Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Bulk Substrate   PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Capsules   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: 1 | 2 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineOneU

Registered: 03/19/11
Posts: 763
Last seen: 12 years, 13 days
I need help with sterilization. Will this work??
    #14234447 - 04/04/11 01:10 AM (12 years, 10 months ago)

I've got everything ready and I only have one window to do this then I have a busy busy few weeks ahead of me, can't even stay near a stove for 90 mins. I read that people say use a pot. Can I use a stock pot? This is different from a crock pot which is electrical and makes veggies and chicken, a stock pot is a tall pot.



Will this work? I have another pot but it has a hole in the lid for steam escape, seems like that would defeat the purpose?

And I used search button, people keep referring to a stock pot as a crock pot which is very much different. I need a definitive answer please.

Thanks so much in advance!

Extras: Filter Print Post Top
Offlinethelivingfreekshow
Fuck You
Male User Gallery


Registered: 02/07/11
Posts: 2,043
Loc: Prifddinas, Gielinor
Last seen: 5 years, 2 months
Re: I need help with sterilization. Will this work?? [Re: OneU]
    #14234459 - 04/04/11 01:13 AM (12 years, 10 months ago)

yes that will work fine, ive never heard of a crock pot being used...:shrug:, doing pf tek i steam in a pot as well:thumbup:

Extras: Filter Print Post Top
OfflineEgoDeath0290
Chemically Dependent
Male

Registered: 04/03/11
Posts: 103
Loc: Great American Desert
Last seen: 12 years, 8 months
Re: I need help with sterilization. Will this work?? [Re: OneU]
    #14234463 - 04/04/11 01:14 AM (12 years, 10 months ago)

For PF Tek? Yes, it should work, although actual sterilization with a PC (that can reach sterile temps) is undeniably superior. You'll probably see steam escaping from the device pictured as well.


--------------------
SWIM made me say/do/post it.

Extras: Filter Print Post Top
OfflineOneU

Registered: 03/19/11
Posts: 763
Last seen: 12 years, 13 days
Re: I need help with sterilization. Will this work?? [Re: EgoDeath0290]
    #14234505 - 04/04/11 01:31 AM (12 years, 10 months ago)

it's all i got really. i have the other pot but it seems to be less air tight.

also why does rogerrabbit say don't put jars in water (even though he tapes and wraps in aluminum foil) and everyone else says put it 1/3-1/2 in the water??? i have stacked the lids and foil (2 layers) like the vid but i am so confused here and very tired i need to do this asap

Extras: Filter Print Post Top
Offlinethelivingfreekshow
Fuck You
Male User Gallery


Registered: 02/07/11
Posts: 2,043
Loc: Prifddinas, Gielinor
Last seen: 5 years, 2 months
Re: I need help with sterilization. Will this work?? [Re: OneU]
    #14234518 - 04/04/11 01:39 AM (12 years, 10 months ago)

I keep my jars OUT of the water use the STOCK pot its what i use on PF TEK, just dont boil the pot dry adding water if needed. I do the foil and stacked lids it works.:thumbup:

Extras: Filter Print Post Top
OfflineEgoDeath0290
Chemically Dependent
Male

Registered: 04/03/11
Posts: 103
Loc: Great American Desert
Last seen: 12 years, 8 months
Re: I need help with sterilization. Will this work?? [Re: OneU]
    #14234521 - 04/04/11 01:41 AM (12 years, 10 months ago)

Stack em on top of a few jar lids, and then add just enough water such that the water level is just touching the bottom of the jars. It is the steam that does the sterilizing, not the water. Just make sure steam is being produced the entire time, and go for 90 minutes after you're up to temp.

Also, if I recall correctly, RogerRabbit's tek doesn't call for any tape. That's what the dry verm layer is for. The foil is just there so that water condensation doesn't make its way into the verm layer.


--------------------
SWIM made me say/do/post it.

