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kataphrakt
Student

Registered: 03/22/11
Posts: 44
Last seen: 12 years, 2 months
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Pf tek - Very slow colonization [My first flush]
#14222366 - 04/01/11 05:50 PM (12 years, 9 months ago) |
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I inoculated 4 jars 8 days ago with 2.5 cc of the B+ strain in each jar. I'm using very fine verm and brf in the jars. They are being kept around 70-72 degrees F throughout the day. I gave them three days with no checking, but have been checking them every day since, expecting to see the first signs of mycelium. This is my first real go at shrooming, but I followed the pf tek write ups to a T. Since contamination isn't the problem yet, I'm concerned that I got a bunk syringe; to be honest I could barely see any spores in the syringe I got, but the site I got it from advertised dark ass syringes loaded with spores. Is the temp just too low? Any help would be appreciated.
Edited by kataphrakt (05/22/11 12:52 PM)
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HyperSpaceMaggot
Uncle Creepy


Registered: 03/31/11
Posts: 157
Last seen: 12 years, 9 months
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Re: Pf tek - Very slow colonization [Re: kataphrakt]
#14222370 - 04/01/11 05:51 PM (12 years, 9 months ago) |
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Temp is fine. Was the spore vendor a sponsor? Just give it some time.
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kataphrakt
Student

Registered: 03/22/11
Posts: 44
Last seen: 12 years, 2 months
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Yeah it was lil shop of spores.
-------------------- No glove, no love.
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: Pf tek - Very slow colonization [Re: kataphrakt]
#14222403 - 04/01/11 05:57 PM (12 years, 9 months ago) |
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Spore germination can take anywhere from 7-14 days to occur. Be patient young grasshopper.
Also 1CC is all that is needed per jar. 1/4 of a cc per injection point.
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Sarfix
Mycotic


Registered: 03/21/11
Posts: 107
Loc: Midwest
Last seen: 10 years, 7 months
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Do you have anything covering the top? ie: tape, foil, tyvek, or just dry verm? I was having close to the same problem. I'm on day 8 and it's been very slow so now I've allowed for better GE and that should help.
-------------------- Turn back Sarah. Turn back before it's too late. Tattoo Products for Sterility
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kataphrakt
Student

Registered: 03/22/11
Posts: 44
Last seen: 12 years, 2 months
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Thanks for the advice. The biggest lesson I've learned since I discovered the field of mycology is that it's not for the impatient.
Sarfix: I have foil covering the top, and they are in a loosely sealed tub (not air tight). GE could be the problem (or the lack there of).
-------------------- No glove, no love.
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: Pf tek - Very slow colonization [Re: kataphrakt]
#14222456 - 04/01/11 06:06 PM (12 years, 9 months ago) |
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The foil should be removed before inoculation and not placed back on the jars. I would remove it now on all your jars.
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Sarfix
Mycotic


Registered: 03/21/11
Posts: 107
Loc: Midwest
Last seen: 10 years, 7 months
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Re: Pf tek - Very slow colonization [Re: kataphrakt]
#14222468 - 04/01/11 06:08 PM (12 years, 9 months ago) |
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Well if you put the layer of dry verm in there while following the tek then you should just take the foil off and leave the tub unsealed. I don't know a whole lot, but that might really help. Keep me posted on any changes you make, and if they start growing. I started my first grow on the 23 so it sound's like we started the same day. I'd like to see how our experiences compare.
-------------------- Turn back Sarah. Turn back before it's too late. Tattoo Products for Sterility
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3Cajun1Mo8
Fung Padawan



Registered: 11/07/10
Posts: 698
Last seen: 16 days, 17 hours
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Re: Pf tek - Very slow colonization [Re: Sarfix]
#14223229 - 04/01/11 08:44 PM (12 years, 9 months ago) |
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I did some cakes with no dry verm layer (not recommended) with tape over nail sized holes my colonization was so slow, then I removed the tape and growth exploded and only 1 jar contam'd
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carnage11
Mushroom Magician


