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Offlinemellowparty
legitimate researcher


Registered: 05/17/09
Posts: 18,467
Last seen: 10 years, 2 months
Re: Cambodian 40x Microscopy [Re: singult]
    #14348628 - 04/25/11 11:28 AM (12 years, 9 months ago)

Quote:

, I am used to just saying 40x around my lab... And yes, it has oil immersion



Is it a 40x (plan)apochromat?


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OfflineBstsneverr
Peace
Male


Registered: 03/22/08
Posts: 480
Last seen: 1 year, 9 months
Re: Cambodian 40x Microscopy [Re: mellowparty]
    #14349251 - 04/25/11 02:07 PM (12 years, 9 months ago)

:popcorn:


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*** Psalm 9:9-10 ***

The lord is a refuge for the oppressed, a stronghold in times of trouble.

Those who know your name will trust in you, for you, lord, have not forsaken those who seek you.


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InvisibleAlkaloids
3,4,5-trimethoxyphenethylamine
Male


Registered: 11/15/98
Posts: 743
Loc: pubis mons
Re: Cambodian 40x Microscopy [Re: Bstsneverr]
    #14368529 - 04/28/11 06:13 PM (12 years, 9 months ago)

Great microscopy pics Hags.  :smile:  I'm just chiming in.  No access to a microscope currently. Thanks for posting


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OfflineHags
The Scientist


Registered: 08/22/10
Posts: 48
Last seen: 12 years, 5 months
Re: Cambodian 40x Microscopy [Re: Alkaloids]
    #14396294 - 05/03/11 07:32 PM (12 years, 8 months ago)

mellowparty: its a normal microscope objective, not photo lens... think thats what you were asking...?

Bad news, ran the spores in the fluorescent scope. No autofluorescence at all, went from wavelengths 400-700 (DAPI/FITC/TRITC/mCHERRY)

Super sad, not that it would have validated anything scientifically other than simple autofluorescence, but it would have looked damn cool!


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OfflineAlan RockefellerM
Mycologist
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Registered: 03/10/07
Posts: 48,276
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Re: Cambodian 40x Microscopy [Re: Hags]
    #14396367 - 05/03/11 07:44 PM (12 years, 8 months ago)

Quote:

Hags said:
Bad news, ran the spores in the fluorescent scope. No autofluorescence at all, went from wavelengths 400-700 (DAPI/FITC/TRITC/mCHERRY)





Try the gill edges and other parts of the cap.


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OfflineHags
The Scientist


Registered: 08/22/10
Posts: 48
Last seen: 12 years, 5 months
Re: Cambodian 40x Microscopy [Re: Alan Rockefeller]
    #14396458 - 05/03/11 07:58 PM (12 years, 8 months ago)

brilliant idea AR, will do! :mushroom2:


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Offlinemellowparty
legitimate researcher


Registered: 05/17/09
Posts: 18,467
Last seen: 10 years, 2 months
Re: Cambodian 40x Microscopy [Re: Hags]
    #14398926 - 05/04/11 09:00 AM (12 years, 8 months ago)

Quote:

Hags said:
mellowparty: its a normal microscope objective, not photo lens... think thats what you were asking...?

Bad news, ran the spores in the fluorescent scope. No autofluorescence at all, went from wavelengths 400-700 (DAPI/FITC/TRITC/mCHERRY)

Super sad, not that it would have validated anything scientifically other than simple autofluorescence, but it would have looked damn cool!




When you said that it has oil immersion were you referring to the 40x objective or to the microscope in general. A 40x obj. with immersion would have N.A. way above the standard 0.65 for achomats (at least the oldies :grin:). But u were prolly talking about a different objective anyway :stoned:

Do you think you could stain the spores with DAPI and/or calcaflour. That would be uber win to spot the spore nucleus shining in blue :yesnod:


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Offlinepsylo-not
Stranger
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Registered: 04/27/11
Posts: 306
Last seen: 1 year, 1 day
Re: Cambodian 40x Microscopy [Re: mellowparty]
    #14486834 - 05/20/11 09:38 PM (12 years, 8 months ago)

pfffttftft... Scientists make me feel dumb... :blush:

Without the pictures you guys lost me a while back, I couldn't even imagine trippin with you...

Cool pics though :smile: Just tried some cambos so was neat to see up close.


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InvisibleDork
( . )( . )
 User Gallery


Registered: 02/20/11
Posts: 4,339
Re: Cambodian 400x Microscopy *DELETED* [Re: Hags]
    #14487648 - 05/21/11 12:16 AM (12 years, 8 months ago)

Post deleted by consumesomeshrooms

Reason for deletion: frogs



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Offlinemellowparty
legitimate researcher


Registered: 05/17/09
Posts: 18,467
Last seen: 10 years, 2 months
Re: Cambodian 400x Microscopy [Re: Dork]
    #14488367 - 05/21/11 04:43 AM (12 years, 8 months ago)

Hm these are not mammalian, are they? Amphibian maybe?

I see a lot of diffraction artefacts.

Here are some basidiospores at 40x10 mag.



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