Edited by EgoDeath0290 (04/04/11 01:43 AM)

Extras: Filter Print Post Top
OfflineOneU

Registered: 03/19/11
Posts: 763
Last seen: 12 years, 13 days
Re: I need help with sterilization. Will this work?? [Re: EgoDeath0290]
    #14234538 - 04/04/11 01:51 AM (12 years, 10 months ago)

ok thanks so much guys i will do that. i will keep them out of the water as well and yeah it has micropore tape in the videos. watched them a few times.

thanks again!!!! <33333

:peace:

:mushroom2:

Extras: Filter Print Post Top
InvisibleLeopardMan
Constantly changing
Male


Registered: 09/21/09
Posts: 5,463
Loc: A tree house
Trusted Cultivator
Re: I need help with sterilization. Will this work?? [Re: OneU]
    #14234544 - 04/04/11 01:54 AM (12 years, 10 months ago)

It's perfect. Good luck :super:


--------------------



You have to die a few times before you can really live.

-Charles Bukowski-

Extras: Filter Print Post Top
OfflineOneU

Registered: 03/19/11
Posts: 763
Last seen: 12 years, 13 days
Re: I need help with sterilization. Will this work?? [Re: LeopardMan]
    #14234547 - 04/04/11 01:55 AM (12 years, 10 months ago)

thanks i'm off to it you guys are awesome btw.

Extras: Filter Print Post Top
OfflineEgoDeath0290
Chemically Dependent
Male

Registered: 04/03/11
Posts: 103
Loc: Great American Desert
Last seen: 12 years, 8 months
Re: I need help with sterilization. Will this work?? [Re: OneU]
    #14234574 - 04/04/11 02:08 AM (12 years, 10 months ago)

Quote:

OneU said:
ok thanks so much guys i will do that. i will keep them out of the water as well and yeah it has micropore tape in the videos. watched them a few times.

thanks again!!!! <33333

:peace:

:mushroom2:




Thought you were doing the PF Tek. Nevermind. (foot in mouth)


--------------------
SWIM made me say/do/post it.

Extras: Filter Print Post Top
InvisibleLeopardMan
Constantly changing
Male


Registered: 09/21/09
Posts: 5,463
Loc: A tree house
Trusted Cultivator
Re: I need help with sterilization. Will this work?? [Re: EgoDeath0290]
    #14234596 - 04/04/11 02:25 AM (12 years, 10 months ago)

Quote:

EgoDeath0290 said:
Also, if I recall correctly, RogerRabbit's tek doesn't call for any tape. That's what the dry verm layer is for. The foil is just there so that water condensation doesn't make its way into the verm layer.




No, you are wrong. Micropore tape is useful during the inoculation process, then you can take it off.


--------------------



You have to die a few times before you can really live.

-Charles Bukowski-

Extras: Filter Print Post Top
Offlineshroomie_glen
RedHotPussyLiquor
Male User Gallery


Registered: 03/01/06 Happy 18th Shroomiversary!
Posts: 4,296
Loc: Narf Carolina Flag
Last seen: 11 years, 4 months
Re: I need help with sterilization. Will this work?? [Re: LeopardMan]
    #14234645 - 04/04/11 02:51 AM (12 years, 10 months ago)

Don't use this steam method for anything BUT pf tek.. It will not be relatively sucessful for grains and other spawn substrates.


--------------------

No. No, man. Shit, no man.  I believe you'd get your ass kicked sayin' somethin' like that man.

Extras: Filter Print Post Top
Invisiblehamloaf
Pork Block
Male User Gallery


Registered: 12/23/09
Posts: 20,575
Loc: Oklahoma. Flag
Trusted Cultivator
Re: I need help with sterilization. Will this work?? [Re: LeopardMan]
    #14234683 - 04/04/11 03:16 AM (12 years, 10 months ago)

Quote:

LeopardMan said:
Quote:

EgoDeath0290 said:
Also, if I recall correctly, RogerRabbit's tek doesn't call for any tape. That's what the dry verm layer is for.




No, you are wrong. Micropore tape is useful during the inoculation process, then you can take it off.





Actually I think you both are incorrect.  In RR's videos, micropore tape is clearly called for as Jim puts it on pre sterilization.  The micropore tape's purpose for use with BRF cakes constructed per the tek are not an aid in the inoculation process.  Moreover, micropore tape's purpose for properly constructed BRF cakes are a means of filtered ventilation for whenever you steam sterilize or PC your cakes and they are cooling down, de-pressurizing because BRF cakes designed per the tek's lids are rubber side down. 


Quote:

The foil is just there so that water condensation doesn't make its way into the verm layer.





