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OfflineThisIsPathetic
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Experimental Liquid Culture TEK? Possibly a new ingredient...
    #14204146 - 03/29/11 02:49 PM (12 years, 9 months ago)

Has anyone EVER tried this...

For the additives with Liquid Culture... What if you used "Orange Juice"?

I tried searching on the forums for anyone who has ever thought about this... but the only posts that come up have to do with Vitamin C and such...

My Logic? I remember reading up when someone (I think it was RR) noticed that his coffee pot quickly grew mold on it when he left it out... which birthed the idea of using 'coffee grounds' as an additive in a TEK...

I haven't done too much research about using like 'coffee water' or anything like that with LCs... but I did notice that if you leave out a glass of orange juice... it quickly starts to grow mold... So it gave me the idea that it MIGHT work in some kind of TEK for growing...


Possible Issues? I also know that the mold that grows on orange juice is white... so it might be REALLY hard to tell if it works or not... due to the color of mycelium and such being white as well... but not 100% sure...

Thinking about trying it out and doing a grow log for it... Ideas on that?

Has anyone ever thought about it, or tried it out?

Think it's interesting enough to do a log of it?


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Offlinestranger_danger
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Re: Experimental Liquid Culture TEK? Possibly a new ingredient... [Re: ThisIsPathetic]
    #14204166 - 03/29/11 02:53 PM (12 years, 9 months ago)

only real issue i can think of is the acidity... it could possibly hurt the mycelium i would think


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OfflineThisIsPathetic
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Re: Experimental Liquid Culture TEK? Possibly a new ingredient... [Re: stranger_danger]
    #14204354 - 03/29/11 03:27 PM (12 years, 9 months ago)

Quote:

stranger_danger said:
only real issue i can think of is the acidity... it could possibly hurt the mycelium i would think




that is true... I wonder if there is something else you could add to bring the 'acid' levels down that wouldn't harm the mycelium... or I wonder 'what it is' in orange juice that causes mold to grow o.O

Good insight tho :thumbup:


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Invisiblemycoelf
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Re: Experimental Liquid Culture TEK? Possibly a new ingredient... [Re: ThisIsPathetic]
    #14204721 - 03/29/11 04:54 PM (12 years, 9 months ago)

mold in OJ would be a selected acidophiles, consuming the sugar in a niche that other mold can not grow in. PH, the power o' hydrogen, of course a consideration. If you wish to pursue the best course would be to pour some agar at different concentrations and subculture from the same mother, finding thresholds, establishing base line etc. Don't forget your control.

You never know, but the subject of substrate has been beaten to death, for further enlightenment may I suggest you check out growing gourmet and medicinal mushrooms, by Paul stametes:goodluck:


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Mycoelf

Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable  the goal of infinity becomes.  Remember, cleanliness in next to goddessness

:aliendance::aliendance::wicca::aliendance::aliendance::pipesmoke:


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OfflineThisIsPathetic
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Re: Experimental Liquid Culture TEK? Possibly a new ingredient... [Re: mycoelf]
    #14204899 - 03/29/11 05:26 PM (12 years, 9 months ago)

Quote:

mycoelf said:
mold in OJ would be a selected acidophiles, consuming the sugar in a niche that other mold can not grow in. PH, the power o' hydrogen, of course a consideration. If you wish to pursue the best course would be to pour some agar at different concentrations and subculture from the same mother, finding thresholds, establishing base line etc. Don't forget your control.

You never know, but the subject of substrate has been beaten to death, for further enlightenment may I suggest you check out growing gourmet and medicinal mushrooms, by Paul stametes:goodluck:




Damn dude... I think I had to Google like at least 5 words in that post HAHA :laugh: Think you could elaborate a little bit more? I'm not sure what agar would have to do with LC... or even substrate?

I was thinking more of something like 'a quart of water' with 'x' amount of OJ added to it... pressure cooked, and then injected with spores...


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Offlinestranger_danger
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Re: Experimental Liquid Culture TEK? Possibly a new ingredient... [Re: ThisIsPathetic]
    #14205493 - 03/29/11 07:26 PM (12 years, 9 months ago)

i really think you should just stick with honey, dextrose of some sort, malt extract, etc etc etc.

but who knowS? try it and let us know what happens


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OfflineThisIsPathetic
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Re: Experimental Liquid Culture TEK? Possibly a new ingredient... [Re: stranger_danger]
    #14205699 - 03/29/11 08:06 PM (12 years, 9 months ago)

Quote:

stranger_danger said:
i really think you should just stick with honey, dextrose of some sort, malt extract, etc etc etc.




yaa, I do, this was just a bit of my curiosity :P maybe find out something AWESOME that was never known before haha

most def will :smile:


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Invisiblemycoelf
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Re: Experimental Liquid Culture TEK? Possibly a new ingredient... [Re: ThisIsPathetic]
    #14208697 - 03/30/11 10:24 AM (12 years, 9 months ago)

My point here was to say that if yo want to know what effect a substance will have on a myc then you pour agar with X substance in the agar at varying concentration, then subculture onto all agar from the same mother culture and observe the effects, all else being the same.

