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Offlinehamloaf
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Grain to Grain Transfer From Spawn Bag to Quart Jar.
    #14209841 - 03/30/11 02:23 PM (12 years, 9 months ago)

Any knowledge, advice, useful tips before I begin?  Thanks, have a great day and as always, Happy Shrooming!


:vaped:


Update: 4-16-11


Transferring fully colonized grains from bag to jar was a piece of cake and went a lot soother than anticipated. 


Using the 5 quart bag of fully colonized grains (WBS), was able to inoculate all 27 horse manure+ tea jars,


 





with this much grains left over.








With the 9 quart spawn bag, 45 jars were able to be inoculated,





With this much grains left over.








Let's see what culminates.  :popcorn:


Update, 4-21-11


So far, so good. 





Bout to shake in a few.


Update: 4/23,


Jars are recovering nicely after just 1-2 days from the mycelium being shook apart.  Not all of the jars were shook at the same time due to time constraints but were shook within 6 hours from the time the first round of jars were shook. 








While the jars were being sorted through, 2 of 72 jars, thus far have become contaminated with 2 separate kinds of bacteria. 


One was cob-web mold.





The other of coarse, trichoderma. 








These last three jars were found to be well over 95% colonization so the decision was made to not shake these jars. 





Update,  4-28-11



Almost there.......... (at 100% colonization). 





Rye suppliers harvested.  Got 400 pounds of it (rye #1), for 70 bux. 





Like how clean, freshly harvested rye can be from time to time.  This is the rye just after filling the pot with water and then stirring for the first time, no rinse yet. 





Hell yeah.  This rye only took one rinse with hot water and stirred before it was clean enough to add gypsum, beginning the soak duration.  That's going to considerably save some time spent rinsing the rye. 





Time to get to work prepping the grains to be loaded into bags and then sterilized.  More to come.  :popcorn:



update 5-3-11


Been there for a few days now (at 100% colonization).





Started the bags last weeks but due to life happening, had to halt production.


Between 2 25x electric AA steroclaves and an AA, model 941 PC, 14, 6 quart spawn bags filled with properly prepared rye were able to be sterilized at once.


The sterile bags were removed from the PC, wiped with an alcohol soaked paper towel and placed into a few large storage totes that were also wiped down with an alcohol soaked paper towel to safely store the sterile bags while production continues.


update 5-3-11


Been there for a few days now (at 100% colonization).





Started the bags last weeks but due to life happening, had to halt production.


Between 2 25x electric AA steroclaves and an AA, model 941 PC, 14, 6 quart spawn bags filled with properly prepared rye were able to be sterilized at once.


The sterile bags were removed from the PC, wiped with an alcohol soaked paper towel and placed into a few large storage totes that were also wiped down with an alcohol soaked paper towel to safely store the sterile bags while production continues.





My region's climate is going to be experiencing hot dry weather for weeks to come.  It's about time to spread the horse manure over a tarp and begin shredding and further dehydrating it out in that hot sun.


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Edited by liquidmyce (05/03/11 10:35 PM)


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Offlinemabachko
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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14209876 - 03/30/11 02:30 PM (12 years, 9 months ago)

I just did that the other day and using a GB is a PITA but it can be done
I only used 5 qrt jars for the 1 pound of rye bag that was colonized. I also had 7 1/2 pints that I wanted to try a G2BRF. That was probably why it was a PITA. Not much room to work in.
But all went well.
I wipe the bag and all other items down with alcohol prior to putting them into the GB, then sprayed Lysol & closed the lid. Waited about 10 minutes & started the deed.
So far no contams.
Good Luck


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: mabachko]
    #14209972 - 03/30/11 02:45 PM (12 years, 9 months ago)

Quote:

mabachko said:
I just did that the other day and using a GB is a PITA but it can be done
I only used 5 qrt jars for the 1 pound of rye bag that was colonized. I also had 7 1/2 pints that I wanted to try a G2BRF. That was probably why it was a PITA. Not much room to work in.
But all went well.
I wipe the bag and all other items down with alcohol prior to putting them into the GB, then sprayed Lysol & closed the lid. Waited about 10 minutes & started the deed.
So far no contams.
Good Luck



Sounds good, my man.  I hope all works out well for you.  :mushroom2:


Thanks for the advise.  Will be working with 5 pound bag of fully colonized WBS and I have a flowhood.  That's a good idea, wiping all cultivation tools and items with an alcohol soaked paper towel before I move them in front of the flowhood or inside of a still air box and is part of my normal sterile procedure.


