin' like that man.

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