Home | Community | Message Board

Sporeworks
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Substrate Bags

Jump to first unread post Pages: 1 | 2 | 3 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineMike1234
Trusted Agent

Registered: 02/25/11
Posts: 14
Last seen: 8 years, 8 months
PF TEK WITHOUT Sterilization of Substrate
    #14232557 - 04/03/11 06:35 PM (12 years, 9 months ago)

I created 8 jars using PF TEK procedure exactly except I did not sterilize my substrate - i.e., I did not put the filled jars into a pressure cooker or anything before innoculating them.

Result - 100% Contamination with greens, reds, blacks, yellows - a virtual rainbow of contamination.

I then burried the contaminated cakes - I will let you know if any thing grows or I die.

Next, I am going to do the same thing, with the same jars, but this time I'am going to use Hydrogen Peroxide.


Extras: Filter Print Post Top
Offlinedeadmandave
Slime
Male User Gallery

Registered: 02/16/10
Posts: 3,355
Loc: Flag
Last seen: 7 hours, 34 minutes
Trusted Cultivator
Re: PF TEK WITHOUT Sterilization of Substrate [Re: Mike1234]
    #14232567 - 04/03/11 06:37 PM (12 years, 9 months ago)

What is the purpose of this experiment?


Extras: Filter Print Post Top
Offlinesickofit
*statement*
Male User Gallery

Registered: 12/01/10
Posts: 348
Loc: Local Park Flag
Last seen: 1 year, 4 months
Re: PF TEK WITHOUT Sterilization of Substrate [Re: Mike1234]
    #14232571 - 04/03/11 06:38 PM (12 years, 9 months ago)



--------------------
A Resource on Psi, Science, and Philosophy
The Taboo of Psi
Trade/Want List
Mechanisms and reactions
Is ____ down, or is it just me?
You know, its against the rules to discuss/trade research chemical sources on the board, oh hey look,a "contact user" button, isn't this site the greatest!
Done(In order):
Nicotine, THC, Psilocybin (Cubes,P. Cyans,Pan. Cinctulus), MDMA, LSD, 2C-E, Methampetamine, Lost track of how many JWH's, MDA, Nitrous Oxide, 2C-I, Cocaine, Ketamine, TMA-6, 2C-B, DOC, DXM, 4-MMC, MDAI, MDAT, MDPV, butylone, pentedrone, MXE, 25i, 25c, 25b, hydromorphone, 2 and 4-FA, 2 and 4 FMA, 6-apb, DMT


Extras: Filter Print Post Top
Offlineesquaredx
 User Gallery


Registered: 10/24/09
Posts: 1,497
Loc: Dutch Country
Last seen: 2 years, 4 months
Re: PF TEK WITHOUT Sterilization of Substrate [Re: sickofit]
    #14232611 - 04/03/11 06:45 PM (12 years, 9 months ago)

:facepalm:



Just do this right if your looking for some fruits.


--------------------


Extras: Filter Print Post Top
OfflineMike1234
Trusted Agent

Registered: 02/25/11
Posts: 14
Last seen: 8 years, 8 months
Re: PF TEK WITHOUT Sterilization of Substrate [Re: sickofit]
    #14232624 - 04/03/11 06:47 PM (12 years, 9 months ago)

2 Fold;

1)  What reduction in contamination is seen from Hydrogen Peroxide

2)  What is the success rate of moving contaminated cakes to an outdoor environment (Pasturized Cow Dung and Straw)


Extras: Filter Print Post Top
Invisiblebiologys
Mycologist in Trainning
Male


Folding@home Statistics
Registered: 12/21/09
Posts: 4,622
Re: PF TEK WITHOUT Sterilization of Substrate [Re: Mike1234]
    #14232630 - 04/03/11 06:48 PM (12 years, 9 months ago)

you're going to have a zero% success rate...


mold is eating your substrate before the spores of mushrooms even has a chance to think about mating and colonizing..


Extras: Filter Print Post Top
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: PF TEK WITHOUT Sterilization of Substrate [Re: Mike1234]
    #14232633 - 04/03/11 06:48 PM (12 years, 9 months ago)

Established procedures became standard practice because they work.
It's great to reinvent the wheel from time to time, but be aware there are great teks to be followed.


--------------------
You make it all possible. Doesn't it feel good?


