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OfflineHumility
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Using spawn bags/filter patch bags with a glovebox
    #14210254 - 03/30/11 03:51 PM (12 years, 9 months ago)

Is this feasible?  In RR's documentary he traps a plenum of air in the bag using the flowhood.

would I be able to safely trap a plenum of air in a spawn bag if it's inside of a glovebox?  Wouldn't that be introducing contaminants into the project from day 1?


I looked around a bit using the search engine and didn't find anything specifically addressing this.


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InvisibleDoc_T
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Re: Using spawn bags/filter patch bags with a glovebox [Re: Humility]
    #14210297 - 03/30/11 03:59 PM (12 years, 9 months ago)

You won't be able to draw up that still air without disturbing it and bringing in fresh dirty air.


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OfflineHumility
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Re: Using spawn bags/filter patch bags with a glovebox [Re: Doc_T]
    #14210495 - 03/30/11 04:32 PM (12 years, 9 months ago)

So basically it's impossible to use spawn bags without a flowhood?  I'm glad I took the time to ask this first.  At least I can spend $65 on half gallon/gallon jars instead of not being able to use it at all after buying a bunch of bags.


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Offlinejester1012
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Re: Using spawn bags/filter patch bags with a glovebox [Re: Humility]
    #14210718 - 03/30/11 05:11 PM (12 years, 9 months ago)

Usually the way I do bags is fill em up with my spawn and tightly close the bag, PC them for about 3-4 hours depending on the bag size and nocc them up. My bags have filter patches on them of course so that kind of eliminates the whole letting air in the bag.


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OfflineTerry M
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Re: Using spawn bags/filter patch bags with a glovebox [Re: Humility]
    #14210727 - 03/30/11 05:14 PM (12 years, 9 months ago)

There IS a relatively cheap solution, though. I use a $60 HEPA air purifier for this:

http://www.amazon.com/Hunter-QuietFlo-Purifier-Speeds-30090/dp/B0000X45EY

The air outlet is on the top, and fairly small, so I just turn it on its side. I don't have a flow hood yet, and I've been inflating bags with this very successfully.

BTW, I've taken the plunge today, and am buying stuff for a flow hood. I found a 12"x48" HEPA filter, so I'm gonna have a four foot wide flow hood on my kitchen counter!


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OfflinegitranduhShutr
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Re: Using spawn bags/filter patch bags with a glovebox [Re: Terry M]
    #14211153 - 03/30/11 06:29 PM (12 years, 9 months ago)

you can most definetly use bags with out a flow hood, you must have a glove box though


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Invisibleslapphappypill
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Re: Using spawn bags/filter patch bags with a glovebox [Re: gitranduhShutr]
    #14211362 - 03/30/11 07:04 PM (12 years, 9 months ago)

I seal my bag before PC'ing, then use an injection port of silicon to push LC into my bags. I've been able to do that successfully in open air even. Gotta love oust


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I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
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OfflineRoyalPalm
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Re: Using spawn bags/filter patch bags with a glovebox [Re: slapphappypill]
    #14211612 - 03/30/11 07:48 PM (12 years, 9 months ago)

Quote:

slapphappypill said:
I seal my bag before PC'ing, then use an injection port of silicon to push LC into my bags. I've been able to do that successfully in open air even. Gotta love oust




amen, my current 2.5qt-3.5qt bags are at about 25% and no sign of contamination yet :thumbup:


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OfflineHumility
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Re: Using spawn bags/filter patch bags with a glovebox [Re: RoyalPalm]
    #14212235 - 03/30/11 09:49 PM (12 years, 9 months ago)

Well I have one syringe that's 10ml and another three.

I was going to inoculate 30 jars and do a g2g on some bags and then do another run where I expand the myc from 10 to around 800 and use that to inoculate some jars.

Seems like the G2G run will have to be done using 1/2 gallon jars but the LC run should still work with some modifications.  My guess is "injection port" means get some RTV silicone and make a nub somewhere on the bag that's solid and sturdy then (24 hrs later) inject through it. 

That does sound like it could work but then I run the risk of the LC being bad and contaminating a bunch of bags.  Probably have to only do 3-5 bags after I expand the LC and see if any contaminate and then go from there.

Thanks for the help guys.  Gonna try to shy away from spawn bags until I get a flow hood.  Now i understand why jars are better for a glovebox user.


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OfflineRogerRabbitM
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Re: Using spawn bags/filter patch bags with a glovebox [Re: Humility] * 1
    #14212441 - 03/30/11 10:36 PM (12 years, 9 months ago)

Grain to grain transfers are really hard with spawn bags in a glovebox.  You just don't have enough room in there to work.  If you can pull it off, then after sealing you can pull on the sides of the bag to slowly inflate it through the filter patch.
RR


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OfflineHumility
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Re: Using spawn bags/filter patch bags with a glovebox [Re: RogerRabbit]
    #14215018 - 03/31/11 02:04 PM (12 years, 9 months ago)

I know the questions we post are semi-retarded RR, always seems like it after I hear your simple answers but I'm just not sure you know?