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14210033 - 03/30/11 03:02 PM (12 years, 9 months ago)

Never done G2BRF. Keep us posted. May the shrooms smile upon your endeavor.


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: skyjohnny]
    #14210120 - 03/30/11 03:27 PM (12 years, 9 months ago)

Posted a pic last night.



But it has doubled in growth since then.
Was told it was a nono so I started it to see what happens.
Will update as time goes on.


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14210163 - 03/30/11 03:36 PM (12 years, 9 months ago)

Quote:

hamloaf said:
Any knowledge, advice, useful tips before I begin?  Thanks, have a great day and as always, Happy Shrooming!


:vaped:



You mean from grain jar to spawn bag, right? :confused2:


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: ProfessorPinHead]
    #14210195 - 03/30/11 03:41 PM (12 years, 9 months ago)

Pretty sure he was talking about bag to qrts, Professor


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: ProfessorPinHead]
    #14210200 - 03/30/11 03:42 PM (12 years, 9 months ago)

Quote:

ProfessorPinHead said:
Quote:

hamloaf said:
Any knowledge, advice, useful tips before I begin?  Thanks, have a great day and as always, Happy Shrooming!


:vaped:



You mean from grain jar to spawn bag, right? :confused2:



Nosir.  I'm taking a U-turn on this one and going 5 pound bag of fully colonized WBS into sterile quart jar mediums, then back out to spawn bags once the sterile grain WBS mediums become colonized.  You follow?


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14210221 - 03/30/11 03:47 PM (12 years, 9 months ago)

Yea, I just don't know why?

I usually swing the other way kno what I mean :studmuffin: <- studmuffin is gross!!!! :grrr:


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: ProfessorPinHead]
    #14210235 - 03/30/11 03:48 PM (12 years, 9 months ago)

I did the same thing as I ran out of room. So it was a way for me to keep it going.


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: mabachko]
    #14210326 - 03/30/11 04:04 PM (12 years, 9 months ago)

oh, I see. I just always make sure to keep the master spawn  pumpin' :minigun:

I have been in that situation before though, it fuckin' sux!


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: ProfessorPinHead]
    #14210479 - 03/30/11 04:31 PM (12 years, 9 months ago)

Yea it does, like being all dressed up & nowhere to go.


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: mabachko]
    #14210528 - 03/30/11 04:38 PM (12 years, 9 months ago)

yea, I stay fresh too def now a daze......


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: ProfessorPinHead]
    #14210567 - 03/30/11 04:43 PM (12 years, 9 months ago)

soooooooo, we arent going to grow that? i guess we are just going back and forth? keeping it in a mycelium state?  id spawn that to poo befor i cought a case of the clapp and all my hard work was for shits and giggles.


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: jacobensis]
    #14210585 - 03/30/11 04:47 PM (12 years, 9 months ago)

When ur out of room ur out of room, where would you put the bag. It is snowing at this very minute so outside is out.


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: ProfessorPinHead]
    #14213657 - 03/31/11 07:49 AM (12 years, 9 months ago)

Quote:

why?





For several reasons, really.  The main one being is that culture is needed to be expanded right away in order to stay on the harvest schedule desired and have a bag full of colonized WBS with a known clone culture to work with.  Figured instead of doing bag to bag transfers, prepare about 50 sterile quart jars of WBS knocking up each jar with a few tablespoons or more of fully colonized grains.  That would cut the time it would take to transfer enough mycelium from bag to bag to get the desired amount of bags/tubs needed at once.


Don't know how many quart jars 5 pounds of fully colonized grain spawn will spread out into evenly but I am willing and will soon find out.  Think of it like this, instead of starting your jars to be transfered into bags from agar or LC, use fully colonized grains from a large bag if you have it. 


For me, each quart jar of fully colonized grains represents one monotub after the quart jars have first been expanded into 5-6 pound spawn bags.  I am in complete control of my grows from start to finish so by no means am I out of room for any current project chosen to embark on.  This is mainly just an improvised way of keeping the master spawn running while new cultures are being processed.  If this works out, it wouldn't be anything to me to keep large colonized grain bags for use as mother cultures.