Extras: Filter Print Post Top
OfflineMike1234
Trusted Agent

Registered: 02/25/11
Posts: 14
Last seen: 8 years, 8 months
Re: PF TEK WITHOUT Sterilization of Substrate [Re: Doc_T]
    #14232665 - 04/03/11 06:53 PM (12 years, 9 months ago)

Added piece of info - all 8 cakes were heavily colonized with my mycelium


Extras: Filter Print Post Top
Invisiblebiologys
Mycologist in Trainning
Male


Folding@home Statistics
Registered: 12/21/09
Posts: 4,622
Re: PF TEK WITHOUT Sterilization of Substrate [Re: Mike1234]
    #14232671 - 04/03/11 06:54 PM (12 years, 9 months ago)

if you didn't sterilize, i'm willing to bet evrything i have that what you saw was trich mycelium, not mushroom..


Extras: Filter Print Post Top
Offlineesquaredx
 User Gallery


Registered: 10/24/09
Posts: 1,497
Loc: Dutch Country
Last seen: 2 years, 4 months
Re: PF TEK WITHOUT Sterilization of Substrate [Re: biologys]
    #14232689 - 04/03/11 06:57 PM (12 years, 9 months ago)

Sounds like someone is..
:awehigh:



This is a waste of time.


--------------------


Extras: Filter Print Post Top
OfflineMike1234
Trusted Agent

Registered: 02/25/11
Posts: 14
Last seen: 8 years, 8 months
Re: PF TEK WITHOUT Sterilization of Substrate [Re: biologys]
    #14232882 - 04/03/11 07:36 PM (12 years, 9 months ago)

Ok, I admit to being completely new to this; I was not aware that Trich produced mycelium or that Trich would appear as similar to mushroom mycelium?


Extras: Filter Print Post Top
Invisiblebiologys
Mycologist in Trainning
Male


Folding@home Statistics
Registered: 12/21/09
Posts: 4,622
Re: PF TEK WITHOUT Sterilization of Substrate [Re: Mike1234]
    #14232898 - 04/03/11 07:39 PM (12 years, 9 months ago)

dude, goto www.mushroomvideos.com and watch the video's it'll teach you how to do the proper way..


Extras: Filter Print Post Top
OfflineMike1234
Trusted Agent

Registered: 02/25/11
Posts: 14
Last seen: 8 years, 8 months
Re: PF TEK WITHOUT Sterilization of Substrate [Re: biologys]
    #14232974 - 04/03/11 07:53 PM (12 years, 9 months ago)

The PF TEK is not a mystery - it is explained clearly on this site.

It is not that I don't understand the technique, it is that I choose to make one change and test the results.

It may be a waste of time and resources - I am ok with that; that is part of the cost of experimenting. 

The cakes were all solid white and very well held together by what ever what strand like mycelium had taken them over.  Each instance of mold was isolated to an area no bigger than a dime in most cases or much smaller.

I will let you know how it all comes out.


Extras: Filter Print Post Top
OfflineMad River
Reverend, Churchof Todd
Male User Gallery

Registered: 04/29/08
Posts: 1,114
Loc: The Great Lakes Region, U...
Last seen: 2 years, 6 months
Re: PF TEK WITHOUT Sterilization of Substrate [Re: Mike1234]
    #14233014 - 04/03/11 08:02 PM (12 years, 9 months ago)

Experiment 2: I severed my middle finger using a rusty, dull razorblade.

Results: 100% missing middle finger. My hand is bleeding, I have tetanus, and a nasty infection.

Thank you for this important work.


Extras: Filter Print Post Top
Offlinehealing
Strangest
Female

Registered: 02/22/11
Posts: 6,565
Loc: the universe, the milky w...
Last seen: 6 years, 6 months
Re: PF TEK WITHOUT Sterilization of Substrate [Re: Mike1234]
    #14233018 - 04/03/11 08:02 PM (12 years, 9 months ago)

The mushroom videos are a much better way to learn to produce mushrooms.


--------------------
Open mind, open heart, open book.



Extras: Filter Print Post Top
OfflineCyanescensRule
DayTripper


Registered: 12/15/10
Posts: 44
Last seen: 11 years, 7 months
Re: PF TEK WITHOUT Sterilization of Substrate [Re: Mad River]
    #14233181 - 04/03/11 08:36 PM (12 years, 9 months ago)

Quote:

Mad River said:
Experiment 2: I severed my middle finger using a rusty, dull razorblade.

Results: 100% missing middle finger. My hand is bleeding, I have tetanus, and a nasty infection.

