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swamp ass
Stranger


Registered: 09/21/10
Posts: 76
Last seen: 5 years, 1 month
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Its bacteria right?
#14209074 - 03/30/11 11:38 AM (12 years, 9 months ago) |
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Right?
-------------------- "We're bored. We're all bored now. But has it ever occurred to you, Wally, that the process that creates this boredom that we see in the world now, may very well be a self perpetuating, unconscious form of brainwashing created by a world totalitarian government based on money and that all of this is much more dangerous than one thinks, and it's not just a question of individual survival, Wally, but that somebody who's bored is asleep, and somebody who's asleep will not say no?"
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Boozie
I like beer.



Registered: 02/18/10
Posts: 1,226
Loc: :ↄo⅃
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Re: Its bacteria right? [Re: swamp ass]
#14209826 - 03/30/11 02:20 PM (12 years, 9 months ago) |
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Yessiree.
-------------------- "After silence, that which comes nearest to expressing the inexpressible is music." - Aldous Huxley
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Its bacteria right? [Re: Boozie] 2
#14209858 - 03/30/11 02:27 PM (12 years, 9 months ago) |
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Agreed.
Good news: you can identify bacterial contam now.
-------------------- You make it all possible. Doesn't it feel good?
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solumvita
Q.B.E.


Registered: 02/12/08
Posts: 2,061
Loc: South Africa
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Re: Its bacteria right? [Re: Doc_T]
#14225972 - 04/02/11 01:09 PM (12 years, 9 months ago) |
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Quote:
Doc_T said: Agreed.
Good news: you can identify bacterial contam now.
-------------------- One of these days all the answers will be revealed until then we learn from each other! www.mushrush.co.za
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Mycelio
Stranger


Registered: 06/24/08
Posts: 1,636
Loc: Berlin
Last seen: 3 months, 23 days
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Re: Its bacteria right? [Re: swamp ass]
#14226960 - 04/02/11 04:29 PM (12 years, 9 months ago) |
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Looks like bakers yeast. Does it have an alcoholic smell?
In any case, avoid excess water in your grain and improve your sterile procedures.
Carsten
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swamp ass
Stranger


Registered: 09/21/10
Posts: 76
Last seen: 5 years, 1 month
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Re: Its bacteria right? [Re: Mycelio]
#14229594 - 04/03/11 01:38 AM (12 years, 9 months ago) |
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Quote:
Doc_T said: Agreed.
Good news: you can identify bacterial contam now.
lol
Quote:
Mycelio said: Looks like bakers yeast. Does it have an alcoholic smell?
In any case, avoid excess water in your grain and improve your sterile procedures.
Carsten
Kind of yeah. The jars were knocked up with 90% colonised stalled pf jar, thats most probably the reason it got contaminated. I wasnt hoping anything from them anyway, it was just a test.
-------------------- "We're bored. We're all bored now. But has it ever occurred to you, Wally, that the process that creates this boredom that we see in the world now, may very well be a self perpetuating, unconscious form of brainwashing created by a world totalitarian government based on money and that all of this is much more dangerous than one thinks, and it's not just a question of individual survival, Wally, but that somebody who's bored is asleep, and somebody who's asleep will not say no?"
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SomeGuy
I feel better now :)


Registered: 04/18/10
Posts: 7,496
Loc:
Last seen: 9 years, 8 months
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Re: Its bacteria right? [Re: swamp ass]
#14230741 - 04/03/11 11:22 AM (12 years, 9 months ago) |
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You'll get it. Sometimes it takes a couple of tries
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
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Re: Its bacteria right? [Re: SomeGuy]
#14231639 - 04/03/11 03:35 PM (12 years, 9 months ago) |
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Quote:
Its bacteria right?
Right?
Correct, bacteria. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Deej3987
Deej3987

