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OfflinefungalEntropy
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First time at WBS monotubs, tell me how they look
    #14203309 - 03/29/11 11:39 AM (12 years, 9 months ago)

This is my first attempt at monotubs (well actually mini mono I followed docTs mini mono tub tek)  Its been 10 days since I mixed in the coir with the spawn.  There are a few things I'm concerned about, but I need your guys opinion.  Just tell me how they're looking and what I need to do next.  I don't think there's any contams going on but I'm unaware for what to look for.  Here are some pics.



Tell me if you see anything wrong here and if not what I should do next
(like for example if you click on the pics you can get a better look and actually then zoom in)  If you notice on the less colonized tub the offwhite color puff in the corner I don't know if that is normal and then in the more colonized tub in the top left corner there is some yellowing which I'm hoping is metabolites) Again, just let me know if I should procede and how to do so.  Thanks everyone in advance for your help


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Invisibleanonjon
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Re: First time at WBS monotubs, tell me how they look [Re: fungalEntropy]
    #14203343 - 03/29/11 11:46 AM (12 years, 9 months ago)

A little yellowing around the edges from drying is normal. That looks good. You should see knots any day now.

If the yellowing worsens it could mean the tub is evaporating out a little too fast or the light is too hot/close.

What's the ambient humidity?

This one looks a little suspicious, but even if it's contamn, doesn't look fatal.


--------------------
The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:


Edited by anonjon (03/29/11 11:49 AM)


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InvisibleSillicybin
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Re: First time at WBS monotubs, tell me how they look [Re: fungalEntropy]
    #14203347 - 03/29/11 11:47 AM (12 years, 9 months ago)

They look excellent.  You just need to keep doing exactly what you've been doing and let the colonization finish.  The yellow is metabolites - it is normal.  Just let it keep doing what it's doing for now, and you'll be sitting pretty.  After they've fully colonized, give it a few more days after that, THEN start misting and fanning.


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OfflineProfessorPinHead
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Re: First time at WBS monotubs, tell me how they look [Re: fungalEntropy]
    #14203348 - 03/29/11 11:47 AM (12 years, 9 months ago)

looks good :thumbup:


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Offlinenotguilty
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Re: First time at WBS monotubs, tell me how they look [Re: Sillicybin]
    #14203367 - 03/29/11 11:52 AM (12 years, 9 months ago)

:winner:


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OfflinefungalEntropy
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Re: First time at WBS monotubs, tell me how they look [Re: ProfessorPinHead]
    #14203370 - 03/29/11 11:52 AM (12 years, 9 months ago)

Awesome news you guys, that just made my day I like got chills when I read your comments.  Should I be misting in the tub.  I followed docTs minimonotub tek and u know there are 1/4inch holes every 2 inches, I don't have them under lights yet.  I know for sure to let the unfinished tub to keep going but the other one should I put it into fruiting?  It's PE btw and I've been dying to try some ever since I heard that people said they were way more potent so I want to find out for myself :wink:  Thanks again guys I was worried for a second but you all just made my day for sure *bows head*


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OfflinefungalEntropy
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Re: First time at WBS monotubs, tell me how they look [Re: anonjon]
    #14203380 - 03/29/11 11:55 AM (12 years, 9 months ago)

Quote:

anonjon said:
A little yellowing around the edges from drying is normal. That looks good. You should see knots any day now.

If the yellowing worsens it could mean the tub is evaporating out a little too fast or the light is too hot/close.

What's the ambient humidity?

This one looks a little suspicious, but even if it's contamn, doesn't look fatal.





If your talking about those brown specs?  I think those are just little bits of coir or u mean something else?

Oh and one more question, what do I look for to tell when it's ready to throw into fruiting mode? knots?  pins starting to forM?


Edited by fungalEntropy (03/29/11 11:57 AM)


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OfflinefungalEntropy
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Re: First time at WBS monotubs, tell me how they look [Re: fungalEntropy]
    #14203460 - 03/29/11 12:16 PM (12 years, 9 months ago)

bump


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Invisibleanonjon
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Re: First time at WBS monotubs, tell me how they look [Re: fungalEntropy]
    #14203565 - 03/29/11 12:45 PM (12 years, 9 months ago)

There's a beige spot in the center there and a smaller one just northeast of it.

