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OfflineMycowlogist
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Using micro/trace elements for grain preparation
    #14216693 - 03/31/11 07:18 PM (12 years, 9 months ago)

I have been reading about how using coffee water to soak your rye grain is a good way to expedite mycelial growth.  Is it the nitrogen in the coffee?  Or is it other nutrients along with the pH drop, which help in the process. 

I have been growing aquarium plants as a hobby for the past 5 years and understand how important Trace Elements, and Micro-nutrients are for proper development.  Would using something like Seachem Flourish which contains:

Total Nitrogen
0.07%
Available Phosphate ( P2O5)
0.01%
Soluble Potash
0.37%
Calcium (Ca)
0.14%
Magnesium (Mg)
0.11%
Sulfur (S)
0.2773%
Boron (B)
0.009%
Chlorine (Cl)
1.15%
Cobalt (Co)
0.0004%
Copper (Cu)
0.0001%
Iron (Fe)
0.32%
Manganese (Mn)
0.0118%
Molybdenum (Mo)
0.0009%
Sodium (Na)
0.13%
Zinc (Zn)
0.0007%


Provide a better solution to prepare your grains in?  I feel we are missing an aspect of this process by ignoring additional nutrients which may help improve our methods. 

I am interested in your feedback?


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"And in the end, it's not the years in your life that count. It's the life in your years."  Honest Abe


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OfflineRogerRabbitM
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Re: Using micro/trace elements for grain preparation [Re: Mycowlogist]
    #14217567 - 03/31/11 09:50 PM (12 years, 9 months ago)

I doubt seriously it's the N in coffee that provides the boost.  I suspect the lower pH reduces colonization time.  Most of the minerals mushrooms require are in the substrates and/or water we use.  We add gypsum as a mineral source, but I doubt you'd see much benefit from hydroponic micro.  If you have some, give it a try.  I doubt it could hurt anything.
RR


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OfflineMycowlogist
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Re: Using micro/trace elements for grain preparation [Re: RogerRabbit]
    #14217951 - 03/31/11 10:59 PM (12 years, 9 months ago)

I was thinking that having additional Iron supplement would provide better oxygen transfer, which may help expedite a faster colonization thus decreasing the chance for contams to come in the picture.  Iron attracts oxygen kind like in our lungs attracting oxygen into our blood, or how it ionizes with oxygen creating Iron Oxide (rust).  Mushrooms are high in Iron as a food source, which I would think they would get that from their natural substrate.  Also if it's the pH.  Could we try to get it lowered in the Rye mixture to adjust to the preferred pH for the fungi?  It's fun to think outside the "glovebox"


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"And in the end, it's not the years in your life that count. It's the life in your years."  Honest Abe


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InvisibleCheeWiz
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Registered: 09/28/08
Posts: 276
Re: Using micro/trace elements for grain preparation [Re: Mycowlogist]
    #14218708 - 04/01/11 02:31 AM (12 years, 9 months ago)

Hi; adding micro and trace elements to spawn grain is a bit of a waste. The grain itself is almost the perfect base for spawn to grow on and already has all nutrients needed for good growth on its own. As RR stated it's the lowering of the PH that's a help in reducing colonization times. In turn when you add the spawn to your bulk substrate the growing mycelium will get all the required micro and trace elements it needs from that substrate.

There are things that can help speed up the mycelium growth on a spawn grain that include lowering the PH and oxygen spiking. The lowering of the PH can be done with everything from coffee, sour salt (citrate acid) to succinic acid. This works well for agars, LC and spawn grains, but you’re asking for contaminant problems with a bulk substrate if the PH is to low and it’s better to keep it up around a PH of 8 for that reason. As for oxygen spiking we are still doing test with it, but it looks good. There are two things that I have been working on and that's a guild to making mediums and oxygen spiking. Things are a little slow at my end with my caring for my mother and travel to Southeast Asia and China. But we all have to pay our bills.


Edited by CheeWiz (04/01/11 09:39 AM)


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OfflineMycowlogist
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Re: Using micro/trace elements for grain preparation [Re: CheeWiz]
    #14219126 - 04/01/11 05:24 AM (12 years, 9 months ago)

Thanks for both of your feedback.  I still may try it to test it out.  But feel confident that you guys are going to be right.  still a jar or two that gets tested won't hurt nothing.  I will post if I find anything interesting.


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"And in the end, it's not the years in your life that count. It's the life in your years."  Honest Abe


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