This is partially correct.  The foil is used to protect the dripping condensation that forms on the bottom of the sterilizer's lid during the sterilization cycle that rains down onto the jars from moistening the micropore tape and possible moisture introduction to the dry vermiculite barrier from that, yes.  The foil is also used to keep the boiling water from the bottom of the sterilizer from being thrusted between the lids and threads of jar during sterilization, also causing your dry vermiculite barrier to become moistened.  I'm always sure to keep the foil removed during colonization nor do I replace the micropore tape for colonization.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.

Extras: Filter Print Post Top
OfflineEgoDeath0290
Chemically Dependent
Male

Registered: 04/03/11
Posts: 103
Loc: Great American Desert
Last seen: 12 years, 8 months
Re: I need help with sterilization. Will this work?? [Re: hamloaf]
    #14234697 - 04/04/11 03:28 AM (12 years, 10 months ago)

Quote:

hamloaf said:
Quote:

LeopardMan said:
Quote:

EgoDeath0290 said:
Also, if I recall correctly, RogerRabbit's tek doesn't call for any tape. That's what the dry verm layer is for.




No, you are wrong. Micropore tape is useful during the inoculation process, then you can take it off.





Actually I think you both are incorrect.  In RR's videos, micropore tape is clearly called for as Jim puts it on pre sterilization.  The micropore tape's purpose for use with BRF cakes constructed per the tek are not an aid in the inoculation process.  Moreover, micropore tape's purpose for properly constructed BRF cakes are a means of filtered ventilation for whenever you steam sterilize or PC your cakes and they are cooling down, de-pressurizing because BRF cakes designed per the tek's lids are rubber side down. 


Quote:

The foil is just there so that water condensation doesn't make its way into the verm layer.





This is partially correct.  The foil is used to protect the dripping condensation that forms on the bottom of the sterilizer's lid during the sterilization cycle that rains down onto the jars from moistening the micropore tape and possible moisture introduction to the dry vermiculite barrier from that, yes.  The foil is also used to keep the boiling water from the bottom of the sterilizer from being thrusted between the lids and threads of jar during sterilization, also causing your dry vermiculite barrier to become moistened.  I'm always sure to keep the foil removed during colonization nor do I replace the micropore tape for colonization.




Is the tape considered optional, or mandatory?

That's what I get for watching abbreviated material on youtube rather than forking out the money for the full DVD. totally missed that part, but now I can see clearly that there is tape over the lid. Thanks for clarifying.


--------------------
SWIM made me say/do/post it.

Edited by EgoDeath0290 (04/04/11 03:35 AM)

Extras: Filter Print Post Top
InvisibleLeopardMan
Constantly changing
Male


Registered: 09/21/09
Posts: 5,463
Loc: A tree house
Trusted Cultivator
Re: I need help with sterilization. Will this work?? [Re: hamloaf]
    #14234710 - 04/04/11 03:35 AM (12 years, 10 months ago)

Quote:

hamloaf said:
Actually I think you both are incorrect.  In RR's videos, micropore tape is clearly called for as Jim puts it on pre sterilization.  The micropore tape's purpose for use with BRF cakes constructed per the tek are not an aid in the inoculation process.





:nono: "The purpose of the micropore tape is to protect the top of the verm layer until you inoculate.  If you don't use it, there's the possibility that mold spores will slip through the holes in the lid and land on the verm, and then your syringe will push it through to the rice flour below."(RR).

That's why micropore tape is useful for the inoculation process.


--------------------



You have to die a few times before you can really live.

-Charles Bukowski-

Extras: Filter Print Post Top
Invisiblehamloaf
Pork Block
Male User Gallery


Registered: 12/23/09
Posts: 20,575
Loc: Oklahoma. Flag
Trusted Cultivator
Re: I need help with sterilization. Will this work?? [Re: EgoDeath0290]
    #14234711 - 04/04/11 03:35 AM (12 years, 10 months ago)

Quote:

Is the tape considered optional, or mandatory?





Depends on whether or not you design the cakes per the tek or not.  If you do then yes, the tape's mandatory.  If you fit BRF cakes with properly constructed grain jar lids for colonization then no, the tape has no place.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.