Registered: 04/01/11
Posts: 3,409
Loc: 407
Last seen: 7 years, 7 months
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Re: Pf tek - Very slow colonization [Re: kataphrakt]
#14224050 - 04/01/11 11:30 PM (12 years, 9 months ago) |
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Just wanted to say that I got my syringes from LSoS as well and it took my jars right around 5 days to start to show signs of myc. You could definitely see little spores in the syringe but they were not dark syringes. Just little flecks of black when held up to the light in clear liquid.
I have a dry layer of verm on top and I put the foil back on top of the jars when I was done inoculating. I also have them in a container with the lid on and a towel over that, inside a closet. The temp is room temp, around 77 degrees. Roughly 2 weeks in and they are close to full colonization as of today.
-------------------- You're breathing so I guess you're still alive Even if signs seem to tell me otherwise.
Edited by carnage11 (04/01/11 11:31 PM)
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kataphrakt
Student

Registered: 03/22/11
Posts: 44
Last seen: 12 years, 2 months
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Re: Pf tek - Very slow colonization [Re: 3Cajun1Mo8]
#14224067 - 04/01/11 11:34 PM (12 years, 9 months ago) |
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Just removed the foil, even set the lid of the tub ajar. Hopefully GE was the problem and now they'll jumpstart. I'll add pics if they colonize.
-------------------- No glove, no love.
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: Pf tek - Very slow colonization [Re: kataphrakt]
#14225001 - 04/02/11 07:50 AM (12 years, 9 months ago) |
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There is no problem from the sounds of it, spores take time to germinate, be patient and good things will come.
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kataphrakt
Student

Registered: 03/22/11
Posts: 44
Last seen: 12 years, 2 months
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Still nothing. Not that I want to throw my jars out, but at what point will I know it's a done deal?
-------------------- No glove, no love.
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darkhawk37
killzor

Registered: 03/17/11
Posts: 93
Last seen: 2 years, 3 months
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Re: Pf tek - Very slow colonization [Re: kataphrakt]
#14240998 - 04/05/11 10:10 AM (12 years, 9 months ago) |
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did u shake your syringe before u nocked them? it took like 2and a half weeks for the one b+ jar i got
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shroomiin
unprofound


Registered: 12/24/07
Posts: 2,470
Loc: Zone 6
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Re: Pf tek - Very slow colonization [Re: darkhawk37]
#14241110 - 04/05/11 10:39 AM (12 years, 9 months ago) |
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it needs more time dude i usually dont see any signes of myc for 2 weeks + when using a syringe. Especially when temps arnt ideal. Use lc for FAST colonization
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Mathna
Stranger



Registered: 01/07/10
Posts: 97
Loc: Stormwind, Eastern Kingdo...
Last seen: 9 years, 1 month
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Re: Pf tek - Very slow colonization [Re: shroomiin]
#14241130 - 04/05/11 10:44 AM (12 years, 9 months ago) |
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I use the strain of alacabanzi and they are on the longer end of the spectrum to show growth when using a spore syringe. LC is much faster, you should have made a LC with a portion of your syringe.
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kataphrakt
Student

Registered: 03/22/11
Posts: 44
Last seen: 12 years, 2 months
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Re: Pf tek - Very slow colonization [Re: Mathna]
#14242565 - 04/05/11 03:43 PM (12 years, 9 months ago) |
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Good news! Three jars have a tiny patch of myc now! And yes I shook the syringe. I also got a second syringe for an LC, but haven't made it yet. Thanks for the words of patience, guys
-------------------- No glove, no love.
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kataphrakt
Student

Registered: 03/22/11
Posts: 44
Last seen: 12 years, 2 months
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Re: Pf tek - Very slow colonization [Re: kataphrakt]
#14432669 - 05/10/11 11:29 PM (12 years, 8 months ago) |
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Just thought I'd follow up with my progress in this thread so I wouldn't have to start a new one. Just birthed, dunked, and rolled the 4 cakes. When they start pinning I'll post pics.
-------------------- No glove, no love.
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kataphrakt
Student

Registered: 03/22/11
Posts: 44
Last seen: 12 years, 2 months
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Re: Pf tek - Very slow colonization [Re: kataphrakt]
#14473389 - 05/18/11 01:42 PM (12 years, 8 months ago) |
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Bump for pins/ my first flush ever.

