Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinequebus
Stranger

Registered: 05/01/10
Posts: 678
Last seen: 2 months, 23 days
Inoculate then shake??
    #14238317 - 04/04/11 08:17 PM (12 years, 9 months ago)

I inoculated 5 quart jars today (rye grain) with LC (2 qts) and spore syringe (3 qts).  Was I supposed to shake the jars after inoculation?

If yes - can I do it now?

Thanks.


Extras: Filter Print Post Top
OfflineSTR80180
Stranger

Registered: 12/13/10
Posts: 142
Last seen: 3 years, 8 months
Re: Inoculate then shake?? [Re: quebus]
    #14238340 - 04/04/11 08:20 PM (12 years, 9 months ago)

I've tried both ways and didn't make much difference


Extras: Filter Print Post Top
OfflineTrippy_Smurf
Sketchy Mother Fucker
Male User Gallery


Folding@home Statistics
Registered: 02/14/11
Posts: 2,349
Loc: Smurf Villiage Flag
Last seen: 2 years, 4 months
Re: Inoculate then shake?? [Re: STR80180]
    #14238507 - 04/04/11 08:49 PM (12 years, 9 months ago)

I had read that you should shake right after innoc to spread it out, but yesterday, I read that by shaking it up, you're reducing the chance of myc finding compatible myc to mate with.  By not shaking, you're keeping all the myc together, increasing the speed at which they get it on.

I'm about to noc up a bunch of jars and don't plan on shaking.


--------------------
SECURITY:  READ THIS!


Chef:  Kids, what did I tell you about drugs?
Kids:  There's a time and a place for everything, and it's called college.

How to be a good shroomie    How to grow mushrooms    A collection of good links (may be outdated)    How things should look    How to pass a drug test



Extras: Filter Print Post Top
Invisibleawakendone
Male User Gallery

Registered: 08/05/10
Posts: 824
Re: Inoculate then shake?? [Re: quebus]
    #14238528 - 04/04/11 08:55 PM (12 years, 9 months ago)

with a spore syringe you dont want to shake. with an LC it's ok to shake, but some people dont.
since 3 of your jars were with spores, it's probably better that you didnt. just leave all the jars alone, and you should be fine :thumbup:


Extras: Filter Print Post Top
Offlinezzantium2999
Shroom Groomer
Male User Gallery


Registered: 03/22/11
Posts: 177
Loc: Toronto Canada Flag
Last seen: 12 years, 2 months
Re: Inoculate then shake?? [Re: awakendone]
    #14238586 - 04/04/11 09:04 PM (12 years, 9 months ago)

whats LC?


--------------------
A good friend would bail you out of jail, but your best friend would be the one sitting next to you saying, "damn that was awesome".


Extras: Filter Print Post Top
Offlinehamloaf
Loaf of Fam.
Male User Gallery

Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma. Flag
Last seen: 1 year, 8 months
Trusted Cultivator
Re: Inoculate then shake?? [Re: Trippy_Smurf]
    #14238597 - 04/04/11 09:06 PM (12 years, 9 months ago)

Quote:

Trippy_Smurf said:
I had read that you should shake right after innoc to spread it out, but yesterday, I read that by shaking it up, you're reducing the chance of myc finding compatible myc to mate with.  By not shaking, you're keeping all the myc together, increasing the speed at which they get it on.

I'm about to noc up a bunch of jars and don't plan on shaking.



Compatible mates have already been found being that LC's a live culture so it's safe to shake upon inoculation.  If you'v already inoculated hours ago, I'd just leave it alone.  Let it do it's thing and next time I inoculated a sterile grain medium with a live culture, I'd be sure to shake upon inoculation. 


It's been forever since I'v inoculated grains with spores.  When I used to do that, the practice of a slight shake upon inoculation was employed to spread the spores around the substrate, creating more inoculation points.  More importantly IMHO, the shake upon inoculation was employed to distribute evenly, through out the grains, the extra moisture introduced by inoculating with a liquid inoculant, decreasing vectors for fermentation and mold to set in from moisture pooling at the bottom of the bag(s) or jar(s).


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


Extras: Filter Print Post Top
Invisiblehiggledy-piggledy
Registered: 08/24/10
Posts: 966
Re: Inoculate then shake?? [Re: Trippy_Smurf]
    #14238749 - 04/04/11 09:29 PM (12 years, 9 months ago)

Quote:

Trippy_Smurf said:
...I read that by shaking it up, you're reducing the chance of myc finding compatible myc to mate with.



if you inoculate with a spore suspension there will definitely land more than 1 spore on a piece of grain.

so, despite of shaking, they are close enough together to meet.  :inlove:

when inoculate with spores: shaking, no shaking - doesnt make a difference imo.


Extras: Filter Print Post Top
OfflineMycjunky
Stranger


Registered: 07/25/09
Posts: 1,837
Last seen: 3 years, 7 months
Re: Inoculate then shake?? [Re: hamloaf]
    #14238770 - 04/04/11 09:32 PM (12 years, 9 months ago)

Agree with exactly what hamloaf said. You don't want any liquid sitting at the bottom so personally I prefer to shake them after innoc.


Extras: Filter Print Post Top
Offlinequebus
Stranger

Registered: 05/01/10
Posts: 678
Last seen: 2 months, 23 days
Re: Inoculate then shake?? [Re: zzantium2999]
    #14238776 - 04/04/11 09:33 PM (12 years, 9 months ago)

Quote:

zzantium2999 said:
whats LC?




LC stands for liquid culture.  I'll post a link to a tek but basically you inject spores from a spore syringe into a water/honey mixture and the mycelium grows in the liquid.  You then take a large syringe with a large needle and transfer the mycelium to agar or grain or another liquid material.  It's a way to propagate mycelium.

Read this link.  It's a method that does not require a Pressure Cooker.  I see from your post count that you are (or appear to be) new here.  You should start with the PF TEK then move forward from there.  Good luck.


Extras: Filter Print Post Top
Offlineafrosheen
9Lives the cat
Male User Gallery


Registered: 03/06/10
Posts: 1,878
Last seen: 2 years, 4 months
Re: Inoculate then shake?? [Re: Mycjunky]
    #14238793 - 04/04/11 09:35 PM (12 years, 9 months ago)

Shake with LC, shake with spores. Spores will find mates and hook up no matter how far apart they are. Your syringe probably already has mating happening at the time of inoculation.


--------------------


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* . SouthPArk 1,376 8 05/18/05 09:52 AM
by agar
* 7 days after inoculation...nothing
( 1 2 all )
astabooty 2,727 20 09/30/05 09:19 PM
by Roadkill
* Temp increaed to HIGH!! newmodel 515 3 07/23/05 07:21 PM
by coda
* SHAKING WBS/RYE JARS -- WHEN & WHY
( 1 2 all )
agar 8,551 21 06/17/05 11:05 PM
by ezozo
* Shaking LC jars after Inoculation? jtk 2,335 3 04/22/05 11:29 AM
by scatmanrav
* SHAKING SPAWN BAGS & JARS agar 24,450 14 03/07/15 01:02 PM
by bw86
* inoculation BlueBus 1,095 7 07/18/02 05:13 AM
by BlueBus
* How often do you shake wbs jars Ascension 4,268 10 10/09/05 08:36 AM
by blackout

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
960 topic views. 20 members, 167 guests and 23 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.02 seconds spending 0.007 seconds on 14 queries.