Agar is 2 dimensional,= easy to see the effects, if any of said substance. If you are talking about doing performance trials in G1 or lc or what ever, yo have vastly complicated the measurement of your results.

Everyone wants to discover and contribute to this science, I sure as hell did, but I figure the only way I have to get there is to thoroughly understand all of the prior art. That is why I am here. There are folks on here that are so experienced and knowledgeable that standing next to I am a total newb. I am here to learn as much as I can and teach where I can. Stametes books is highly recommended as a first read in this path. Most of everything is in there as fundamentals go, but in the ten or so years GGMM has been out there has been alot of new info (tricks) learned by the collective that is not publish. You just can not print fast enough to keep up with the current thinking. That is why we are luck to have the shroomery :goodluck::headbang:


--------------------
Mycoelf

Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable  the goal of infinity becomes.  Remember, cleanliness in next to goddessness

:aliendance::aliendance::wicca::aliendance::aliendance::pipesmoke:


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InvisibleCheeWiz
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Re: Experimental Liquid Culture TEK? Possibly a new ingredient... [Re: ThisIsPathetic]
    #14208807 - 03/30/11 10:48 AM (12 years, 9 months ago)

Hi all; almost all fungi favor a more acid environment to grow in. This is because they have evolved as secondary decomposers. That is as the much faster growing bacteria are doing their thing the waste they produce is acidic. In time it will start to inhibit their growth leaving an opportunity for the slower growing fungi to come in and do their thing. Even thou many fungi have developed enzyme systems to digest things like straw and wood they still have that primordial evolution in their genes.

It has been an old trick in mycology going back some hundred years to use a medium that’s somewhat on the acidic side of around 5.2 to 5.8 PH as most bacteria do poorly at these PHs; that again is most and not all. Most of the older mediums used in mycology are naturally on the acidic side; thou they may not be quite as low as 5.2. The down side is that the metabolites from the growing mycelium will in time lower the PH even more and when you start to get bellow 5 you start to inhibit the growth of many fungi. So if you want to start to lower the PH of your LC one might want to think about using a buffer to lock that PH in. As with many things in life as you start to hit the optimal level the inverses curve is usually not fair away and the drop from that curve can be more of an avalanche than the gradual slope getting there.

To lower the PH of an agar or LC all one needs is some PH strips with the proper PH range, distilled vinegar and some way of adding the vinegar in a controlled way. Test your medium PH with the test strips and add the vinegar little at a time till you reach the PH you want; for me it's 5.6. Again the use of a phosphate buffer that's for the PH you want is helpful in LC that going to be held for a time; like storing in the cooler to inoculate spawn jars with over time.


Edited by CheeWiz (03/30/11 07:06 PM)


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OfflineRogerRabbitM
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Re: Experimental Liquid Culture TEK? Possibly a new ingredient... [Re: CheeWiz]
    #14212246 - 03/30/11 09:50 PM (12 years, 9 months ago)

Quote:

For the additives with Liquid Culture... What if you used "Orange Juice"?




I know it won't hurt.  Whether it helps or not remains for experimentation.  I always adjust the pH of my well water down with vinegar, but if I run out, I get Mrs Rabbit's lemon juice concentrate out of the refrigerator and use it to get the pH down to 5 when making substrates.  Both perform equally, but vinegar is much less expensive.  I've been meaning to price a 55 gallon drum of citric acid, but haven't gotten around to it yet.
RR


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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Offlineshroomie_glen
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Re: Experimental Liquid Culture TEK? Possibly a new ingredient... [Re: RogerRabbit]
    #14214399 - 03/31/11 11:39 AM (12 years, 9 months ago)

Do it and report back././.././....
FOR SCIENCE.


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OfflineThisIsPathetic
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Re: Experimental Liquid Culture TEK? Possibly a new ingredient... [Re: shroomie_glen]
    #14214785 - 03/31/11 01:09 PM (12 years, 9 months ago)

Quote:

shroomie_glen said:
Do it and report back././.././....
FOR SCIENCE.




IM DOING SCIENCE SOO HARD RIGHT NOW.


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OfflineMycelio
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Re: Experimental Liquid Culture TEK? Possibly a new ingredient... [Re: ThisIsPathetic]
    #14215240 - 03/31/11 02:45 PM (12 years, 9 months ago)

There is lots of information available on using all kinds of fruit juices in submerged fermentation, incl. orange, apple and V8. Just perform a web search.

Carsten


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