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14213887 - 03/31/11 09:11 AM (12 years, 9 months ago)

I use these from walmart, they are coffee scoops, they are ~ 3 tablespoons 


This way you only need one scoop per jar, the less scoops the better.

I have never had any contamination problems though, I just sterilize the scoop along with the grains in my autoclave. then I keep it in a cup of 91% iso and dip it in between each jar



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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: ProfessorPinHead]
    #14214265 - 03/31/11 11:03 AM (12 years, 9 months ago)

Thanks for the tip and yeah, I considered "digging".  I have a myco_2 tablespoon metal spoon that I'v used to scoop spawn out of jars with that were too full or over colonized so tight that conventional methods of breaking apart spawn were not enough.  When the bag is to be opened for transfer, I'll probably just cut the bag diagonally to create a sort of funnel out of the bag, dumping a small amount of colonized grains into each freshly prepared jar.  I want to stay away from "digging" spawn with a spoon.


Instead of PC'ing then storing the spoon inside of a jar of 91% iso alcohol then dipping the spoon in 91% iso alcohol between each use to keep it sterile, I just flame sterilize the spoon with my fatboy butane torch between each use.  Iso alcohol is damaging to mushroom mycelium.  Flame sterilizing the spoon between uses saves the time of having to PC the spoon and the possible cellular damage iso alcohol causes to mycelium, especially at 91% iso.


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14214450 - 03/31/11 11:52 AM (12 years, 9 months ago)

IME Fully colonized grains do not separate easily or "pour out" like dry or even cooked grain will. I use a needle or blade tip to kind of break off colonized grain without damaging the surrounding myc any more than I have to. Probably a little OCD since they are fairly rugged once they are fully colonized.
In any case, have fun.


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: skyjohnny]
    #14214558 - 03/31/11 12:14 PM (12 years, 9 months ago)

Quote:

IME Fully colonized grains do not separate easily or "pour out" like dry or even cooked grain will.



My humble experience is telling me that you'v never worked with fully colonized grain spawn inside of spawn bags before. 


One of the few beautiful things about creating grain spawn inside of bags over quart jars is that when it comes time to shake, the mycelium can be gently kneaded apart with your finger tips until the colonized grain spawn'll (to use your words) "Pour out like dry or evenly cooked grain will".  Even with fully colonized containers, you are in control of the grain spawn being the consistency fore-mentioned before you even open the bag to retrieve the fully colonized grains.   


I appreciate the feedback, skyjohnny.  When it can be avoided, I like to cause the minimal amount of damage to the mushroom mycleium as humanly possible in all of my procedures and techniques.


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14214593 - 03/31/11 12:24 PM (12 years, 9 months ago)

agree, I've recently started using bags myself, and not sure if i'll go back to jars..for the simple reason I always hurt my hand breaking up the mycelium lol


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: biologys]
    #14214719 - 03/31/11 12:56 PM (12 years, 9 months ago)

Quote:

biologys said:
agree, I've recently started using bags myself, and not sure if i'll go back to jars..for the simple reason I always hurt my hand breaking up the mycelium lol




To me, it's not about the use of one container over another in a question of which is best.  I use quart jars and spawn bags.  1 fully colonized quart jar of grain spawn into 1 freshly sterilized 5-6 pound grain bag medium.


When transferring colonized grain spawn into large grain bags from quart jars, the mycelium only has to be broken up enough to be allowed to escape the mother jar.  Once the grain spawn is inside of the bag, you can gently knead apart the mycelium clumps before or after you seal the bag.


I'v recently begun to think that wide-mouthed pint-sized jars would be ideal for this practice.  Just pop off the lid, dump the entire colonized grain cake into the bag, and break it apart inside of the bag.  That would eliminate having to bang jars on allotted surfaces and all the possible side effects involved with "banging jars" including your grievance of aching hands caused by "banging jars".  Less is more.


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14214892 - 03/31/11 01:35 PM (12 years, 9 months ago)

Quote:


For me, each quart jar of fully colonized grains represents one monotub after the quart jars have first been expanded into 5-6 pound spawn bags.