Extras: Filter Print Post Top
OfflineEgoDeath0290
Chemically Dependent
Male

Registered: 04/03/11
Posts: 103
Loc: Great American Desert
Last seen: 12 years, 8 months
Re: I need help with sterilization. Will this work?? [Re: hamloaf]
    #14234721 - 04/04/11 03:38 AM (12 years, 10 months ago)

Gotcha. Its funny how subtle that detail is in the quickie youtube vids. Now I know I need the DVD.

Quote:

The purpose of the micropore tape is to protect the top of the verm layer until you inoculate.  If you don't use it, there's the possibility that mold spores will slip through the holes in the lid and land on the verm, and then your syringe will push it through to the rice flour below."(RR).




At which point during the process is this most likely to happen? When removing the foil inside the still-air box?

Any decent MP tape alternatives you'd suggest? Like the tape I use to wrap the balls of my feet for cross country running?


--------------------
SWIM made me say/do/post it.

Edited by EgoDeath0290 (04/04/11 03:47 AM)

Extras: Filter Print Post Top
Invisiblehamloaf
Pork Block
Male User Gallery


Registered: 12/23/09
Posts: 20,575
Loc: Oklahoma. Flag
Trusted Cultivator
Re: I need help with sterilization. Will this work?? [Re: LeopardMan]
    #14234747 - 04/04/11 03:55 AM (12 years, 10 months ago)

Quote:

LeopardMan said:

:nono: "The purpose of the micropore tape is to protect the top of the verm layer until you inoculate.  If you don't use it, there's the possibility that mold spores will slip through the holes in the lid and land on the verm, and then your syringe will push it through to the rice flour below."(RR).

That's why micropore tape is useful for the inoculation process.



This makes sense to me.  You swear you'r RR when you use the :nono: though.  However, I stand by my position that another purpose for the micropore tape in BRF cakes constructed per the tek is to keep the air being sucked into the jars clean while they are cooling due to the lids being rubber side down.  Good day to you and Happy Shrooming.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.

Extras: Filter Print Post Top
OfflineOneU

Registered: 03/19/11
Posts: 763
Last seen: 12 years, 13 days
Re: I need help with sterilization. Will this work?? [Re: hamloaf]
    #14235941 - 04/04/11 12:29 PM (12 years, 10 months ago)

so they've been cooling down. is it ok to leave them in the water in the pot?? also it's not an air lock lid so is it ok to be stored on a shelf that has been wiped down with iso + lysol wipes? 

and can i move them into the GB with the foil on, remove it in there and noc them up? would i need to spray the room with disinfectant? (i have lysol spray idk why oust is more recommended)

my syringe is already 2 days late from VERY RELIABLE vendor from here :sad:

Edited by OneU (04/04/11 02:00 PM)

Extras: Filter Print Post Top
Invisiblecyantific
Trusted Masturbator


Registered: 10/11/09
Posts: 1,323
Loc: Shakashuri Island
Re: I need help with sterilization. Will this work?? [Re: OneU]
    #14237977 - 04/04/11 07:18 PM (12 years, 10 months ago)

what do you consider late ? was there a tracking number or are you guesstimating ?

you can leave them for a pretty good amount of time with the foil on ... and yes i always leave them on till already in the GB ... just makes sense to keep the lid contaminate free ...

as for sterilizing work areas and storage ... knock yourself out ... what you plan is more than enough safety measure ...

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | Next >  [ show all ]

Shop: North Spore Bulk Substrate   PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Capsules   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* Need advice on sterility & trading spores. The_Gnome_King 4,371 16 06/25/20 05:22 AM
by Zakkery
* sterilizing straw in oven bags Sterile 2,058 12 03/15/05 08:04 AM
by Sterile
* Every how often do i have to sterilize the misting water? Sterile 2,792 8 10/24/23 12:30 PM
by 10ftTall
* Micropore Tape distortion 4,040 13 04/21/03 01:09 AM
by CultyVader
* Dishwasher Sterilization Technique djcontra 4,566 5 01/19/03 04:19 AM
by Krayzie
* How to sterilize gloves DNA 9,248 7 05/06/04 03:10 AM
by Magash
* Sterile water... LouiseLouise 3,532 15 03/16/21 12:21 AM
by Benson
* Jars sterile, but what about terrarium? Stevo21 1,067 4 04/07/04 05:36 PM
by OJK

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,327 topic views. 36 members, 187 guests and 45 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.033 seconds spending 0.008 seconds on 13 queries.