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OregonChronic
Iron, Lion, Cyans



Registered: 09/12/10
Posts: 1,970
Loc: Scoping Woodchips
Last seen: 11 years, 7 days
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Spore Printing
#14230672 - 04/03/11 11:02 AM (12 years, 9 months ago) |
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I always wanted to make my own spore syringes from my own spore prints, but i think it would be hard for me to suck the spores up in the syringe while being perfectly sterile.
Correct me if im wrong but aren't you supposed to pressure cook some water in a jar and suck up the sterilized water into a syringe in a sterile environment then still in a sterile environment just squirt the sterilized water on the spores and suck back into the syringe and cap.
Is that a correct procedure to prepare a spore syringe? And can the water for the syringe be just boiled and sucked into it or does it have to be pressure cooked in a jar and sucked into the syringe in a glovebox?
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Wavy Cap Cyan Spore Prints for Trade/Sale
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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what I do, is get some water boiling hot on the stove.. suck it up into the syringe, and let sit for 3 minutes, and repeat this 3 more times.. on the 4th time, leave the water in until cool, while capping the needle..
now you have sterile water in the syringe..
then, i clean out a shot glass with alcohol, let sit for a minute, scrape some spores in the shot glass, shoot some water in, suck up, and repeat 3 times.. on the last time, you should see plenty of spores floating around in your syringe.. cap, and store/use.. lol
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OregonChronic
Iron, Lion, Cyans



Registered: 09/12/10
Posts: 1,970
Loc: Scoping Woodchips
Last seen: 11 years, 7 days
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Should i just do the squirting/sucking on the normal spore print on the foil without transferring to a shot glass or should i scrape the spores ino a glass like you do? It seems that with the scraping and putting in a shot glass contam risk is a bit higher. Ima build a glovebox to do it in. duh
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Wavy Cap Cyan Spore Prints for Trade/Sale
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Cynosure
allow me to be your guide.


Registered: 10/06/09
Posts: 4,228
Last seen: 11 months, 12 days
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I went ahead and used the shot glass method.
Shooting water onto the foil seems like it would make a pretty big mess and you don't necessarily want to use all of your spores in one syringe, so scraping them off into a shot glass helps you extend your print! =)
-------------------- "You can peel it [language] off the ceiling and make it dance in front of you" - McKenna <3 .
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SomeGuy
I feel better now :)


Registered: 04/18/10
Posts: 7,496
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Last seen: 9 years, 8 months
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Quote:
DJYoshaBYD said: what I do, is get some water boiling hot on the stove.. suck it up into the syringe, and let sit for 3 minutes, and repeat this 3 more times.. on the 4th time, leave the water in until cool, while capping the needle..
now you have sterile water in the syringe..
then, i clean out a shot glass with alcohol, let sit for a minute, scrape some spores in the shot glass, shoot some water in, suck up, and repeat 3 times.. on the last time, you should see plenty of spores floating around in your syringe.. cap, and store/use.. lol
I'm glad thi subject came up, I'm interested in other peaples methods. What I do is just what this guy does. After the veil breaks, and I see some spores on it, I mist and fan on more time. Then I wait an hour. The I take a piece of tyvek, and cut it from the middle of one side, about 75% of the way across. Then, I fold it on the cut and in the middle, make another cut, so it forms a t. Then I spray it with dissinfectant. Then I put it in a couple of pieces of toilet paper. Then, aftyer a moment, I hold my breath, and affix it around the mush to be printed. I don't spray. I don't mist. Hours later, when I can see plenty of spores have dropped, I spray my hands with lysol. I get out a new ziplock bag. I hold my breath, open the terrarium, put the bag in and open it. I put the tyvek straight in the bag and seal it. The rest I do in a glove gbox
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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Quote:
OregonChronic said: Should i just do the squirting/sucking on the normal spore print on the foil without transferring to a shot glass or should i scrape the spores ino a glass like you do? It seems that with the scraping and putting in a shot glass contam risk is a bit higher. Ima build a glovebox to do it in. duh
for sure do it in a still-air environment, like a glovebox..
as for the scraping, you should be scraping with a sterile instrument, like a sterilized knife or inoculation loop..
as long as your tools are clean, and the person that made the print knew what they were doing, your syringe will be just fine.. this is the only method I have ever used..
i actually just made 2 z-strain syringes earlier this month just like that, and they are all doing fine, as im just about to pull a flush from it.. giggity goooooo
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OregonChronic
Iron, Lion, Cyans



Registered: 09/12/10
Posts: 1,970
Loc: Scoping Woodchips
Last seen: 11 years, 7 days
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Alright nice to hear that...i will be doing this on my next grows.
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Wavy Cap Cyan Spore Prints for Trade/Sale
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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you dont need much from the syringe.. i like to use at least 1/5 of the print, though.. but i also make lots of prints when I can..
another cool way, is if you really need a syringe right off the batt, you can take a small cap, put it in a shot glass, gills down, and let it sit, covered, over night.. pull it out, and suck up the spores with sterile water..
very nice way to get a full syringe in a pinch
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Cynosure
allow me to be your guide.