I just tried this as well hamloaf, good thinking, the one quart to spawn bags saves a lot of jar and tyvek hassle, cant believe I not tried this before. :facepalm: perfect amount of spawn for a decent size tub:thumbup: ;less space, quicker colonization.....


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: FractalXplora]
    #14228337 - 04/02/11 09:11 PM (12 years, 9 months ago)

Thanks man but I think people have been inoculating large grain substrates with 1 quart of fully colonized grains for a while now.


Yes.  Using 1 quart of fully colonized grains to inoculate 5-6 quart spawn bags'll have all that grain colonized in 7-10 days, granted you shake/knead the mycelium apart at 20-70% colonization and your mother jar's mycelium is free of bacterial spores.


I like to be sure to use 1 jar per one bag instead of using 1 jar to inoculate several bags.  That way, any contamination caused from the mother jar'll be isolated into only one bag instead of several bags at a time, taking some guess work out of knowing where the contamination came from.


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14228349 - 04/02/11 09:14 PM (12 years, 9 months ago)

:studmuffin: I figured you guys needed a little studmuffin' in your night.


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: ProfessorPinHead]
    #14228409 - 04/02/11 09:23 PM (12 years, 9 months ago)

Sheeit Professor Pinhead, I'm stud muffin enough for every night.  :super:


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14243031 - 04/05/11 05:11 PM (12 years, 9 months ago)

Ok, so I was able to squeeze 27 quart jars that were 3/4th the way full (gotta' leave room for the entry of colonized grains) out of 1, 20 pound bag of premium birdseed mix prepared to be sterilized.


Soaked this batch of birdseed in about a 35% concentrate horse manure tea water along with 3% by volume of gypsum for 10 hours (going for that slow hydration) and boiled the WBS for ten minutes, allowing the WBS to reach the proper surface hydration level for quart jar inoculations of fully colonized grains before loading the WBS into jars.


18 0f the 27 jars are sterilized and cooling to room temperatures.  More to come.  :popcorn:


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14243116 - 04/05/11 05:29 PM (12 years, 9 months ago)

:thumbup:


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: ProfessorPinHead]
    #14243155 - 04/05/11 05:36 PM (12 years, 9 months ago)

:thumbup:  :thumbup:


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: penhed]
    #14247989 - 04/06/11 03:13 PM (12 years, 9 months ago)

Good Luck, butI would have done a large batch.Ha!


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: mabachko]
    #14250685 - 04/06/11 11:22 PM (12 years, 9 months ago)

!


Edited by mattman (01/07/12 02:08 AM)


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: mattman]
    #14251662 - 04/07/11 05:50 AM (12 years, 9 months ago)

Quote:

mattman said:
I use a rolled up softcover book (like a phone book) to break up jars.

I was getting achy hands from beating jars!



you should try a bike tire. It is sick!


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: ProfessorPinHead]
    #14255963 - 04/07/11 10:01 PM (12 years, 9 months ago)

.


Edited by mattman (01/07/12 02:07 AM)


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: mattman]
    #14255965 - 04/07/11 10:02 PM (12 years, 9 months ago)

Quote:

mattman said:
I use a rolled up softcover book (like a phone book) to break up jars.

I was getting achy hands from beating jars!





I use a phone book that I have wrapped with like 3 layers of gorilla tape..  Works great lmao..


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: Damion5050]
    #14261766 - 04/09/11 03:12 AM (12 years, 9 months ago)

haha, A rolled up and bound in duct tape phone book is what I use to break my spawn in jars apart with. 





Said fuck it and decided to prepare 4 times the amount of jar mediums to be inoculated with colonized grains from bags.  The inoculations are scheduled to take place Monday.  For shits and giggles, as well as, for all you myco_voyeurs out there, here goes a shot of the fully colonized bags that are planned to be transfered into quart jars.





The bag on the far left is the bag that'll be transfered first.  It contains 5 quarts of fully colonized WBS.  The 2 bags on the left each have a whopping 9 quarts of fully colonized WBS and clean smelling mushroom mycelium in each one.


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14261891 - 04/09/11 05:24 AM (12 years, 9 months ago)

Yea, it looked like the levels were off a little bit, I wasn't sure if you were just  :getstoned:  when you prepped them.


