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OfflineMedici_head
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BRF Cakes: variation with inoculum injections?
    #14216104 - 03/31/11 05:39 PM (12 years, 9 months ago)

Someone told me that approximately 1/2-mL worth of syringe spore solution would be needed per inoculation port, totaling 2-mL.

Would there be any particular advantage of injecting more/less spores than this quantity per jar? 

Would it be wasteful? 

More spores per jar = more chance of survival per jar?

Cheers, Medici



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Y o u ' r e  a l l  G o d s ;  Y o u ' r e  a l l  G o d s ;  Y o u ' r e  a l l  2  m i l l i o n  y e a r s  o l d . ~T. Leary


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OfflinePsilocyentist
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Re: BRF Cakes: variation with inoculum injections? [Re: Medici_head]
    #14216113 - 03/31/11 05:40 PM (12 years, 9 months ago)

More spores does not equal better results/better chance of survival. Using 2ccs per jar is wasteful. 1CC per jar is the standard.


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OfflinePatlal
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Re: BRF Cakes: variation with inoculum injections? [Re: Psilocyentist]
    #14216127 - 03/31/11 05:42 PM (12 years, 9 months ago)

Quote:

Psilocyentist said:
More spores does not equal better results/better chance of survival. Using 2ccs per jar is wasteful. 1CC per jar is the standard.




:whathesaid:


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InvisibleDoc_T
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Re: BRF Cakes: variation with inoculum injections? [Re: Medici_head]
    #14216150 - 03/31/11 05:45 PM (12 years, 9 months ago)

Less is more with spores. Use a tiny dribble, just enough to see it trickle down the glass.


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Offlinedr0409
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Re: BRF Cakes: variation with inoculum injections? [Re: Patlal]
    #14216157 - 03/31/11 05:47 PM (12 years, 9 months ago)

You can get away with using less than 1cc per jar if ya want.  More spores dose not equal better.


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OfflineSomeGuy
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Re: BRF Cakes: variation with inoculum injections? [Re: dr0409]
    #14216185 - 03/31/11 05:51 PM (12 years, 9 months ago)

I will say this....On my pint pf jars, 1 cc germinates deeper in the jar than 1/2 cc. I reckon it runs down the glass further before being absorbed by the verm. This is the one instance where more is more, but diluting the solution isn't a bad idea


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OfflineMedici_head
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Re: BRF Cakes: variation with inoculum injections? [Re: dr0409]
    #14216227 - 03/31/11 05:56 PM (12 years, 9 months ago)

Thanks everyone for your responses.  Smaller portions must be better!

EDIT: I also agree with paducahovoids though, more could mean more surface area covered initially, like stabilizing the surface of the cake rather quickly.. hmm


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Y o u ' r e  a l l  G o d s ;  Y o u ' r e  a l l  G o d s ;  Y o u ' r e  a l l  2  m i l l i o n  y e a r s  o l d . ~T. Leary


Edited by Medici_head (03/31/11 06:00 PM)


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InvisibleDoc_T
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Re: BRF Cakes: variation with inoculum injections? [Re: Medici_head]
    #14216528 - 03/31/11 06:53 PM (12 years, 9 months ago)

No. Don't over shoot.


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You make it all possible. Doesn't it feel good?


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