Registered: 12/08/09
Posts: 128
Last seen: 1 year, 5 months
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I have the same problem with my jars every once in a while.. after I pressure cook the jars and they are sterile what are some ways the bacteria can get in there? Ive been doing G2G transfers with rye grains in a glove box.. I wipe down every jar with the highest % alcohol I can buy and spray the air with lyscol before I put them into the glovebox. I even have alcohol spray inside the glovebox to be even more sterile... It smells like alcohol so bad in there when I open it up I don't know how any bacteria could get in there?
What is the main cause of this bacteria? after hydrating beens making sure the grains are dry? thats the only thing I can think of since the grains get so wet when the bacteria is ther.. but even then I don't have this bacteria problem until after the G2G transfer...
-------------------- My Trade List Printing All info I post about myself, what I am doing, and what I have done is fake; and therefore, holds no truth and can not be used to prove any previous action of mine.
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SomeGuy
I feel better now :)


Registered: 04/18/10
Posts: 7,496
Loc:
Last seen: 9 years, 8 months
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Re: Its bacteria right? [Re: Deej3987]
#14231975 - 04/03/11 04:39 PM (12 years, 9 months ago) |
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bumping the sides of your glovebax causes air to be sucked in. Try to be really gentle and move slowely without bumping the top/sides
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Deej3987
Deej3987

Registered: 12/08/09
Posts: 128
Last seen: 1 year, 5 months
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Re: Its bacteria right? [Re: SomeGuy]
#14244236 - 04/05/11 08:32 PM (12 years, 9 months ago) |
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I'm going to customize my glovebox, make some improvements to it.. also pressure cook my syringes and make sure they are sterile. I'm starting to think the bacteria is there from the beginning and is just a mater of time before it dominates later transfers... also agar is another thing I'm going to attempt again if the problem persists.
-------------------- My Trade List Printing All info I post about myself, what I am doing, and what I have done is fake; and therefore, holds no truth and can not be used to prove any previous action of mine.
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PulseEden
Cyborg



Registered: 01/13/10
Posts: 140
Last seen: 11 years, 11 months
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Re: Its bacteria right? [Re: Deej3987]
#14248918 - 04/06/11 05:58 PM (12 years, 9 months ago) |
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If you have a glass fishtank you don't use make a glove box out of that. Get a piece of plexi glass, cut it to fit the top of the tank and then cut two holes in it for your arms that are no more than a half inch wider than the widest part of your forearms. Lay the plexi ontop of the tank, duct tape it off all around the edges. Eliminates the suction issue from bumping your glovebox.
Keep in mind that opening this up is annoying, so if you're going to have to put things in it that won't fit through the arm holes, make a door in the plexi glass to the side for bigger stuff.
-------------------- I booped your daughter! A boop boop boop!
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liquidlounge

Registered: 12/22/10
Posts: 9,256
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Re: Its bacteria right? [Re: PulseEden]
#14266796 - 04/10/11 12:10 PM (12 years, 9 months ago) |
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How can you see its bacteria in that one? For me it looks good.
-------------------- As far as I assume to know...
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Humility
Working on it



Registered: 10/07/08
Posts: 6,745
Last seen: 6 years, 11 months
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Re: Its bacteria right? [Re: Deej3987]
#14267903 - 04/10/11 04:48 PM (12 years, 9 months ago) |
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Quote:
Deej3987 said: I have the same problem with my jars every once in a while.. after I pressure cook the jars and they are sterile what are some ways the bacteria can get in there? Ive been doing G2G transfers with rye grains in a glove box.. I wipe down every jar with the highest % alcohol I can buy and spray the air with lyscol before I put them into the glovebox. I even have alcohol spray inside the glovebox to be even more sterile... It smells like alcohol so bad in there when I open it up I don't know how any bacteria could get in there?
What is the main cause of this bacteria? after hydrating beens making sure the grains are dry? thats the only thing I can think of since the grains get so wet when the bacteria is ther.. but even then I don't have this bacteria problem until after the G2G transfer...
I have a jar sitting on my floor right now that I sterilized back in december or maybe early early january that looks mildly dehydrated but appears to me to be in fine condition. There isn't any presence of bacteria or mold that I can see. It's been a week or so since I've observed it but I doubt much has changed since then.
Spraying alcohol inside of your glove box can cause "fresh" (read:dirty) air to be sucked into it. Soap and water will be fine.
There can also be a greater number of instances of bacterial contamination if you over-hydrate your rye grains (or rather don't allow them to dry sufficiently after hydrating).
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thatoneguy211
chillin like a villian