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: ProfessorPinHead]
    #14261900 - 04/09/11 05:31 AM (12 years, 9 months ago)

Quote:

ProfessorPinHead said:
Yea, it looked like the levels were off a little bit, I wasn't sure if you were just  :getstoned:  when you prepped them.



I think you are  :getstoned:  while you made this post.  What do you mean, "it looked like my levels where off a little bit".  Says who?  Who dictates at what level grains are to be filled into bags for making grain spawn?


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14261915 - 04/09/11 05:41 AM (12 years, 9 months ago)


"The 2 bags on the left each have a whopping 9 quarts of fully colonized WBS and clean smelling mushroom mycelium in each one."





The one 5 quart bag looked like it was smaller than the other two, that all I meant by it. :smile:

I was just joking around.


I just smoked though, so you were almost right about that one,. .... :getstoned:


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14261923 - 04/09/11 05:51 AM (12 years, 9 months ago)

Quote:

hamloaf said:

Nosir.  I'm taking a U-turn on this one and going 5 pound bag of fully colonized WBS into sterile quart jar mediums, then back out to spawn bags once the sterile grain WBS mediums become colonized.  You follow?




Why? :shrug:

Why not grain to grain transfer from bag to bag?  Going to jars and then back again just adds another step with no benefit.
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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: RogerRabbit]
    #14261943 - 04/09/11 06:09 AM (12 years, 9 months ago)

Well, to buy some time basically.  Don't currently have the grains on hand to fill the amount of spawn bags needed to do the amount of bag transfers needed to stay on top of the usual growth regimen.  Let alone the spawn bags to facilitate.  What I do have are jars, some birdseed, and the will to succeed.


I'm aware of the extra step added, as well as, there being no real benefit from jar to bag, back to jar, then out to bag again.  Just wound up in a situation that needed to improvised upon and adapted too in order for the situation to be over came.  It's been a hectic last month.  Waiting for my grain mill to harvest their rye (which should be any day now).  Also, waiting to get paid before the ability to pick up more spawn bags will be allotted.


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14298970 - 04/16/11 12:21 AM (12 years, 9 months ago)

Transferring fully colonized grains from bag to jar was a piece of cake and went a lot soother than anticipated. 


Using the 5 quart bag of fully colonized grains (WBS), was able to inoculate all 27 horse manure+ tea jars,


 



with this much grains left over.








With the 9 quart spawn bag, 45 jars were able to be inoculated,






With this much grains left over.


 



Let's see what culminates.  :popcorn:


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14304712 - 04/17/11 05:08 AM (12 years, 9 months ago)

You should throw the remaining grain outside for the birds to eat. :minigun:

Thats what I do! :thumbup:


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: ProfessorPinHead]
    #14305147 - 04/17/11 08:57 AM (12 years, 9 months ago)

whats the weapon of choice, young bird hunter?  Lol


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: drosmoka]
    #14327007 - 04/21/11 10:00 AM (12 years, 9 months ago)

So far, so good. 





Bout to shake in a few.


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14327056 - 04/21/11 10:12 AM (12 years, 9 months ago)

:thumbup:


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: EvilMushroom666]
    #14327061 - 04/21/11 10:13 AM (12 years, 9 months ago)

holy shit :mushroom2:


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: tripdawg420]
    #14327173 - 04/21/11 10:46 AM (12 years, 9 months ago)

nice post
where did you purchase the bags from?
did you purhcase your lids?
how do you steralize your bags?


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: realcarlos]
    #14327268 - 04/21/11 11:16 AM (12 years, 9 months ago)

:popcorn:


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: realcarlos]
    #14327354 - 04/21/11 11:41 AM (12 years, 9 months ago)

Quote:

realcarlos said:
nice post
where did you purchase the bags from?
did you purhcase your lids?
how do you steralize your bags?






Glad you'r enjoying this.


I source my bags from 2 places, Hoosier Mushroom Company and S.E. Mushrooms.  The particular bags featured in this thread today are large gusseted, .5um filter patched spawn bags from S.E. Mushrooms.  You can purchase bags from them directly or find their deals on Ebay. 


I constructed the lids myself.  I used regular PP5 plastic lids designed and used for canning, poked 2 1/16th" holes for GE and covered the holes with synthetic filter disks cut down to the size of quarter for the GE filter, and installed injection ports.  I don't inoculate with syringes anymore so all my new lid designs no longer come with injection ports.  Just 2 holes punched into the center of the lid covered with a piece of synthetic filter disk.


