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Karate_donkey
Pharmer


Registered: 11/18/10
Posts: 123
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Just a few ?'s about clones, prints, and degradation.
#14237561 - 04/04/11 06:10 PM (12 years, 9 months ago) |
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So I just did my second pf tek grow. It went well. I cloned one of my texas shrooms to a LC using cream corns tek. In the tek he say to use the first LC as a master and spawn it to other LC's to use so that the generation stays as the 2nd. I have read that the quality goes down the further the generation goes. My question is what qualifies as a next generation and how far can you take it? is a print another generation? can you not just keep taking prints forever? cloning forever? what about G2G?
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anonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
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Re: Just a few ?'s about clones, prints, and degradation. [Re: Karate_donkey]
#14237640 - 04/04/11 06:21 PM (12 years, 9 months ago) |
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You can keep taking prints forever.
You can't clone forever, but it goes a long ways. You don't even have to worry about it.
I'd look into doing g2g from masters tho instead of LC. It's the better route to go.
-------------------- The above post is fictional, hypothetical, or downright nonsensical.
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SomeGuy
I feel better now :)


Registered: 04/18/10
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Re: Just a few ?'s about clones, prints, and degradation. [Re: anonjon]
#14237655 - 04/04/11 06:23 PM (12 years, 9 months ago) |
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when you take a print, you go back to gen. 1
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Karate_donkey
Pharmer


Registered: 11/18/10
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Re: Just a few ?'s about clones, prints, and degradation. [Re: SomeGuy]
#14237681 - 04/04/11 06:26 PM (12 years, 9 months ago) |
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what are the advantages of a print vs cloning?
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anonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
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Re: Just a few ?'s about clones, prints, and degradation. [Re: Karate_donkey]
#14237711 - 04/04/11 06:30 PM (12 years, 9 months ago) |
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Quote:
Karate_donkey said: what are the advantages of a print vs cloning?
None, the advantage is in cloning. You get nice even flushes which gives u better yield.
When you grow from spores you r sending the organism back to sexual reproduction. It's called anastasmosis, the random shuffling of genes. It rejuvenates the dna.
It's the difference between cloning someone and mating them to make babies. Altho with fungi spores from the same fruit can mate with themselves indefinitely.
-------------------- The above post is fictional, hypothetical, or downright nonsensical.
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Karate_donkey
Pharmer


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Re: Just a few ?'s about clones, prints, and degradation. [Re: anonjon]
#14237736 - 04/04/11 06:33 PM (12 years, 9 months ago) |
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Using a print is better than using a MS syringe, right? Or is it the same thing?
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Karate_donkey
Pharmer


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Re: Just a few ?'s about clones, prints, and degradation. [Re: Karate_donkey]
#14238015 - 04/04/11 07:29 PM (12 years, 9 months ago) |
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Can you g2g forever?
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Primal Call
Earth Mage



Registered: 09/05/10
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Re: Just a few ?'s about clones, prints, and degradation. [Re: Karate_donkey]
#14238040 - 04/04/11 07:33 PM (12 years, 9 months ago) |
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Quote:
Karate_donkey said: Can you g2g forever?
hypothetically, no. Stamets recommends no more than 10 generations. do some searches on replicate fading or senescence.
Quote:
Karate_donkey said: Using a print is better than using a MS syringe, right? Or is it the same thing?
MS syringe = multispore (print) in solution.
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RogerRabbit
Bans for Pleasure



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Re: Just a few ?'s about clones, prints, and degradation. [Re: Primal Call]
#14238387 - 04/04/11 08:27 PM (12 years, 9 months ago) |
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Quote:
hypothetically, no. Stamets recommends no more than 10 generations. do some searches on replicate fading or senescence.
Hopefully, he didn't slaughter established mycology nomenclature by using the word 'generation'. A generation is when you go from spores to fruits and back to spores again. It has nothing to do with grain to grain transfers, of which 5 is plenty. After that you'll see senescence. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Primal Call
Earth Mage



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Re: Just a few ?'s about clones, prints, and degradation. [Re: RogerRabbit]
#14238464 - 04/04/11 08:43 PM (12 years, 9 months ago) |
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my mistake. I'm glad you caught that RR. I should have used the word expansion.
However, I thought that once you went back to spores you were at the beginning of a new generation, not the 2nd/3rd/etc generation of the original culture.
ie: agar to grain to bulk to fruit = generation 1. cloning from generation 1 would make for the 2nd generation and so on.
is this on?
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anonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
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Re: Just a few ?'s about clones, prints, and degradation. [Re: Primal Call]
#14238548 - 04/04/11 08:59 PM (12 years, 9 months ago) |
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No you still don't get it. But u can use the word in that sense if you specify generations of clones. We know what you mean.
In RR's world the word generation can only have one specific meaning involving sexual reproduction. If you clone a clone you're just expanding tissue culture, not creating 'generations'. Kinda rigid, but wutever.
-------------------- The above post is fictional, hypothetical, or downright nonsensical.
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afrosheen
9Lives the cat



Registered: 03/06/10
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Re: Just a few ?'s about clones, prints, and degradation. [Re: anonjon]
#14238949 - 04/04/11 10:01 PM (12 years, 9 months ago) |
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Quote:
anonjon said: No you still don't get it. But u can use the word in that sense if you specify generations of clones. We know what you mean.
In RR's world the word generation can only have one specific meaning involving sexual reproduction. If you clone a clone you're just expanding tissue culture, not creating 'generations'. Kinda rigid, but wutever.
I wouldn't call it rigid, I'd call it accurate. Generations are just like people, you're starting with a fresh new set of babies after mating occurs.
I know sometimes it seems like it's just semantics, but when you get into topics like senescence, it's important to be accurate I think.
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RogerRabbit
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Re: Just a few ?'s about clones, prints, and degradation. [Re: RogerRabbit]
#14239280 - 04/04/11 11:00 PM (12 years, 9 months ago) |
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A clone would continue the same generation.
You represent a generation. Your parents are a generation. Your grandparents are a generation, etc. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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