Thanks so much man; of *course* that makes sense.


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InvisibleWise Toad
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Re: Using spawn bags/filter patch bags with a glovebox [Re: Humility]
    #14216750 - 03/31/11 07:27 PM (12 years, 9 months ago)

Interesting, Im about to jump into this sort of work for shiitakes, oysters, and lions mane. Would it be plausible to take 2 boxes and make a GB that looks like a double tub? Have one box on the bottom and one clamped on top with no lid in-between. Add the bags and jars before clamping the boxes together and go to work.


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OfflineHumility
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Re: Using spawn bags/filter patch bags with a glovebox [Re: Wise Toad]
    #14219052 - 04/01/11 04:51 AM (12 years, 9 months ago)

I'm assuming you're going to cut the bottom off of one of the tubs?

Yeah that would work but you need to ensure that all entrances are blocked in order to as best as possible create a "still air environment".

I have been able to find tubs that are more squarish shaped than rectangular.  They are very deep and would easily accomodate a fully opened spawn bag (they are what, 18 inches or so long?  These tubs are at least 18-24 inches deep, possibly 36")

The only thing about these tubs is that the examples I have are opaque.  One is red the other brown.

I'm sure you could find a clear one that's deep and thus allows one to stand up a spawn bag.


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Offlineshroom-jitsu
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Re: Using spawn bags/filter patch bags with a glovebox [Re: Humility]
    #14219532 - 04/01/11 08:28 AM (12 years, 9 months ago)

For what it's worth, I just Lysol my immediate area, open the bags and place the mouth of one inside the other.  Invert, karate-chop and shake until everything is in the desired bag.  Abuse it sufficiently to mix.  The end.  Haven't had contam yet in a bag.  I use my bathroom, as it's a small area easily sprayed.  I also run a hepa for a while before working.

Working with bags in my glovebox significantly raises my blood pressure.


Edited by shroom-jitsu (04/01/11 08:32 AM)


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OfflineRoyalPalm
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Re: Using spawn bags/filter patch bags with a glovebox [Re: shroom-jitsu]
    #14220620 - 04/01/11 12:37 PM (12 years, 9 months ago)

i dont understand all the fuss about filling the bags with air anyway
after pcing a sealed spawn bag they inflate during their cooling proses
:shrug:


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InvisibleFractalXplora
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Re: Using spawn bags/filter patch bags with a glovebox [Re: RoyalPalm]
    #14220707 - 04/01/11 12:49 PM (12 years, 9 months ago)

Quote:

Is this feasible?  In RR's documentary he traps a plenum of air in the bag using the flowhood.

would I be able to safely trap a plenum of air in a spawn bag if it's inside of a glovebox?  Wouldn't that be introducing contaminants into the project from day 1?




i do this al the time and have no problems in my glovebox, hardily ever had a shiitake bag go bad, i just use a nice bleach cleaned, well sprayed alcohol glovebox, far form ideal, but works until I can get a flowhood sorted out.:thumbup:


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OfflineHumility
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Re: Using spawn bags/filter patch bags with a glovebox [Re: FractalXplora]
    #14222218 - 04/01/11 05:18 PM (12 years, 9 months ago)

This thread has really set my mind at ease.  Thanks a lot for all of the replies and if anything is relevant or current please feel free to update.

I try to not create new threads but I couldn't find anything specifically addressing this anywhere on the forums.

Again, thanks a lot guys.  I'm just going to buy some bags and see if it works or not.  The work I'd be shaving off by doing 4 bags instead of 8 jars (not to mention there is greater volume in 4 bags than 8 jars) at a time makes things a lot easier.


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OfflineRoyalPalm
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Re: Using spawn bags/filter patch bags with a glovebox [Re: Humility]
    #14222359 - 04/01/11 05:48 PM (12 years, 9 months ago)

:thumbup: awesome
the only real con i have noticed to using bags is colonization time ,that could easily be due to my water
content being off as ive only preped bags twice so far
depending on your situation like your said its convenient in other departments 

Heres two MAJOR tip that i wish i would have known
bags will try to inflate in the pc so  use lots of aluminum foil to protect the bags from the walls of the pc
and to create gaps between the bags for steam to flow. also dont forget to a heavy to put a plate ontop so the bags can not clog the steam vent ect.


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:heart: spread the love :heart:
:bow2: www.khanacademy.org :bow2: www.daveconservatoire.org :bow2:
Quality Dream Catchers(any size you want & many very creative shapes) Flower head bands
Hemp, Leather, and Synthetic fiber slings (stone throwers) for sale/trade.PM for details
Learn more about the ancient art at www.slinging.org
Ps. RC vendor/ review/ forum invite porfavor ?


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