Thank you for this important work.




:laugh2:


--------------------
:bow2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::lsdabc::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::bow2:


Extras: Filter Print Post Top
Offlinebakenast
Muffen Stuffer
 User Gallery

Registered: 03/14/11
Posts: 920
Last seen: 1 year, 26 days
Re: PF TEK WITHOUT Sterilization of Substrate [Re: Mike1234]
    #14233398 - 04/03/11 09:12 PM (12 years, 9 months ago)

Quote:

Mike1234 said:
The PF TEK is not a mystery - it is explained clearly on this site.

It is not that I don't understand the technique, it is that I choose to make one change and test the results.

It may be a waste of time and resources - I am ok with that; that is part of the cost of experimenting. 

The cakes were all solid white and very well held together by what ever what strand like mycelium had taken them over.  Each instance of mold was isolated to an area no bigger than a dime in most cases or much smaller.

I will let you know how it all comes out.



I like ur ideas tyring something new yep prolly wont work, but cool too try new things U never know mushroom can grow in poor conditions, I see no reason y It wouldnt be posible to get lucky on a jar :smile:


Extras: Filter Print Post Top
OfflineTrippy_Smurf
Sketchy Mother Fucker
Male User Gallery


Folding@home Statistics
Registered: 02/14/11
Posts: 2,349
Loc: Smurf Villiage Flag
Last seen: 2 years, 4 months
Re: PF TEK WITHOUT Sterilization of Substrate [Re: Mad River]
    #14233405 - 04/03/11 09:13 PM (12 years, 9 months ago)

Mad River
Quote:

Mad River said:
Experiment 2: I severed my middle finger using a rusty, dull razorblade.

Results: 100% missing middle finger. My hand is bleeding, I have tetanus, and a nasty infection.

Thank you for this important work.




Could you repeat the experiment, but this time, immediately after severing, pour h2o2 on it and report back whether or not it's still missing?


--------------------
SECURITY:  READ THIS!


Chef:  Kids, what did I tell you about drugs?
Kids:  There's a time and a place for everything, and it's called college.

How to be a good shroomie    How to grow mushrooms    A collection of good links (may be outdated)    How things should look    How to pass a drug test



Extras: Filter Print Post Top
Invisiblelemonfuzz
Stranger Danger
 User Gallery


Registered: 03/17/11
Posts: 90
Loc: US
Re: PF TEK WITHOUT Sterilization of Substrate *DELETED* [Re: Trippy_Smurf]
    #14233598 - 04/03/11 09:44 PM (12 years, 9 months ago)

Post deleted by lemonfuzz

Reason for deletion: .



Extras: Filter Print Post Top
OfflineOneU

Registered: 03/19/11
Posts: 763
Last seen: 11 years, 11 months
Re: PF TEK WITHOUT Sterilization of Substrate [Re: Mike1234]
    #14233627 - 04/03/11 09:49 PM (12 years, 9 months ago)

Quote:

Mike1234 said:
Added piece of info - all 8 cakes were heavily colonized with my mycelium



just an FYI, it doesn't matter if you put 10cc's of the spores in or 1-2cc's in the jar. you probably reduce the probability of a grow with more than 2. also, :lol:


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3 | Next >  [ show all ]

Shop: Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Substrate Bags


Similar ThreadsPosterViewsRepliesLast post
* PF Tek newbie substrate ? socratesmind 1,438 3 09/06/02 08:31 AM
by Anno
* Is this healthy? Current concerns. PF Tek Jars frenzylock 3,980 17 05/06/21 05:44 PM
by Ashtray161
* PF Tek question forevadazin 3,145 11 11/04/02 03:06 PM
by Knives_Out
* Can Contam Spread From Jar To Jar?? (pf tek brf) PooGrower 4,782 7 12/04/02 02:18 AM
by Bi0TeK
* casing with pre used pf tek substrate superpsilyguy 1,450 3 12/16/02 06:16 PM
by Coma of Souls
* PF-Tek or Rye grain DrD525 10,999 17 06/03/16 01:57 PM
by openmindedcanvas
* Ambiguous point in the PF tek Chibboleth 914 5 01/08/03 04:24 AM
by dog
* first timer, myco bags or reg PF Tek method?? gonzosthompson 3,055 4 10/08/02 06:09 PM
by Audi0

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
5,519 topic views. 28 members, 172 guests and 29 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.031 seconds spending 0.011 seconds on 16 queries.