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Mycowlogist
Freethinker



Registered: 06/29/08
Posts: 534
Loc: in a galaxy far far away....
Last seen: 10 years, 1 month
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Sclerotia and air exchange
#14219968 - 04/01/11 10:35 AM (12 years, 10 months ago) |
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I am interested in growing only Sclerotia. I will not try to fruit the species. I have selected Galindoi/ATL#7.
I will be using 1 quart jars. Rye Berries (coffee, gypsum)
I will be inoculating with an spore syringe.
I want to use a plastic canning jar lid. I will be drilling a hole to glue in an injection port (got some really cheap) I also bought some Synthetic Filter Discs from The Mad Season. I plan on drilling a hole into the cap and cutting a piece of the filter to fit on top of the lid for air exchange.
My question. What diameter hole do you think I should create for optimal sclerotia production? I plan on letting the goodies stay in the jar for up to 6 Months.
Does any one have the stones to answer that question?
-------------------- "And in the end, it's not the years in your life that count. It's the life in your years." Honest Abe
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Sclerotia and air exchange [Re: Mycowlogist]
#14219987 - 04/01/11 10:39 AM (12 years, 10 months ago) |
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I've always used 1/4" holes. Can't really say if that's optimal but it works. I can say polyfil is an easy way to make a filter.
-------------------- You make it all possible. Doesn't it feel good?
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biologys
Mycologist in Trainning




Registered: 12/21/09
Posts: 4,622
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Re: Sclerotia and air exchange [Re: Mycowlogist]
#14219989 - 04/01/11 10:40 AM (12 years, 10 months ago) |
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I normally just use a 1/4 inch hole for GE.
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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Re: Sclerotia and air exchange [Re: biologys]
#14220063 - 04/01/11 10:58 AM (12 years, 10 months ago) |
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+1 for 1/4"
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: Sclerotia and air exchange [Re: biologys]
#14220092 - 04/01/11 11:03 AM (12 years, 10 months ago) |
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Lid tek in signature...I use 3/8" but have never done stones.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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Mycowlogist
Freethinker



Registered: 06/29/08
Posts: 534
Loc: in a galaxy far far away....
Last seen: 10 years, 1 month
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Alright 1/4" Seems to win it so far. Any one try a larger size to help improve oxygen exchange? Or would it dry it too quickly.
Thanks Todlow...love me some chowder.
I know of polyfill, I just prefer not to use it. Honestly I am lazy and I rather go through a more complex, and time consuming method than manually re-stuff the hole. Okay I am possibly lazy and stupid, but I am also a geek at heart and love modifying things.
Any other stoners out there?..... Sclerotia I mean
-------------------- "And in the end, it's not the years in your life that count. It's the life in your years." Honest Abe
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Sclerotia and air exchange [Re: Mycowlogist]
#14220387 - 04/01/11 11:56 AM (12 years, 10 months ago) |
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I don't restuff the polyfil unless I need to.
I've got some ATL 7 spawned to wet verm in a tub right now.
-------------------- You make it all possible. Doesn't it feel good?
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Mycowlogist
Freethinker



Registered: 06/29/08
Posts: 534
Loc: in a galaxy far far away....
Last seen: 10 years, 1 month
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Re: Sclerotia and air exchange [Re: Doc_T]
#14220430 - 04/01/11 12:05 PM (12 years, 10 months ago) |
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One day... Soon....
I have read a couple of your posts... Helped a lot in my decision making. Plus the picture distracts me. That is a cutey
-------------------- "And in the end, it's not the years in your life that count. It's the life in your years." Honest Abe
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Sillicybin
Registered: 02/14/05
Posts: 2,134
Loc:
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Re: Sclerotia and air exchange [Re: Mycowlogist]
#14220757 - 04/01/11 12:56 PM (12 years, 10 months ago) |
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Quote:
Mycowlogist said: Okay I am possibly lazy and stupid, but I am also a geek at heart and love modifying things.
Nah, I feel the same. It's not lazy/stupid though, it's just that I feel that polyfil is kinda ghetto. Yes it works, but it's a pain in the ass and it's ugly.
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Smoke McWeedson


Registered: 02/10/10
Posts: 123
Last seen: 7 days, 18 hours
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Re: Sclerotia and air exchange [Re: Mycowlogist]
#14220771 - 04/01/11 12:58 PM (12 years, 10 months ago) |
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1/4 inch is what i use as well
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Sclerotia and air exchange [Re: Sillicybin]
#14220779 - 04/01/11 12:59 PM (12 years, 10 months ago) |
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I think polyfil is cute and fuzzy. 
But I'd use synthetic discs if appearance mattered much to me. Tyvek is pretty but doesn't work well.
-------------------- You make it all possible. Doesn't it feel good?
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Sillicybin
Registered: 02/14/05
Posts: 2,134
Loc:
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Re: Sclerotia and air exchange [Re: Doc_T]
#14220818 - 04/01/11 01:09 PM (12 years, 10 months ago) |
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Quote:
Doc_T said: But I'd use synthetic discs if appearance mattered much to me. Tyvek is pretty but doesn't work well.
BTW, I'm not knocking anyone. My main beef with polyfil is the inability to stack jars well during colonization, but the same goes for injection ports and Whatman filters.
Doc, you and RR both dislike Tyvek (with RR, he especially hates P.O. Tyvek) but good ol' USPS standard with a glob of RTV (still ghetto for my taste, I need to get some filter discs) and polyfil is all I've ever used for filters. I've never had any problems with the Tyvek, except for when I tried to use a single layer as a complete replacement for the lid and just put that under the band. The problem with that was not contamination, the grain just dried out before colonization completed. Trying again, I was able to solve that by just using two layers sandwiched.
What's your experience with Tyvek? Where does it seem to fail?
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Sclerotia and air exchange [Re: Sillicybin]
#14220829 - 04/01/11 01:11 PM (12 years, 10 months ago) |
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It's not durable over time. It degrades every time you PC it until it's useless. And people stealing it just irks me.
I don't stack jars directly, I always use the flats they come in. I'd be knocking some jars over if I stacked them.
-------------------- You make it all possible. Doesn't it feel good?
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Sillicybin
Registered: 02/14/05
Posts: 2,134
Loc:
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Re: Sclerotia and air exchange [Re: Doc_T]
#14221043 - 04/01/11 01:42 PM (12 years, 10 months ago) |
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Quote:
Doc_T said: It's not durable over time. It degrades every time you PC it until it's useless. And people stealing it just irks me.
Yeah, I can understand that. I did ask if I could pay for it once, but said they didn't have any way to ring it up. I don't ever reuse Tyvek for that reason, though - it does warp slightly when pressure cooked.
I can do 8 widemouth jars with one envelope. I usually do 12 jars every 6 months or so (one grow lasts me a long time), so I end up using 3-4 envelopes a year. I justify that to myself by never wasting an envelope and I still mail packages via USPS when UPS would be faster and cheaper.
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anonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
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Re: Sclerotia and air exchange [Re: Sillicybin]
#14221153 - 04/01/11 02:01 PM (12 years, 10 months ago) |
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I think polyfill is cute and fuzzy too. Not to mention effective and cheap.
-------------------- The above post is fictional, hypothetical, or downright nonsensical.
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Sclerotia and air exchange [Re: Sillicybin]
#14221600 - 04/01/11 03:18 PM (12 years, 10 months ago) |
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USPS is usually my best choice, I wonder why we differ on shipping? 
You can buy better thicker more reusable tyvek from kite shops, but it still doesn't last as long as a filter disc or polyfil.
-------------------- You make it all possible. Doesn't it feel good?
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