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decafosmi
student



Registered: 11/02/10
Posts: 6
Loc: MFUSA
Last seen: 12 years, 8 months
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about to do a g2g... anyone have anyone helpful info
#14216954 - 03/31/11 07:56 PM (12 years, 9 months ago) |
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I have some treasure coast #3 that I have been playing with. When I cased my other bags I did not have enough vermiculite to case the last one, so it has been sitting in the incubator for a weeks weeks untouched. I just sterilized 6 1 1/2 quarter bags and have let them cool so that I can do the grain to grain transfer. I do not have a glove box but I have a semi sterile grow room that I keep my garden in. Does anyone have any info that will help me with this process so that I can stay contamination free???
-------------------- yummy yummy mushroom tea some for you some for me
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: about to do a g2g... anyone have anyone helpful info [Re: decafosmi]
#14216960 - 03/31/11 07:57 PM (12 years, 9 months ago) |
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Take a shower.
-------------------- You make it all possible. Doesn't it feel good?
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decafosmi
student



Registered: 11/02/10
Posts: 6
Loc: MFUSA
Last seen: 12 years, 8 months
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Re: about to do a g2g... anyone have anyone helpful info [Re: Doc_T]
#14216985 - 03/31/11 08:02 PM (12 years, 9 months ago) |
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another question... would it be safe to make a spore syringe from the bag before I do the transfer?
-------------------- yummy yummy mushroom tea some for you some for me
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: about to do a g2g... anyone have anyone helpful info [Re: decafosmi]
#14216996 - 03/31/11 08:05 PM (12 years, 9 months ago) |
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You've got an open cap in the bag?
-------------------- You make it all possible. Doesn't it feel good?
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decafosmi
student



Registered: 11/02/10
Posts: 6
Loc: MFUSA
Last seen: 12 years, 8 months
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Re: about to do a g2g... anyone have anyone helpful info [Re: Doc_T]
#14217009 - 03/31/11 08:07 PM (12 years, 9 months ago) |
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not sure what you mean by an open cap. It is just a grow bag with a filter patch. Can you please explain? ahhhh I got it now no I do not
-------------------- yummy yummy mushroom tea some for you some for me
Edited by decafosmi (03/31/11 08:08 PM)
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mathias
Ersatz Haderach



Registered: 05/01/10
Posts: 561
Last seen: 12 years, 1 month
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Re: about to do a g2g... anyone have anyone helpful info [Re: decafosmi]
#14217076 - 03/31/11 08:25 PM (12 years, 9 months ago) |
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Taken from colonized subs, you won't have a "spore" syringe, but rather a syringe of water and mycelium. Done clean, this can be very useful. Illegal to ship, but handy.
Spore syringes are made from prints or otherwise collected spores. Legal to ship since they have no illegal compounds.
-------------------- intlslacker : "Why is it I always have the feeling that if RR weren't around it'd be like there were toddlers running around, bumping into walls and eating poisonous mushrooms?"
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decafosmi
student



Registered: 11/02/10
Posts: 6
Loc: MFUSA
Last seen: 12 years, 8 months
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Re: about to do a g2g... anyone have anyone helpful info [Re: mathias]
#14217115 - 03/31/11 08:34 PM (12 years, 9 months ago) |
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so if I was just to stick a syringe into the bag and draw up the yellow colored water in the bottom of the bag would I be able to innoc an LC with that or what kinda mixture should I use to get that syringe to expand my growth
-------------------- yummy yummy mushroom tea some for you some for me
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: about to do a g2g... anyone have anyone helpful info [Re: decafosmi]
#14217169 - 03/31/11 08:45 PM (12 years, 9 months ago) |
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EEeeewww, no. Post pics.
-------------------- You make it all possible. Doesn't it feel good?
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decafosmi
student



Registered: 11/02/10
Posts: 6
Loc: MFUSA
Last seen: 12 years, 8 months
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Re: about to do a g2g... anyone have anyone helpful info [Re: Doc_T]
#14217233 - 03/31/11 08:56 PM (12 years, 9 months ago) |
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-------------------- yummy yummy mushroom tea some for you some for me
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mathias
Ersatz Haderach



Registered: 05/01/10
Posts: 561
Last seen: 12 years, 1 month
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Re: about to do a g2g... anyone have anyone helpful info [Re: decafosmi]
#14222185 - 04/01/11 05:11 PM (12 years, 9 months ago) |
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Quote:
decafosmi said: so if I was just to stick a syringe into the bag and draw up the yellow colored water in the bottom of the bag would I be able to innoc an LC with that or what kinda mixture should I use to get that syringe to expand my growth
If I understand you correctly, no.
The moisture in the bag is not what you want to collect. You need to inject sterile water into the jar/bag, break apart a small amount of mycelium with the tip of the needle and suck it back into the syringe. This can then be used to start an LC or other jars/bags.
Advantages: Done right, one little jar or bag can spawn an army. Syringes can be stored a long time in a cool place. Some prefer this tek over spore collecting or agar work. Not terribly difficult or expensive. With perfect sterility and a good isolate, this tek can rock. Disadvantages: Risky. One error in sterile tek can ruin both sample and source. While not "difficult", this tek has to be done RIGHT. Syringes cannot be shipped; mycelium contains illegal compounds. Unless taken from a proven isolated strain, this tek is can be even more of a crap-shoot than starting fresh from multi-spore, as far as what genetics you are actually harvesting and growing.
Results from MS sources are unpredictable. If you happen to suck up mostly non-fruiting strains, well, there you go. Wait for the whole thing to become a single mass (but NOT pin at all yet) and get a good sample from a pea-sized area. This helps ensure that you get a good sample of all the vectors you've got. Bone up on "anastomosis" to understand how mushroom genetics works.
Many become one. One is all. We are Legion.
I've had better results doing G2G in jars by hand; glovebox and all that. 4 dime size chunks of spawn per jar do well.
-------------------- intlslacker : "Why is it I always have the feeling that if RR weren't around it'd be like there were toddlers running around, bumping into walls and eating poisonous mushrooms?"
Edited by mathias (04/02/11 10:49 AM)
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