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OfflineWizzy
In Tha Hizzy


Registered: 11/09/10
Posts: 852
Loc: Void Center
Last seen: 11 years, 2 months
Fuck Shaking
    #14231670 - 04/03/11 03:40 PM (12 years, 9 months ago)

Well, some dude that I met at a rock concert has this friend who's sister is really hot, and she's done shaking jars. Why? Because although they worked the FIRST time she tried it and the jars colonized faster, THIS time (and with a different strain), the jars have not been colonizing nearly as fast. It's healthy forsure, but another thing she noticed was that when innoculating via MS syringe, the growth always starts off supder duper rhizomorphic and after being shaken it's tomentose as a ma'fucka. So yeah screw shaking jars just wait and let them do their thing because in the end they usually finish fast anyways and I think shaking is really just for impatient people.
Do you shake your jars?
You may choose only one


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OfflineGrungeman17
Male


Registered: 05/06/09
Posts: 1,436
Loc: usa Flag
Last seen: 2 days, 10 hours
Re: Fuck Shaking [Re: Wizzy]
    #14231748 - 04/03/11 03:57 PM (12 years, 9 months ago)

with spawn bag the process your refering to is called "kneeding" and is a much more benificial tek doing it in bags, it alows you to be soft and gentle while redistributing myceilium thoughout your spawn material. bags are so much better than jars imo, shaking the jars is a little more rough on our white buddy..


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Invisiblebiologys
Mycologist in Trainning
Male


Folding@home Statistics
Registered: 12/21/09
Posts: 4,622
Re: Fuck Shaking [Re: Grungeman17] * 1
    #14231761 - 04/03/11 03:59 PM (12 years, 9 months ago)

as i've repeated over an over again, another good thing about shaking your jars, if you have any trich present, when you shake its going to show itself with in a couple of days...

if you do not do this there's a chance you're spawning contaminated grains to a monotub, setting yourself up for failure before you even start..


I myself shake every single jar at 20-30% and then again at full colonization


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Offlinedeadmandave
Slime
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Registered: 02/16/10
Posts: 3,355
Loc: Flag
Last seen: 7 hours, 6 minutes
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Re: Fuck Shaking [Re: biologys]
    #14231835 - 04/03/11 04:14 PM (12 years, 9 months ago)

I shake because it has always sped up colonization. I think shaking is crucial especially if inoculating with an agar wedge, spore solution or g2g.

IMHO if the jars arent colonizing as quickly something has gone wrong BESIDES the shake :biggrin:


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OfflineMicroppose
Things Maker
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Registered: 11/30/10
Posts: 849
Loc: Amongst you...
Last seen: 21 hours, 27 minutes
Re: Fuck Shaking [Re: deadmandave]
    #14231887 - 04/03/11 04:22 PM (12 years, 9 months ago)

Your friend obviously did something wrong, and fabricated an explanation. There are several good reasons why people shake their jars.


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Offlineskiddy
RockStar
Male

Registered: 03/25/11
Posts: 366
Last seen: 12 years, 6 months
Re: Fuck Shaking [Re: deadmandave]
    #14231916 - 04/03/11 04:27 PM (12 years, 9 months ago)

I 100% agree with kneeding the bags but i do not shake my jars i don't really have a good reason for this other than id rather just lets it colonize on its own and theoretical if you shake a jar it could cause a longer colonization time but not likley.

IMO shaking probably can't hurt :smile:


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PESH : Pinning
Transeski : colonizing
Orrisa : colonizing
Mex a : colonizing

You're not a mycologist just because you grow mushrooms.


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Offlineshroomtastic89
Dolla Dolla Bills Y'all
Male


Registered: 11/28/10
Posts: 573
Loc: USA
Last seen: 6 years, 10 months
Re: Fuck Shaking [Re: skiddy]
    #14232127 - 04/03/11 05:07 PM (12 years, 9 months ago)

Shake for sure... After a shake it should be 100% in a week or so... Shaking helps spread the colonized grain with the uncolonized creating more surface area of colonized grain. Thus increasing the colonization speed.