The smaller one has three little spheres in it.

It could be nothing, or it could be some trich under there, or cinnamon mold, or bacteria.

Here's an example of bacterial contamn:

See the little round drops of fluid oozing from that growth?

Could be fine tho. The rest of the thing looks great.


--------------------
The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:


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InvisibleDJYoshaBYD
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Re: First time at WBS monotubs, tell me how they look [Re: anonjon]
    #14203585 - 03/29/11 12:49 PM (12 years, 9 months ago)

it looks good to me.. nice job! cant wait to see the flush


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OfflinefungalEntropy
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Re: First time at WBS monotubs, tell me how they look [Re: DJYoshaBYD]
    #14205527 - 03/29/11 07:33 PM (12 years, 9 months ago)

Should I be misting at all? or is that when I start fruiting stage?  Also when do I know to start the tub in fruiting conditions?

Thanks again for all the help


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InvisibleM11
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Re: First time at WBS monotubs, tell me how they look [Re: fungalEntropy]
    #14205636 - 03/29/11 07:53 PM (12 years, 9 months ago)

Did you just take those photos after waiting 10 days after spawning?


--------------------
Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter.  Just pretend it's christmas.
-RR

Those mushrooms are fine.  Your friend is a pussy.
-RR

Outdoor Patch


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OfflinefungalEntropy
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Re: First time at WBS monotubs, tell me how they look [Re: M11]
    #14205664 - 03/29/11 07:59 PM (12 years, 9 months ago)

Quote:

M11 said:
Did you just take those photos after waiting 10 days after spawning?




Yes, precicely


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Offlineafrosheen
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Re: First time at WBS monotubs, tell me how they look [Re: fungalEntropy]
    #14205698 - 03/29/11 08:06 PM (12 years, 9 months ago)

Quote:

fungalEntropy said:
Quote:

M11 said:
Did you just take those photos after waiting 10 days after spawning?




Yes, precicely




That's excellent. I'd say it's ready for fruiting conditions. If there's a dew on top of the myc, don't mist until that evaporates. The fresh air it'll be getting should evaporate it fairly quickly, and will trigger pinning.


--------------------


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InvisibleM11
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Re: First time at WBS monotubs, tell me how they look [Re: afrosheen]
    #14205724 - 03/29/11 08:09 PM (12 years, 9 months ago)

I would consolidate for a week before fruiting.


--------------------
Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter.  Just pretend it's christmas.
-RR

Those mushrooms are fine.  Your friend is a pussy.
-RR

Outdoor Patch


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Offlineafrosheen
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Re: First time at WBS monotubs, tell me how they look [Re: M11]
    #14205745 - 03/29/11 08:13 PM (12 years, 9 months ago)

Quote:

M11 said:
I would consolidate for a week before fruiting.




Not a bad idea either. It never hurts to wait once it gets to this point, the substrate can only get stronger.


--------------------


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OfflinefungalEntropy
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Re: First time at WBS monotubs, tell me how they look [Re: afrosheen]
    #14205948 - 03/29/11 08:46 PM (12 years, 9 months ago)

Sweet, I'll give it about a week till I get it into fruiting conditions, thanks guys for the help


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InvisibleDoc_T
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Re: First time at WBS monotubs, tell me how they look [Re: fungalEntropy]
    #14207728 - 03/30/11 03:30 AM (12 years, 9 months ago)

Quote:

fungalEntropy said:
Should I be misting at all? or is that when I start fruiting stage?  Also when do I know to start the tub in fruiting conditions?

Thanks again for all the help




You will rarely need to mist during colonization, but you can if needed.
I cover the tub with a bag (not sealed) to prevent drying.

During fruiting, you spray rather than mist. Don't be a pussy, spray the block down good- then let it evaporate.
Let it get a bit dry, then re-wet it to capacity about once a day or so, as needed.


