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Invisiblehamloaf
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Grain to Grain Transfer From Spawn Bag to Quart Jar.
    #14209841 - 03/30/11 02:23 PM (12 years, 10 months ago)

Any knowledge, advice, useful tips before I begin?  Thanks, have a great day and as always, Happy Shrooming!


:vaped:


Update: 4-16-11


Transferring fully colonized grains from bag to jar was a piece of cake and went a lot soother than anticipated. 


Using the 5 quart bag of fully colonized grains (WBS), was able to inoculate all 27 horse manure+ tea jars,


 





with this much grains left over.








With the 9 quart spawn bag, 45 jars were able to be inoculated,





With this much grains left over.








Let's see what culminates.  :popcorn:


Update, 4-21-11


So far, so good. 





Bout to shake in a few.


Update: 4/23,


Jars are recovering nicely after just 1-2 days from the mycelium being shook apart.  Not all of the jars were shook at the same time due to time constraints but were shook within 6 hours from the time the first round of jars were shook. 








While the jars were being sorted through, 2 of 72 jars, thus far have become contaminated with 2 separate kinds of bacteria. 


One was cob-web mold.





The other of coarse, trichoderma. 








These last three jars were found to be well over 95% colonization so the decision was made to not shake these jars. 





Update,  4-28-11



Almost there.......... (at 100% colonization). 





Rye suppliers harvested.  Got 400 pounds of it (rye #1), for 70 bux. 





Like how clean, freshly harvested rye can be from time to time.  This is the rye just after filling the pot with water and then stirring for the first time, no rinse yet. 





Hell yeah.  This rye only took one rinse with hot water and stirred before it was clean enough to add gypsum, beginning the soak duration.  That's going to considerably save some time spent rinsing the rye. 





Time to get to work prepping the grains to be loaded into bags and then sterilized.  More to come.  :popcorn:



update 5-3-11


Been there for a few days now (at 100% colonization).





Started the bags last weeks but due to life happening, had to halt production.


Between 2 25x electric AA steroclaves and an AA, model 941 PC, 14, 6 quart spawn bags filled with properly prepared rye were able to be sterilized at once.


The sterile bags were removed from the PC, wiped with an alcohol soaked paper towel and placed into a few large storage totes that were also wiped down with an alcohol soaked paper towel to safely store the sterile bags while production continues.


update 5-3-11


Been there for a few days now (at 100% colonization).





Started the bags last weeks but due to life happening, had to halt production.


Between 2 25x electric AA steroclaves and an AA, model 941 PC, 14, 6 quart spawn bags filled with properly prepared rye were able to be sterilized at once.


The sterile bags were removed from the PC, wiped with an alcohol soaked paper towel and placed into a few large storage totes that were also wiped down with an alcohol soaked paper towel to safely store the sterile bags while production continues.





My region's climate is going to be experiencing hot dry weather for weeks to come.  It's about time to spread the horse manure over a tarp and begin shredding and further dehydrating it out in that hot sun.


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Edited by liquidmyce (05/03/11 10:35 PM)

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Offlinemabachko
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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14209876 - 03/30/11 02:30 PM (12 years, 10 months ago)

I just did that the other day and using a GB is a PITA but it can be done
I only used 5 qrt jars for the 1 pound of rye bag that was colonized. I also had 7 1/2 pints that I wanted to try a G2BRF. That was probably why it was a PITA. Not much room to work in.
But all went well.
I wipe the bag and all other items down with alcohol prior to putting them into the GB, then sprayed Lysol & closed the lid. Waited about 10 minutes & started the deed.
So far no contams.
Good Luck

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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: mabachko]
    #14209972 - 03/30/11 02:45 PM (12 years, 10 months ago)

Quote:

mabachko said:
I just did that the other day and using a GB is a PITA but it can be done
I only used 5 qrt jars for the 1 pound of rye bag that was colonized. I also had 7 1/2 pints that I wanted to try a G2BRF. That was probably why it was a PITA. Not much room to work in.
But all went well.
I wipe the bag and all other items down with alcohol prior to putting them into the GB, then sprayed Lysol & closed the lid. Waited about 10 minutes & started the deed.
So far no contams.
Good Luck



Sounds good, my man.  I hope all works out well for you.  :mushroom2:


Thanks for the advise.  Will be working with 5 pound bag of fully colonized WBS and I have a flowhood.  That's a good idea, wiping all cultivation tools and items with an alcohol soaked paper towel before I move them in front of the flowhood or inside of a still air box and is part of my normal sterile procedure.


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14210033 - 03/30/11 03:02 PM (12 years, 10 months ago)

Never done G2BRF. Keep us posted. May the shrooms smile upon your endeavor.

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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: skyjohnny]
    #14210120 - 03/30/11 03:27 PM (12 years, 10 months ago)

Posted a pic last night.



But it has doubled in growth since then.
Was told it was a nono so I started it to see what happens.
Will update as time goes on.

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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14210163 - 03/30/11 03:36 PM (12 years, 10 months ago)

Quote:

hamloaf said:
Any knowledge, advice, useful tips before I begin?  Thanks, have a great day and as always, Happy Shrooming!


:vaped:



You mean from grain jar to spawn bag, right? :confused2:

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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: ProfessorPinHead]
    #14210195 - 03/30/11 03:41 PM (12 years, 10 months ago)

Pretty sure he was talking about bag to qrts, Professor

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Invisiblehamloaf
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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: ProfessorPinHead]
    #14210200 - 03/30/11 03:42 PM (12 years, 10 months ago)

Quote:

ProfessorPinHead said:
Quote:

hamloaf said:
Any knowledge, advice, useful tips before I begin?  Thanks, have a great day and as always, Happy Shrooming!