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Offlinedeadmandave
Slime
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Registered: 02/16/10
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Re: Fuck Shaking [Re: shroomtastic89]
    #14232252 - 04/03/11 05:31 PM (12 years, 9 months ago)

The only way I can think shaking would be detrimental would be ifyou shook extremely hard for a prolonged period or by shaking too many times weakening the mycelium. But otherwise one even two shakes don't do enough damage to the mycelium to measure or notice.


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OfflineAnarchyfest
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Registered: 04/18/10
Posts: 212
Loc: South of Heaven, usa Flag
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Re: Fuck Shaking [Re: deadmandave]
    #14234173 - 04/03/11 11:44 PM (12 years, 9 months ago)

This girl explained all this to? Probly unlikely but shaking jars only helps . Don't be a pie an ask her out weenie


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If you must live by the sword, then you will die by the sword.


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Invisibleslapphappypill
Enthusiast!
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Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
Re: Fuck Shaking [Re: Anarchyfest]
    #14234435 - 04/04/11 01:07 AM (12 years, 9 months ago)

I shake right after inoculation from LC, then I leave it. I've never had a problem doing it this way. If I shake at 30%, it never finishes colonizing for me...


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We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~


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Invisibleanonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
Re: Fuck Shaking [Re: slapphappypill]
    #14234859 - 04/04/11 05:33 AM (12 years, 9 months ago)

Maybe the title should be fuck liquid culture.

G2g is faster and more reliable. I used to think LC was the shit before I started making grain masters from rgs.

If your jars don't recover well after a shake, you might be making your wbs too dry. Once those millet grains dry out or get overcooked, they don't like to colonize very well.


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The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:


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Invisibleslapphappypill
Enthusiast!
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Folding@home Statistics
Registered: 11/07/10
Posts: 5,570
Loc: In bed with your mom
Re: Fuck Shaking [Re: anonjon]
    #14234884 - 04/04/11 05:52 AM (12 years, 9 months ago)

thats the thing though, my rye didn't recover 90% of the time, and my WBS recovers 95% of the time. the WBS for sure isn't too dry, and the mullet doesn't bust a whole lot.

LC has worked the best for me, so I'm not sure why you're knocking LC... I did agar to LC in open air, nocked up 20 jars, and they fruited perfectly!

Everything works differently for everyone. Thats why I've falling in love with Mycology! What works perfect for me, might not work worth shit for anyone else. As long as my babies are happy thats all I'm worried about :datass:


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~


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Invisibleanonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
Re: Fuck Shaking [Re: slapphappypill]
    #14234897 - 04/04/11 06:03 AM (12 years, 9 months ago)

True. It's a mystery tho why your jars wouldn't recover. You're doing something different.

I knock LC cuz you're the exception, not the rule. Doing LC work in the open is full on crazy imo, but if it works :shrug2:


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The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:


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Invisiblemaiko maiko
I can eat 50 eggs

Registered: 02/26/10
Posts: 332
Re: Fuck Shaking [Re: anonjon]
    #14234942 - 04/04/11 06:32 AM (12 years, 9 months ago)

i've done LC and g2g's side by side and the LC was way faster. i had 1 jar i shot up with extra LC colonize 100% in 5 days without a shake aside from immediately after inoculation. agar>LC in a still air box seems to work pretty well.

not knocking g2g. i make the transfer and roll the jars to redistribute and usually have full colonization after 10 days and 1 shake around 30%.


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Invisibleanonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
Re: Fuck Shaking [Re: maiko maiko]
    #14234950 - 04/04/11 06:40 AM (12 years, 9 months ago)

Using that much LC in each jar on a regular basis, I think you'd find the contamn rates unacceptable, especially in large spawn bags.

That was my experience. RR has said the same thing a million times.


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The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:


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