--------------------
You make it all possible. Doesn't it feel good?


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OfflineProfessorPinHead
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Re: First time at WBS monotubs, tell me how they look [Re: Doc_T]
    #14207766 - 03/30/11 03:50 AM (12 years, 9 months ago)

Quote:

Doc_T said:
During fruiting, you spray rather than mist. Don't be a pussy, spray the block down good- then let it evaporate.
Let it get a bit dry, then re-wet it to capacity about once a day or so, as needed.




:thumbup:


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InvisibleDoc_T
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Re: First time at WBS monotubs, tell me how they look [Re: ProfessorPinHead]
    #14207778 - 03/30/11 03:54 AM (12 years, 9 months ago)

Depending on conditions, you may not need to mist at all, or every few days.
But most people will, especially if there's a furnace going. In fact you may need to mist mornings and evenings if so.
But not all day- humidity comes from the substrate evaporation, you don't provide it by misting. Wet it and forget it.


--------------------
You make it all possible. Doesn't it feel good?


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InvisibleM11
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Re: First time at WBS monotubs, tell me how they look [Re: Doc_T]
    #14208775 - 03/30/11 10:42 AM (12 years, 9 months ago)

Quote:

Doc_T said:
Quote:

fungalEntropy said:
Should I be misting at all? or is that when I start fruiting stage?  Also when do I know to start the tub in fruiting conditions?

Thanks again for all the help




You will rarely need to mist during colonization, but you can if needed.
I cover the tub with a bag (not sealed) to prevent drying.

During fruiting, you spray rather than mist. Don't be a pussy, spray the block down good- then let it evaporate.
Let it get a bit dry, then re-wet it to capacity about once a day or so, as needed.




So if you are using wax paper as a casing, you remove it, spray the substrate surface, then replace it?  How would the substrate surface effectively "dry" if you are using a wax paper casing?


--------------------
Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter.  Just pretend it's christmas.
-RR

Those mushrooms are fine.  Your friend is a pussy.
-RR

Outdoor Patch


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Invisiblebiologys
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Re: First time at WBS monotubs, tell me how they look [Re: M11]
    #14208968 - 03/30/11 11:19 AM (12 years, 9 months ago)

you dont need to mist with the wax paper, and you replace the wax paper every couple of days, and remove once pinset comes in.


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InvisibleM11
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Re: First time at WBS monotubs, tell me how they look [Re: biologys]
    #14209532 - 03/30/11 01:15 PM (12 years, 9 months ago)

I have heard of people using it through fruit development.  I only use mine until the pin set comes in.


--------------------
Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter.  Just pretend it's christmas.
-RR

Those mushrooms are fine.  Your friend is a pussy.
-RR

Outdoor Patch


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OfflinefungalEntropy
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Re: First time at WBS monotubs, tell me how they look [Re: M11]
    #14224472 - 04/02/11 01:13 AM (12 years, 9 months ago)

UPDATE!!!

there is dew all over the surface of the substrate "block" and little knots are forming, here are some new pics, I am a litttle concerned about this yellowing forming in different areas of the tub.  They are mainly around the edges.  Anything to worry about?  If not I just got my lighting wired up and I can put the tub in fruiting conditions at any given moment.  Just still wondering about this coloration on the substrate.  Here are the pics



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Invisiblebiologys
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Re: First time at WBS monotubs, tell me how they look [Re: fungalEntropy]
    #14224484 - 04/02/11 01:17 AM (12 years, 9 months ago)

myc secretes metabolites to break down the substrate so it can consume it, no worries fruit that thing..