Registered: 10/06/09
Posts: 4,228
Last seen: 11 months, 12 days
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Quote:
DJYoshaBYD said: another cool way, is if you really need a syringe right off the batt, you can take a small cap, put it in a shot glass, gills down, and let it sit, covered, over night.. pull it out, and suck up the spores with sterile water..

That's a very helpful technique you have there! It seems like it would definitely cut down on the chance of contamination, I'll have to give it a shot.
-------------------- "You can peel it [language] off the ceiling and make it dance in front of you" - McKenna <3 .
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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Re: Spore Printing [Re: Cynosure]
#14230779 - 04/03/11 11:34 AM (12 years, 9 months ago) |
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yeah.. i dont use it too often, but like I said, it works well when you need it.. I usually just make a shitload of prints, and use as needed..
i forget who I learned that from.. im sure I read it somewhere, most likely here.. i didnt invent that.. haha
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OregonChronic
Iron, Lion, Cyans



Registered: 09/12/10
Posts: 1,970
Loc: Scoping Woodchips
Last seen: 11 years, 7 days
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Ya this thread really helped me out im glad i posted it..i thought spore printing was a more complicated thing that mighta needed some pressure sterilization but i was wrong 
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Wavy Cap Cyan Spore Prints for Trade/Sale
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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nah.. honestly, its one of the easier procedures in this hobby..
the thing I see the most problems with people getting right is grain prep, but thats a whhooooolllleee different thread.. haha
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SomeGuy
I feel better now :)


Registered: 04/18/10
Posts: 7,496
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Last seen: 9 years, 8 months
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of course, sterilize your hands/scissors before cutting the stem off. I've had problems getting a print off a dead mush with no stem. That's why I do it the way I do
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OregonChronic
Iron, Lion, Cyans



Registered: 09/12/10
Posts: 1,970
Loc: Scoping Woodchips
Last seen: 11 years, 7 days
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Re: Spore Printing [Re: SomeGuy]
#14231244 - 04/03/11 01:49 PM (12 years, 9 months ago) |
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So exactly what kind of syringes should i be asking for at the pharmacy when i call to ask what they carry? My town has alot of bad drug use and they will most likely right away question me.
Just ask for a 10cc syringe with a needle?
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Wavy Cap Cyan Spore Prints for Trade/Sale
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Cynosure
allow me to be your guide.


Registered: 10/06/09
Posts: 4,228
Last seen: 11 months, 12 days
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I just asked for syringes, then they asked "how big" and I responded with 10ccs. They'll probably ask you what you are using them for, so you might want to come up with a decent excuse or explain that it's for mycology (depends on how paranoid you are). Then ask them for 18g needles to accompany them.
I don't think you can purchase just one.. I had to buy them in packs of 100.
-------------------- "You can peel it [language] off the ceiling and make it dance in front of you" - McKenna <3 .
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SomeGuy
I feel better now :)


Registered: 04/18/10
Posts: 7,496
Loc:
Last seen: 9 years, 8 months
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Go to Tractor supply, Quality farm & fleet or the animal feed store. Your vaccinating cows, after all
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Bacchus
Lurker




Registered: 10/10/06
Posts: 914
Loc: ::1
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If you have a science supply store in your area, you can try there. They won't ask you any questions. Pick up some petri dishes and parafilm while you're at it too
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Living on a no-Flash diet is way easier than you think. Give it a shot.
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deadmandave
Slime


Registered: 02/16/10
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Re: Spore Printing [Re: SomeGuy]
#14231312 - 04/03/11 02:09 PM (12 years, 9 months ago) |
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Quote:
I spray my hands with lysol.

I dont think lysol is very effective at sanitizing hands. 70% iso alcohol is cheap and does the trick.
As far as putting the whole cap in a shot glass and getting spores that way - this method works and is used by lots of commercial cultivators, however they grow the mushroom to be used in a sterile environment. You have to remember that the cap you are putting in water has been exposed to regular ambient air for ~1 week and is not sanitary at all. So all the contaminants that were on the top of the cap are now floating in your spore syringes.
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jokefox
Top of the chain



Registered: 12/22/09
Posts: 6,231
Loc: never where I should be
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Re: Spore Printing [Re: Bacchus]
#14231313 - 04/03/11 02:09 PM (12 years, 9 months ago) |
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put water in a jar in the pc, dont do that stove method,
better to do something right then half ass
if you need to sterlize syringes and tools, put those in a dry jar in the pc also ,
no foil, no bullshit, no chance for wrong just use a glove box also
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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Re: Spore Printing [Re: jokefox]
#14233656 - 04/03/11 09:54 PM (12 years, 9 months ago) |
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just order your syringes online from a vendor on here.. im sure they will be discrete, and will have what you need..
Quote:
You have to remember that the cap you are putting in water has been exposed to regular ambient air for ~1 week and is not sanitary at all. So all the contaminants that were on the top of the cap are now floating in your spore syringes.
thats only if your FC is not clean.. lol.. whats the difference between putting a cap down to make a print, as opposed to making a syringe? the cap, that has "been exposed", is still present during the printing process..
PS:
Quote:
You have to remember that the cap you are putting in water
no one ever mentioned a whole cap in water..
Edited by DJYoshaBYD (04/03/11 09:55 PM)
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