Registered: 06/04/08
Posts: 106
Last seen: 10 years, 11 months
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Re: Its bacteria right? [Re: PulseEden]
#14267983 - 04/10/11 05:09 PM (12 years, 9 months ago) |
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Quote:
PulseEden said: If you have a glass fishtank you don't use make a glove box out of that. Get a piece of plexi glass, cut it to fit the top of the tank and then cut two holes in it for your arms that are no more than a half inch wider than the widest part of your forearms. Lay the plexi ontop of the tank, duct tape it off all around the edges. Eliminates the suction issue from bumping your glovebox.
Keep in mind that opening this up is annoying, so if you're going to have to put things in it that won't fit through the arm holes, make a door in the plexi glass to the side for bigger stuff.
Good idea, Im going to make this right now from my old fishtank! Ive been using a clear tubberware which has worked fine, but I like this setup better!
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Deej3987
Deej3987

Registered: 12/08/09
Posts: 128
Last seen: 1 year, 5 months
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Re: Its bacteria right? [Re: Humility]
#14278403 - 04/12/11 02:45 PM (12 years, 9 months ago) |
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Alcohol being sprayed inside of the GB makes dirty air get into it? how?
-------------------- My Trade List Printing All info I post about myself, what I am doing, and what I have done is fake; and therefore, holds no truth and can not be used to prove any previous action of mine.
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Humility
Working on it



Registered: 10/07/08
Posts: 6,745
Last seen: 6 years, 11 months
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Re: Its bacteria right? [Re: Deej3987]
#14278432 - 04/12/11 02:50 PM (12 years, 9 months ago) |
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Diffusion my good man
Diffusion.
--------------------

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Deej3987
Deej3987

Registered: 12/08/09
Posts: 128
Last seen: 1 year, 5 months
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Re: Its bacteria right? [Re: Humility]
#14278525 - 04/12/11 03:04 PM (12 years, 9 months ago) |
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so basically alcohol being sprayed inside of the GB is a bad idea? it does not help prevent contams¿?
-------------------- My Trade List Printing All info I post about myself, what I am doing, and what I have done is fake; and therefore, holds no truth and can not be used to prove any previous action of mine.
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PulseEden
Cyborg



Registered: 01/13/10
Posts: 140
Last seen: 11 years, 11 months
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Re: Its bacteria right? [Re: Deej3987]
#14290850 - 04/14/11 03:41 PM (12 years, 9 months ago) |
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soak a paper towel in alcohol (no dry spots on the towel, these can carry contaminates) wipe down all of the insides. Works better than spraying imo.
-------------------- I booped your daughter! A boop boop boop!
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Deej3987
Deej3987

Registered: 12/08/09
Posts: 128
Last seen: 1 year, 5 months
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Re: Its bacteria right? [Re: PulseEden]
#14290989 - 04/14/11 04:03 PM (12 years, 9 months ago) |
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Thanks for the advice I will remember that next time I'm doing anything.
-------------------- My Trade List Printing All info I post about myself, what I am doing, and what I have done is fake; and therefore, holds no truth and can not be used to prove any previous action of mine.
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Humility
Working on it



Registered: 10/07/08
Posts: 6,745
Last seen: 6 years, 11 months
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Re: Its bacteria right? [Re: Deej3987]
#14291258 - 04/14/11 04:45 PM (12 years, 9 months ago) |
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Contaminants will always be present even in a sealed room with a HEPA machine running 24/7; the idea is to get the concentrations low enough so that they don't bother our work or to in some other way cause them to not interfere with the work.
A glove box is in effect a "Still air chamber". The air inside of the glove box is fairly undisturbed relative to the air outside. Particles bounce off the box and the air inside has a relatively low concentration of particles bouncing around which can land in a jar and cause contamination.
Alcohol won't affect airborne contaminants, at all, so don't use it. Surface contaminants can be wiped away with soap and water.
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