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Offlinegottabuzz84
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Registered: 10/26/10
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Don't know where i screwed up, advice urgently welcome!!!
    #14222800 - 04/01/11 07:22 PM (12 years, 9 months ago)

Thanks in advance for anyone who offers me a hand, and i have been going over post for the past 3 hours and cant figure this one out.     
 
    Trichoderma just beat the shit out of me and I lay humbled and confused for I dont know where I went wrong.

  I've been doing the PF tek for over a year, and for the last 6 month I have been experimenting with liquid cultures, with awesome results... Until this last time.

  I made a golden teacher LC and a shooting star LC, which looked beautiful in the end. I knocked up 24 jars of each strain out of each culture. The Golden Teacher batch was slow to show anything, and a week later the substrate was covered in trich with no mycelium.

  I came to the conclusion this was because my LC was contaminated and i couldnt tell while it was in the jar, which i hear its kinda tough to see contams in a LC until you use it.
 
  Okay, i wiped my tears and held hope for my shooting stars. A week or two after noccin them up they were looking ideal, the mycelium was so ryzomorphic and looked as nothing could stop it.

  So today, which was the end of week three, i check my jars and to my utter disbelief, GREEN MOLD! FUCK! excuse my language, it was for lack of a better word. I was able to salvage 3 or 4 jars out of the 24, so i tucked my tail and limped away.

  So my question to you, my senior mycologist, how did this happen? I keep my jars in an incubator, but i keep my temps at 78-80 degrees. My sterile technique is nothing short of paranoid. Now, a piece of info i just found out, my friends dog tripped the cord to my incubator, causing my temps to plummet to 68-70 degrees for a few days, could this be the problem? I appreciate anyone who reads this and adds input because i am scratching my head raw and bald. I've never been hurt by trich this bad and i stand humbled. Thanks again.


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Offlinesolumvita
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Re: Don't know where i screwed up, advice urgently welcome!!! [Re: gottabuzz84]
    #14225934 - 04/02/11 01:00 PM (12 years, 9 months ago)

sounds like your LC is contaminated


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Offlinegottabuzz84
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Re: Don't know where i screwed up, advice urgently welcome!!! [Re: solumvita]
    #14233590 - 04/03/11 09:43 PM (12 years, 9 months ago)

I know the GT LC was defently contaminated; but the shooting stars looked perfect for almost 3 weeks, i mean beautiful! But I'm leaning towards contamed LC too, except, everytime i've seen Trich, it was within a week, not after 75% colonization.

The LC in question was never refridgerated but in my incubator for 2 months or better,  think this has somthing to do with it? I never refridgerate my LC, but believe I'm gonna start after this!


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Invisibleanonjon
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Registered: 11/03/08
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Re: Don't know where i screwed up, advice urgently welcome!!! [Re: gottabuzz84]
    #14235686 - 04/04/11 11:25 AM (12 years, 9 months ago)

Yes that's too old.


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Offlinehappihourhero
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Re: Don't know where i screwed up, advice urgently welcome!!! [Re: anonjon]
    #14241644 - 04/05/11 12:24 PM (12 years, 9 months ago)

LC's shouldnt take 3 weeks to reach 75% colonization.

And you should have done test jars before going balls to the wall like that.


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Offlinegottabuzz84
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Re: Don't know where i screwed up, advice urgently welcome!!! [Re: happihourhero]
    #14242229 - 04/05/11 02:31 PM (12 years, 9 months ago)

I've only been in this hobby a little over a year so i still have LOTS to learn. This is the first time i've had trouble with any of my LC's, it was a big let down.
  I still cant believe my BRF cake were looking so good and then BOOM, contaminated. And yes, I will do test jars from now on. How long do yall let your LC's go before refridgeration or using as inoculate? I've read many things on many threads, so i'm just curious to how long you experienced growers wait. Thanks for everyones replies.


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OfflineHumility
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Re: Don't know where i screwed up, advice urgently welcome!!! [Re: gottabuzz84]
    #14271737 - 04/11/11 11:19 AM (12 years, 9 months ago)

Quote:

So my question to you, my senior mycologist, how did this happen? I keep my jars in an incubator, but i keep my temps at 78-80 degrees.




Too hot imo; 75 is usually better.

I hear incubators are asking for trouble.

Dog trips cord; low temps would never cause infection the same way high temps would.  Our fungus is more resilient to cold shock, far more, than bacteria or competing fungi, which usually operate best over 90+ degrees.

Quote:


The LC in question was never refridgerated but in my incubator for 2 months or better,  think this has somthing to do with it? I never refridgerate my LC, but believe I'm gonna start after this!




Well apparently you've been having success up until now so I don't know, but you should always refrigerate your LC.  It stunts growth and induces a stupor which elongates the life of the mycelium and greatly prohibits contaminant growth.

Better luck next time bro.  Sounds like you learned a cheap lesson, they are the best.


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Offlinegottabuzz84
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Re: Don't know where i screwed up, advice urgently welcome!!! [Re: Humility]
    #14275264 - 04/11/11 10:07 PM (12 years, 9 months ago)

Well I found out what happened... I should have known but it never occured to me til it was brought to my attention.
The reason for the incubator is because the location is a cold place during the winter, and old houses dont fight cold well. Therefore jars were placed in incubator kept at 78-80 degrees...

Quote:


"Dog trips cord; low temps would never cause infection the same way high temps would.  Our fungus is more resilient to cold shock, far more, than bacteria or competing fungi, which usually operate best over 90+ degrees"







  that is until outdoor temps rise up causing house to warm up, sending incubator into the 90's which in turn creates a hotbed for contams... Sucks, but atleast i know better now.

Thanks everybody for your input, and Humility, you nailed it on the head bro


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OfflineFrontchasack
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Re: Don't know where i screwed up, advice urgently welcome!!! [Re: gottabuzz84]
    #14275310 - 04/11/11 10:13 PM (12 years, 9 months ago)

It sounds to me like maybe you had a sterilization issue with your LC's. How are you sterilizing and what kind of medium are you using for LC? If your new to LC, I would use a baby bottle with the nipple flipped and filled with silicone for a self healing injector site. Also, I haven't ever tried to use dextrose or honey, I always have great results out of Karo.


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"I once was here, but now I'm not. I went away to smoke some pot. I left this here to prove a point, that life ain't shit without a joint."

R.I.P. J.M.B. 5.26.06


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Invisibleanonjon
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Registered: 11/03/08
Posts: 6,322
Re: Don't know where i screwed up, advice urgently welcome!!! [Re: Frontchasack]
    #14276632 - 04/12/11 05:49 AM (12 years, 9 months ago)

Karo is terrible compared to malt. Makes a big diff.


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The above post is fictional, hypothetical, or downright nonsensical.


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OfflineHumility
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Re: Don't know where i screwed up, advice urgently welcome!!! [Re: anonjon]
    #14276908 - 04/12/11 08:35 AM (12 years, 9 months ago)

Quote:

anonjon said:
Karo is terrible compared to malt. Makes a big diff.




I'll have to read to see if that holds water.  Something tells me nutrient solution is nutrient solution is nutrient solution.

Differences are extant but largely irrelevant IMO.  As of 2-3 days ago I just started my first jars of LC though so who am I to say.

I also used Karo so I'm a bit biased.  We'll see what happens.


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