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Offlinejenia1
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Registered: 11/21/10
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Few Questions be4 im getting it Done.
    #14230813 - 04/03/11 11:42 AM (12 years, 9 months ago)

hey there guys! im sitting here writing this post and when im finish i gonna start preapering my sterile room for the job to be done
meanwhile i got a few questions  which are very crucial, atleast so i assume.

1. i have about 9 petri dishes with argentina mycillium on it some are contams some r not unfortunatlly i cant bring the pics now, and the rye jars are sterile and at room temps. what i wanna know is how many petri dish cuts i shud insert into 1 rye jar of about 500ml  because i dont know if its the same genom type or not.

2. i made afew LC jars around 250ml each and i used 2ml of liquidspore from spore syringe i made myself from a print- same question here. if i take it later to petri dish and it looks good than i can suck the LC with  a syringe and inoculate jars with it?( meanning is the LC jar have the same genom type? or many genoms inside?)

time is of an essence. thanks in advance!


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Offlinebbt0009
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Registered: 11/11/08
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Re: Few Questions be4 im getting it Done. [Re: jenia1]
    #14230921 - 04/03/11 12:08 PM (12 years, 9 months ago)

Here's what I would do. I would take your best looking growth and cut a nice little pie slice and drop that into an LC. With the myc. already growing it'll spread in that LC extremely fast. There's too many possible contam factors in all the steps you mentioned about making your syringe. Always assume they're no good til you test them.

And if you have 9 different isolates then one chunk per jar would do. However I would try what I mentioned above.


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