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OfflinefungalEntropy
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Re: First time at WBS monotubs, tell me how they look [Re: biologys]
    #14224504 - 04/02/11 01:22 AM (12 years, 9 months ago)

So your 100% on those are metabolites in the pics and I should put that baby under this 6500k 15 watt CFL bulb right now?!?!  Say the word and she's underway


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Invisiblebiologys
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Re: First time at WBS monotubs, tell me how they look [Re: fungalEntropy]
    #14224512 - 04/02/11 01:24 AM (12 years, 9 months ago)

yep, definately metabolites..and ready to be fruited.. give it light and FAE


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OfflinefungalEntropy
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Re: First time at WBS monotubs, tell me how they look [Re: biologys]
    #14224526 - 04/02/11 01:28 AM (12 years, 9 months ago)

Awesome, thanks for the help still new at this and first time doing tubs so I'm excited will keep updated as the weeks go by :laugh:  Shout outs to everyone that helped


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OfflineProfessorPinHead
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Re: First time at WBS monotubs, tell me how they look [Re: biologys]
    #14224796 - 04/02/11 04:18 AM (12 years, 9 months ago)

Quote:

biologys said:
myc secretes metabolites to break down the substrate so it can consume it, no worries fruit that thing..



:thumbup:


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OfflinefungalEntropy
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Re: First time at WBS monotubs, tell me how they look [Re: ProfessorPinHead]
    #14295019 - 04/15/11 11:04 AM (12 years, 9 months ago)

UPDATE: TRICH!?!?!

Here are some recent pics just taken a couple of minutes ago.  I look in the tub today and there are green fuzzy spots on the substrate....  I just don't know how the tub goes from being fully colonized (built up immunity from contams) then just BAM trich?  It's been in fruiting conditions for about a week now tell me your opinions here are some pics



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OfflinefungalEntropy
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Re: First time at WBS monotubs, tell me how they look [Re: fungalEntropy]
    #14295112 - 04/15/11 11:26 AM (12 years, 9 months ago)

*Bump!*

I need to know if this tub needs to go! asap I don't know if it's trich but if it is I don't want to hangin out in my house.


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InvisibleM11
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Re: First time at WBS monotubs, tell me how they look [Re: fungalEntropy]
    #14295520 - 04/15/11 12:59 PM (12 years, 9 months ago)

That spot looks suspicious, but I would hang on to it a little longer.  Keep an eye on it.  If any signs of green show -toss immediately.


--------------------
Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter.  Just pretend it's christmas.
-RR

Those mushrooms are fine.  Your friend is a pussy.
-RR

Outdoor Patch


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Offlineafrosheen
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Re: First time at WBS monotubs, tell me how they look [Re: M11]
    #14300096 - 04/16/11 09:06 AM (12 years, 9 months ago)

Looks like bruising to me, keep on truckin'.


--------------------


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OfflinefungalEntropy
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Re: First time at WBS monotubs, tell me how they look [Re: afrosheen]
    #14300137 - 04/16/11 09:21 AM (12 years, 9 months ago)

u shure that looks like bruising?  I know psicocybes can bruise green as I had them do it before but why would it be bruising.  I don't think I touched it much but one time one of those stupid asian beetles found it's way into my box and it was a struggle to get it out, can the substrate bruise from lack of water or something.  I got it under 12/12 light cycle and it was fully colonized before I fruited it.  Which is why I am confused to see these unhealthy looking spots on my sub.  How will I know for sure if it's trich?  Is there any smell associated with it and what does it look like exactly?  What do you think I should do?


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Offlineafrosheen
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Re: First time at WBS monotubs, tell me how they look [Re: fungalEntropy]
    #14300205 - 04/16/11 09:48 AM (12 years, 9 months ago)

Doesn't look like a trich pattern at all. What you're seeing is subsurface bruising caused by drying or misting.

It's common to have a nice, fluffy upper layer of aerial mycelium poking up over the entire surface, then you mist a few times, it dries a few times, and becomes this strange layer that looks like a latex coating. That's what I'm seeing here. As that coating forms and collapses, there may be ridges of aerial mycelium getting squashed underneath it, which will bruise. It's natural and nothing to worry about.

Trust me, if you were to see trich forming, it'd be super white puffs first (separate or joined) that grew too fast. Trich "sticks together" generally and will show up in an isolated area to begin with. As it matures it'll turn dark green during the spore production phase. It will also be on the outside of the substrate, not hidden under the skin.