 Noticed the first ones 72 hours ago, one more 48 hours ago, now 10 total about 12 hours ago. Two cakes haven't even pinned at all, while the other two seem to be getting decent sets. Sorry for the horrible quality... my fancy iphone busted, so I had to revert to my old phone.
-------------------- No glove, no love.
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kataphrakt
Student

Registered: 03/22/11
Posts: 44
Last seen: 12 years, 2 months
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Re: Pf tek - Very slow colonization [Re: kataphrakt]
#14493917 - 05/22/11 12:45 PM (12 years, 8 months ago) |
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updated with more pins, better pics


 The better pinsets belong to the two Argentina cakes. The weakling is B+. The other B+ cake isn't even pinning... should I toss it?
-------------------- No glove, no love.
Edited by kataphrakt (05/22/11 12:46 PM)
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explosivekush
Stranger


Registered: 04/30/11
Posts: 86
Last seen: 10 years, 11 months
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Re: Pf tek - Very slow colonization [Re: kataphrakt]
#14494266 - 05/22/11 02:09 PM (12 years, 8 months ago) |
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nice job. as for the cake that isn't pinning dont toss it some just take longer then others.
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kataphrakt
Student

Registered: 03/22/11
Posts: 44
Last seen: 12 years, 2 months
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Re: Pf tek - Very slow colonization [Re: explosivekush]
#14503779 - 05/24/11 11:02 AM (12 years, 8 months ago) |
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Ta da! I harvested one cake because the fruits were beginning to slump into the perlite. The cake that hadn't started pinning just got some pins when I checked it this morning. Here's my first harvest! Still 3 cakes to harvest and hopefully I'll get multiple flushes. Also, would anyone recommend using rice to dry my fruits?
-------------------- No glove, no love.
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kataphrakt
Student


Registered: 03/22/11
Posts: 44
Last seen: 12 years, 2 months
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Re: Pf tek - Very slow colonization [Re: kataphrakt]
#14510014 - 05/25/11 01:58 PM (12 years, 8 months ago) |
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So, I ate 2g (dry) and didn't trip at all. The last time I ate shrooms was two years ago and I ate an eigth (wet) and tripped balls. Why the fuck aren't my shrooms potent.
-------------------- No glove, no love.
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creationdivine
Seeker Seeker



Registered: 07/23/09
Posts: 461
Loc: blest coast
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Re: Pf tek - Very slow colonization [Re: kataphrakt]
#14510049 - 05/25/11 02:05 PM (12 years, 8 months ago) |
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don't be angry man. just eat more. beautiful grow regardless.
--------------------
Kickin' it with some Cinctulus!
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shroomiin
unprofound


Registered: 12/24/07
Posts: 2,470
Loc: Zone 6
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an eighth wet is 35 grams. Your saying you ate 3.5 grams wet, or 35 grams wet? Becuase i call bullshit if you at 3.5 wet and tripped (.35 dry).
Are you positive you dried them all the way? Lots of times they seem dry until you put them in a bag and there is still moisture in there.
PLUS you harvested those early. Those little guys would weigh about .1 or .2 once dry. I would estimate the small pile in the last pic you posted to weigh about ~1g if dried properly.
Plus, if you leave them out too long while still wet the water content will destroy the psilocibin. How did you dry them?
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kataphrakt
Student


Registered: 03/22/11
Posts: 44
Last seen: 12 years, 2 months
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Eat more isn't an option now... I just redunked the cakes and they're fruiting again. I'm going to double the dose (if they flush that much)next time.
EDIT: The first time I ate an eight (3.5g is an 1/8 of an ounce), they were wet and from the field. I tripped balls, call bullshit if you want but it's the truth. Clear visuals and everything. Also, the previous picture is not all the shrooms I ate. I dried them with desiccant. I know what I'm doing, and I know those things had 0 potency. Also, there were at least 20 aborts that I ate aside from the big shrooms. Aren't they supposed to be teaming with alkaloids?
-------------------- No glove, no love.
Edited by kataphrakt (05/25/11 03:22 PM)
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