I fill bags with 5-6 quarts of cooked and hydrated grains.  I don't pre-seal bags before sterilization rather use the whole tyvek wrist sleeve stuffed down the folds of the bag then folded accordion style as per RR's methodology.  Bags are sterilized @ 20 psi's for 2 hours with a ceramic plate placed over the bags to keep them depressed during the sterilization cycle, let cool to room temps, and inoculated with 1 quart of fully colonized grains.  The bags are sealed in front of a flowhood, capturing a plenum of sterile air, twist tied at the top of the bags, and closed using plastic zip ties.


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Edited by liquidmyce (04/21/11 11:47 AM)


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14327409 - 04/21/11 11:58 AM (12 years, 9 months ago)

thank you ham for the detailed explanation


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: realcarlos]
    #14328622 - 04/21/11 04:30 PM (12 years, 9 months ago)

NO problem, man.  Hope that helps you.  :hatsoff:


I like to taking the time to explain and talk about one of my most favorite subjects on the planet.  Are you considering making the switch to bags or do you have some experience with making spawn in bags before?


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14328829 - 04/21/11 05:11 PM (12 years, 9 months ago)

i dont have experience this is only my 2nd failed attempt at brf hoping to move onto grain


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: realcarlos]
    #14328985 - 04/21/11 05:42 PM (12 years, 9 months ago)

OK I think I'm following? If 1qt jar means 1 mono, then 72 monos is going to be a serious  pita at harvest time! Wow!  :headbang:  :goodluck:


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: realcarlos]
    #14331889 - 04/22/11 06:19 AM (12 years, 9 months ago)

Quote:

realcarlos said:
i dont have experience this is only my 2nd failed attempt at brf hoping to move onto grain



I feel you man.  Go for the grains.  Once you find a good source for grains, grains are pretty cheap in comparison to purchasing bags of pre-ground, organic BRF.  Plus grains can be ground into flour and substituted for BRF in BRF cakes.  Hand ground grain flour is better than pre-ground organic BRF.   


Not the best idea to jump into bags if you havn't any experience with grains.  Best thing you can do if you wish to start with grains is start small, go around the learning curve of grains a few times, until you gain a feel for and know where to source the materials your working with in your own specific, unique climate/environment and grow situation.  Get you case of quart jars and a bag of WBS or rye and follow RR's grain preparation technique that can be found in his videos that you can see samples of if you look in his sig..


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14332283 - 04/22/11 08:47 AM (12 years, 9 months ago)

thanks ham really appreciate it


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: shroomybgood]
    #14337186 - 04/23/11 05:59 AM (12 years, 9 months ago)

Quote:

shroomybgood said:
OK I think I'm following? If 1qt jar means 1 mono, then 72 monos is going to be a serious  pita at harvest time! Wow!  :headbang:  :goodluck:



Just another day at the office.  Lmao  Harvest time is not what I'm concerned about.  For me, harvest time is the time to enjoy the fruits of your labor.  The step I'm dreading the most (truth be told) is the spawning all that spawn to it's respected bulk substrate.  For some reason, spawning to bulk is my least favorite of all the steps involved in cultivation.  Maybe it's because of the preparation of all that bulk substrate materials involved in that step.  :justdontknow:


Either way, 50/50 horse manure/coir+'ll be used as the base, bulk substrate.  Thinking of adding equal parts wheat straw just to spread the manure and coir that much further, adding another varying source of nutrition for the mushroom mycelium without getting too crazy with the additives, as well as, while in spring months, with the summer months approaching, my region's ambient humidity is rising.  IMHE. straw based substrates perform better in humid climates for the straw doesn't dry out as fast as it would if the straw was being used as a bulk substrate material in dry climates, unless your using wheat straw as a bulk substrate material inside of a sterile environment.


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14337458 - 04/23/11 08:27 AM (12 years, 9 months ago)

Wow, I can't believe that slipped my mind. Agreed, that substrate is definitely going to be a BITCH! Worth every aching hour at harvest time though. You have inspired me!