--------------------


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OfflinefungalEntropy
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Re: First time at WBS monotubs, tell me how they look [Re: afrosheen]
    #14300644 - 04/16/11 12:27 PM (12 years, 9 months ago)

For sure man, I do hope it is just bruising, as the day progresses the green spots are spreading.  About that latexy surface yeah it turned that way after I sprayed it for the first time that's normal though that's good.  Yah man I've never seen this green shit before, except when I picked em before so that's why I'm trippin on this.  I'll post another couple pics tomorrow and hopefully we're still golden.  When should pins start showin up?  This puppies been in fruiting mode for over a week now.


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Re: First time at WBS monotubs, tell me how they look [Re: fungalEntropy]
    #14300898 - 04/16/11 01:16 PM (12 years, 9 months ago)

spreading of green spots usually isnt good but good luck.  stay golden mushyboy


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InvisibleFungal growth
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Re: First time at WBS monotubs, tell me how they look [Re: drosmoka]
    #14300972 - 04/16/11 01:25 PM (12 years, 9 months ago)

i always wondered why it turned to that 'latexy surface'.
thanks for clearing that up!


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OfflinefungalEntropy
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Re: First time at WBS monotubs, tell me how they look [Re: Fungal growth]
    #14301317 - 04/16/11 02:14 PM (12 years, 9 months ago)

yo you guys say trich is dark green and starts as white puff balls in one specific area then expands from that starting point?  I googled images of trichoderma mold and it does look very dark green.  The green in my tub is a lighter green and they appeared at different spots on the substrate not a specific area.  There was also no white puff balls before the green showed up.  Again, I'll take some more pics tomorrow and see what everyone thinks.


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Re: First time at WBS monotubs, tell me how they look [Re: fungalEntropy]
    #14301483 - 04/16/11 02:42 PM (12 years, 9 months ago)

ime the trichy dichy starts on un- or weakly colonized
areas. i don't recall ever seeing it start on healthy myc
and grow out from there.
trich is like a white scab, that usually turns green in
the center within 24 hrs.


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OfflinefungalEntropy
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Re: First time at WBS monotubs, tell me how they look [Re: Fungal growth]
    #14301792 - 04/16/11 03:40 PM (12 years, 9 months ago)

ahhhh I see, yeah my substrate was fully colonized so I was kinda startled to see green stuff on there.  When should pins be arriving?  It's been in FConditions for about a week and a half now with no sign of them yet.


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InvisibleFungal growth
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Re: First time at WBS monotubs, tell me how they look [Re: fungalEntropy]
    #14301865 - 04/16/11 03:57 PM (12 years, 9 months ago)

:shrug: but i would think soon.
ime, fae is more important than humidity for pinning,
but you can't let it get too dry.


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Re: First time at WBS monotubs, tell me how they look [Re: Fungal growth]
    #14302162 - 04/16/11 05:07 PM (12 years, 9 months ago)

Quote:

Fungal growth said:
i always wondered why it turned to that 'latexy surface'.
thanks for clearing that up!




I don't know that I cleared it up, I still wonder why it happens and kinda don't like it. For me, it's associated with poor pinsets. For others, it may mean nothing. Seems like a nice protective coating though.


--------------------


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OfflinefungalEntropy
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Re: First time at WBS monotubs, tell me how they look [Re: afrosheen]
    #14322391 - 04/20/11 10:38 AM (12 years, 9 months ago)

yeeeuuup definately mold.  Checked the tub again today and it looks like moldy bread all over.  Instantly chucked the whole tub with no delay.  Hopefully the other tub which is almost fully colonized will go the distance without incountering our natural sworn enemy.  First time ever gettin contams :frown: little bummed but life goes on, if you don't succeed try try try again


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InvisibleSillicybin
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Re: First time at WBS monotubs, tell me how they look [Re: fungalEntropy]
    #14322715 - 04/20/11 12:04 PM (12 years, 9 months ago)

I'm sorry I saw this thread again late, but yeah this pic:


That is clearly trichoderma.  That looks nothing like bruising.

