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OfflineTerry M
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Registered: 06/18/10
Posts: 1,502
Loc: Rhode Island
Last seen: 9 years, 1 month
Grabbing spawn from PF cakes
    #14214061 - 03/31/11 10:06 AM (12 years, 10 months ago)

I've got some cakes growing out and nearly 100% colonized, and somewhere during the birthing process I'd like to grab some uncontaminated or at least minimally contaminated spawn to grow on agar as P1s. Does anybody have experience doing this? I've grabbed colonized grain kernels from jars during the process of inoculating bags, and have got some great, pure cultures this way by dropping a single grain kernel onto the center of a plate.


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Liberté, égalité, humidité.

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OfflinePatlal
You ask too many questions
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Registered: 10/09/10
Posts: 44,818
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Re: Grabbing spawn from PF cakes [Re: Terry M]
    #14214078 - 03/31/11 10:10 AM (12 years, 10 months ago)

Well, be sure to do it in a sterile, contam free area.

Simply take a small piece of mycellium and put in on your agar.

Everything should work fine!

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OfflineMaharishi_2_U
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Registered: 10/21/09
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Re: Grabbing spawn from PF cakes [Re: Patlal]
    #14214088 - 03/31/11 10:14 AM (12 years, 10 months ago)

Quote:

Patlal said:
Well, be sure to do it in a sterile, contam free area.

Simply take a small piece of mycellium and put in on your agar.

Everything should work fine!



:whathesaid:

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Invisibleanonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
Re: Grabbing spawn from PF cakes [Re: Maharishi_2_U]
    #14214096 - 03/31/11 10:17 AM (12 years, 10 months ago)

I've just ripped off a chunk and dipped it in peroxide 10 seconds and dropped it in there.

I got a bad syringe so I just found a good spot on one of the contaminated jars and went to agar.

If the chunk is big enuff it will even fruit in the agar jar.


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The above post is fictional, hypothetical, or downright nonsensical.


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OfflineTerry M
Stranger in a Strange Land
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Registered: 06/18/10
Posts: 1,502
Loc: Rhode Island
Last seen: 9 years, 1 month
Re: Grabbing spawn from PF cakes [Re: Patlal]
    #14214101 - 03/31/11 10:19 AM (12 years, 10 months ago)

Quote:

Patlal said:
Well, be sure to do it in a sterile, contam free area.

Simply take a small piece of mycellium and put in on your agar.

Everything should work fine!



Great, thanks! I was hoping it was that simple, but didn't want to assume anything.


--------------------
Liberté, égalité, humidité.

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