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tarfoh
Friend



Registered: 11/27/10
Posts: 126
Last seen: 4 years, 11 months
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Fastest method for growing on WBS?
#14208196 - 03/30/11 08:01 AM (12 years, 9 months ago) |
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Hey all. So I am sad to report that due to a complicated series of events, I was forced to throw away all of my LC's. What I am trying to do now is quickly colonize some myco bags that will be filled with about 2-3 quarts of WBS. Now my question is this: What is the fastest way to colonize these myco bags? Should I:
1) inoculate the bags with a standard spore syringe?
2) inoculate the spores into an LC solution, wait for it to grow out, then use the new LC to inoculate the bags?
3) Drop some spores on agar and then take a wedge and either drop it into an LC or drop it into the myco bags?
What do you guys think? Thanks.
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anonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
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Re: Fastest method for growing on WBS? [Re: tarfoh]
#14208211 - 03/30/11 08:06 AM (12 years, 9 months ago) |
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I'm finding grain to grain to be ridiculously fast. You could put spores to agar, isolate, then from agar make master grain jars for g2g.
It's faster and more reliable than LC.
-------------------- The above post is fictional, hypothetical, or downright nonsensical.
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SWIM Jr
SWIM SWAM

Registered: 02/14/11
Posts: 131
Loc: In The Pool
Last seen: 11 years, 2 months
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Re: Fastest method for growing on WBS? [Re: anonjon]
#14208305 - 03/30/11 08:36 AM (12 years, 9 months ago) |
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If you have a syringe, you could do all three!
-------------------- I am mighty tighty whitey, and I am smuggling grapes.
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tarfoh
Friend



Registered: 11/27/10
Posts: 126
Last seen: 4 years, 11 months
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Re: Fastest method for growing on WBS? [Re: SWIM Jr]
#14208317 - 03/30/11 08:41 AM (12 years, 9 months ago) |
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This is true, however I am quite sure that doing all three would not be the most efficient use of time.
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biologys
Mycologist in Trainning




Registered: 12/21/09
Posts: 4,622
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Re: Fastest method for growing on WBS? [Re: tarfoh]
#14208328 - 03/30/11 08:44 AM (12 years, 9 months ago) |
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since you're in a hurry, why dont u..put a drop on agar, use that to clean up, and do a LC for future use.
and use the remainder of the syringe to nocc up your jars...
then ya can do G2G, and us LC to start other jars, and not have to wait for spore germination, and all of that...
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Fastest method for growing on WBS? [Re: anonjon]
#14209231 - 03/30/11 12:05 PM (12 years, 9 months ago) |
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Quote:
anonjon said: I'm finding grain to grain to be ridiculously fast. You could put spores to agar, isolate, then from agar make master grain jars for g2g.
It's faster and more reliable than LC.
This.
-------------------- You make it all possible. Doesn't it feel good?
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