The usual cause is a contaminant that caused a small portion inside the substrate to remain uncolonized, which didn't outbreak until conditions became more favorable for it.  The change in pH (additional acidity) caused by mycelium's metabolite production can trigger a latent trich outbreak.

If you want to try using this container again, and you're absolutely certain the spawn wasn't at all infected, you could try increasing gas exchange during colonization of the bulk sub.

EDIT: Looking at your top pics again, your G/E should have been fine if those holes weren't covered.  If the bulk was properly pasteurized and hydrated, your guess is as good as mine.


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OfflinefungalEntropy
misfiring synapses


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Re: First time at WBS monotubs, tell me how they look [Re: Sillicybin]
    #14322827 - 04/20/11 12:29 PM (12 years, 9 months ago)

yah mon, it's all gravy I tossed the whole tub, sucks cuz it took a while to make (drillin all the holes and plexi window in the top) but I'd rather not risk infesting my house with any trich spores.  Anyways yeah wish I would have found out sooner but it should be ok.  I had the substrate colonizing in the tub which was in a forceflex trash bag.  I had the trash bag tied shut (don't know if I should do this or just leave the end open?) I figured it would help keep contams out but maybe it cut off circulation for GE?  Let me know if next time I should just keep the end of the bag open.  Also the substrate probably wasn't deeep enough.  It was probably 3 inches deep which is why it dried out so quick too.  Live and learn.


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InvisibleSillicybin
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Re: First time at WBS monotubs, tell me how they look [Re: fungalEntropy]
    #14323383 - 04/20/11 02:45 PM (12 years, 9 months ago)

OH, okay that explains it.

Trying skipping the trashbag altogether next time.  There's a good thread on here where some people go back and forth (well, albeit with a bit of flaming), but I'm a huge believer in the necessity of good gas exchange during spawn to bulk.  TOO much (like FAE) can cause problems, but starving the mycelium of O2 when it's trying to do a lot of work to colonize the sub will stall it out and allow anaerobic bacteria to take an upper hand.


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OfflinefungalEntropy
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Re: First time at WBS monotubs, tell me how they look [Re: Sillicybin]
    #14324108 - 04/20/11 05:25 PM (12 years, 9 months ago)

For sure man, I wonder what to do because I'm worried about other airborne contams getting in the tub because there are 1/4 inch holes every two inches (following doct's mini mono tek) I think In this case the bag is necessary but maybe i should leave it untied? The tub colonized in about a week and a half with the garbage bag tied shut the other tub I got going is deeper sub and it's still colonizing, probably going to debag that one so it can get more ge as it should Probably be done by now.


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Offlineafrosheen
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Re: First time at WBS monotubs, tell me how they look [Re: fungalEntropy]
    #14324198 - 04/20/11 05:49 PM (12 years, 9 months ago)

Quote:

fungalEntropy said:
For sure man, I wonder what to do because I'm worried about other airborne contams getting in the tub because there are 1/4 inch holes every two inches (following doct's mini mono tek) I think In this case the bag is necessary but maybe i should leave it untied? The tub colonized in about a week and a half with the garbage bag tied shut the other tub I got going is deeper sub and it's still colonizing, probably going to debag that one so it can get more ge as it should Probably be done by now.




If you're doing a mini mono you definitely want that bagged until it's 100% colonized.


--------------------


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OfflinefungalEntropy
misfiring synapses


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Re: First time at WBS monotubs, tell me how they look [Re: afrosheen]
    #14324726 - 04/20/11 07:51 PM (12 years, 9 months ago)

sweet thought so, should I leave the end tied shut or open?


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Invisibleanonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
Re: First time at WBS monotubs, tell me how they look [Re: fungalEntropy]
    #14324758 - 04/20/11 07:58 PM (12 years, 9 months ago)

If you buy an extra tub of identical size, u can just set it inside the other tub during colonization and there will be enough of a gap at the top for a little gas exchange. (pro tip)


--------------------
The above post is fictional, hypothetical, or downright nonsensical.


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