:vaped:



You mean from grain jar to spawn bag, right? :confused2:



Nosir.  I'm taking a U-turn on this one and going 5 pound bag of fully colonized WBS into sterile quart jar mediums, then back out to spawn bags once the sterile grain WBS mediums become colonized.  You follow?


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14210221 - 03/30/11 03:47 PM (12 years, 10 months ago)

Yea, I just don't know why?

I usually swing the other way kno what I mean :studmuffin: <- studmuffin is gross!!!! :grrr:

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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: ProfessorPinHead]
    #14210235 - 03/30/11 03:48 PM (12 years, 10 months ago)

I did the same thing as I ran out of room. So it was a way for me to keep it going.

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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: mabachko]
    #14210326 - 03/30/11 04:04 PM (12 years, 10 months ago)

oh, I see. I just always make sure to keep the master spawn  pumpin' :minigun:

I have been in that situation before though, it fuckin' sux!

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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: ProfessorPinHead]
    #14210479 - 03/30/11 04:31 PM (12 years, 10 months ago)

Yea it does, like being all dressed up & nowhere to go.

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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: mabachko]
    #14210528 - 03/30/11 04:38 PM (12 years, 10 months ago)

yea, I stay fresh too def now a daze......

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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: ProfessorPinHead]
    #14210567 - 03/30/11 04:43 PM (12 years, 10 months ago)

soooooooo, we arent going to grow that? i guess we are just going back and forth? keeping it in a mycelium state?  id spawn that to poo befor i cought a case of the clapp and all my hard work was for shits and giggles.


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: jacobensis]
    #14210585 - 03/30/11 04:47 PM (12 years, 10 months ago)

When ur out of room ur out of room, where would you put the bag. It is snowing at this very minute so outside is out.

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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: ProfessorPinHead]
    #14213657 - 03/31/11 07:49 AM (12 years, 10 months ago)

Quote:

why?





For several reasons, really.  The main one being is that culture is needed to be expanded right away in order to stay on the harvest schedule desired and have a bag full of colonized WBS with a known clone culture to work with.  Figured instead of doing bag to bag transfers, prepare about 50 sterile quart jars of WBS knocking up each jar with a few tablespoons or more of fully colonized grains.  That would cut the time it would take to transfer enough mycelium from bag to bag to get the desired amount of bags/tubs needed at once.


Don't know how many quart jars 5 pounds of fully colonized grain spawn will spread out into evenly but I am willing and will soon find out.  Think of it like this, instead of starting your jars to be transfered into bags from agar or LC, use fully colonized grains from a large bag if you have it. 


For me, each quart jar of fully colonized grains represents one monotub after the quart jars have first been expanded into 5-6 pound spawn bags.  I am in complete control of my grows from start to finish so by no means am I out of room for any current project chosen to embark on.  This is mainly just an improvised way of keeping the master spawn running while new cultures are being processed.  If this works out, it wouldn't be anything to me to keep large colonized grain bags for use as mother cultures.


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14213887 - 03/31/11 09:11 AM (12 years, 10 months ago)

I use these from walmart, they are coffee scoops, they are ~ 3 tablespoons 


This way you only need one scoop per jar, the less scoops the better.

I have never had any contamination problems though, I just sterilize the scoop along with the grains in my autoclave. then I keep it in a cup of 91% iso and dip it in between each jar


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: ProfessorPinHead]
    #14214265 - 03/31/11 11:03 AM (12 years, 10 months ago)

Thanks for the tip and yeah, I considered "digging".  I have a myco_2 tablespoon metal spoon that I'v used to scoop spawn out of jars with that were too full or over colonized so tight that conventional methods of breaking apart spawn were not enough.  When the bag is to be opened for transfer, I'll probably just cut the bag diagonally to create a sort of funnel out of the bag, dumping a small amount of colonized grains into each freshly prepared jar.  I want to stay away from "digging" spawn with a spoon.


Instead of PC'ing then storing the spoon inside of a jar of 91% iso alcohol then dipping the spoon in 91% iso alcohol between each use to keep it sterile, I just flame sterilize the spoon with my fatboy butane torch between each use.  Iso alcohol is damaging to mushroom mycelium.  Flame sterilizing the spoon between uses saves the time of having to PC the spoon and the possible cellular damage iso alcohol causes to mycelium, especially at 91% iso.


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14214450 - 03/31/11 11:52 AM (12 years, 10 months ago)

IME Fully colonized grains do not separate easily or "pour out" like dry or even cooked grain will. I use a needle or blade tip to kind of break off colonized grain without damaging the surrounding myc any more than I have to. Probably a little OCD since they are fairly rugged once they are fully colonized.
In any case, have fun.

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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: skyjohnny]
    #14214558 - 03/31/11 12:14 PM (12 years, 10 months ago)

Quote:

IME Fully colonized grains do not separate easily or "pour out" like dry or even cooked grain will.



My humble experience is telling me that you'v never worked with fully colonized grain spawn inside of spawn bags before. 


One of the few beautiful things about creating grain spawn inside of bags over quart jars is that when it comes time to shake, the mycelium can be gently kneaded apart with your finger tips until the colonized grain spawn'll (to use your words) "Pour out like dry or evenly cooked grain will".  Even with fully colonized containers, you are in control of the grain spawn being the consistency fore-mentioned before you even open the bag to retrieve the fully colonized grains.   


I appreciate the feedback, skyjohnny.  When it can be avoided, I like to cause the minimal amount of damage to the mushroom mycleium as humanly possible in all of my procedures and techniques.


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