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: shroomybgood]
    #14340482 - 04/23/11 08:36 PM (12 years, 9 months ago)

Good to know, bro.  If I can inspire at least one soul around here then I know my job's being done.  Thank you for your words of encouragement, shroomeybgood.  :hatsoff:


Update: 4/23,


Jars are recovering nicely after just 1-2 days from the mycelium being shook apart.  Not all of the jars were shook at the same time due to time constraints but were shook within 6 hours from the time the first round of jars were shook.   








While the jars were being sorted through, 2 of 72 jars, thus far have become contaminated with 2 separate kinds of bacteria. 


One was cob-web mold.





The other of coarse, trichoderma. 








These last three jars were found to be well over 95% colonization so the decision was made to not shake these jars. 






*All updates have been edited into the original post.  Thanks again for viewing this thread.


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14366367 - 04/28/11 11:19 AM (12 years, 9 months ago)

Almost there.......... (at 100% colonization). 





Rye suppliers harvested.  Got 400 pounds of it (rye #1), for 70 bux. 





Like how clean, freshly harvested rye can be from time to time.  This is the rye just after filling the pot with water and then stirring for the first time, no rinse yet. 





Hell yeah.  This rye only took one rinse with hot water and stirred before it was clean enough to add gypsum, beginning the soak duration.  That's going to considerably save some time spent rinsing the rye. 





Time to get to work prepping the grains to be loaded into bags and then sterilized.  More to come.  :popcorn:


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14366452 - 04/28/11 11:36 AM (12 years, 9 months ago)

:popcorn:


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First:                                                        Then:


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #14366457 - 04/28/11 11:37 AM (12 years, 9 months ago)

dwamn hamn


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: k00laid]
    #14389717 - 05/02/11 04:35 PM (12 years, 8 months ago)

:dancingbear:


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: ProfessorPinHead]
    #14389812 - 05/02/11 04:51 PM (12 years, 8 months ago)

Good details and pictures.  Watching  :laugh:


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: Alkaloids]
    #14397347 - 05/03/11 10:35 PM (12 years, 8 months ago)

Thanks.  :mushroom2:


Update 5-3-11


Been there for a few days now (at 100% colonization).





Started the bags last weeks but due to life happening, had to halt production.


Between 2 25x electric AA steroclaves and an AA, model 941 PC, 14, 6 quart spawn bags filled with properly prepared rye were able to be sterilized at once.


The sterile bags were removed from the PC, wiped with an alcohol soaked paper towel and placed into a few large storage totes that were also wiped down with an alcohol soaked paper towel to safely store the sterile bags while production continues.





My region's climate is going to be experiencing hot dry weather for weeks to come.  It's about time to spread the horse manure over a tarp and begin shredding and further dehydrating it out in that sun.


More to come.  :popcorn:


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #15537029 - 12/18/11 08:36 PM (12 years, 1 month ago)

One word to describe this research experiment= SENESCENCE!

The jars posted above were T3 from a bag of colonized grains inoculated with T2 fully colonized grain jars from mother jars that were inoculated with a known-to-be-clean LC.  Each fully colonized jar were then, transferred out into a 5 quart spawn bag (T4).

After T4, which ever bag didn't subcombe to bacteria or mold displayed weak looking and, slow growing mycelium.  Any bag that DID make it bulk spawning behaved in the same manner as the mycelium colonizing the grain spawn after the fourth transfer (T4), either colonized slow, was weak looking or, became bacterial or molded.  Out of 72 T3 jars, must have had somewhere in the neighborhood of 8-12 tubs (I can't remember exactly how many) flush at least once, prolifically.


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: mabachko]
    #19712467 - 03/18/14 10:14 AM (9 years, 10 months ago)

How did that jar turn out from the g2g transfer? I'm looking at doing that tonight, any recommendations?


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: blackflag856]
    #19767524 - 03/30/14 06:49 AM (9 years, 9 months ago)

It worked out pretty good, man.  It was pretty easy to transfer the grains into the jars from a bag.

In hindsight though, I wish I would have gone with RR's suggestion of transferring that bag into more bags.  Transferring from bag to bag would have created me more spawn at about 1/8th of the effort.


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Re: Grain to Grain Transfer From Spawn Bag to Quart Jar. [Re: hamloaf]
    #20117951 - 06/12/14 04:37 AM (9 years, 7 months ago)

great log and i wish i could still see the pictures.


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