Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   Myyco.com Golden Teacher Liquid Culture For Sale

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleFloydian
veteran
Registered: 05/13/00
Posts: 1,022
what more must I do?!
    #1419863 - 03/31/03 12:48 PM (21 years, 2 days ago)

Mycelium vs. Contams ...... round 2 .......... FIGHT!

4 more jars BRF/vermiculite were started with golden teacher. Saw signs of mycelium growth within 2-3 days and then pale orange spots starting within the white about a day later. Still waiting to see if these spread but they did last time so I'm expecting the same.

Lost all 8 jars to exact same contams during last attempt. Last time jars were just boiled, so the second time around a PC was used and grower assumed that this would eliminate the problem.

So what gives? Syringes were flamed over the stove right before innoculation and jars were PC'd for over an hour. Jar lids were closed tight and syringe holes left untaped as Tek's suggest that vermiculite layer will protect from airborne contams.

All the Tek's suggest that if using a PC the sterilization would be fairly fool proof. Does grower need to get super elaborate and start spraying lysol everywhere and wearing dustmasks and what not? Or is this just bad luck or maybe contaminated syringes? Is this because holes in lid were not taped?

Recommended sterilization practices would be appreciated.


--------------------
Don't squeeze the pancake batter

Extras: Filter Print Post Top
Offlinediacamomo
Children of theVortex

Registered: 06/22/02
Posts: 619
Loc: Inside My Mind
Last seen: 15 years, 2 months
Re: what more must I do?! [Re: Floydian]
    #1419873 - 03/31/03 12:56 PM (21 years, 2 days ago)

if you are doing brf jars taped the holes after inoc. and clean the all working area with lysol ,wash your hands and use clean cloth....

DON'T give up keep working on it.... :cool:


--------------------

Edited by diacamomo (03/31/03 01:03 PM)

Extras: Filter Print Post Top
Offlinedizzymystic
beezlebub
Registered: 03/14/03
Posts: 27
Loc: the stinkbug moon
Last seen: 20 years, 3 months
Re: what more must I do?! [Re: diacamomo]
    #1419891 - 03/31/03 01:13 PM (21 years, 2 days ago)

yeh a foaf's routine after Pc'ing the Jars for 45 min is to clean his bathroom really good with like this gel bleach stuff, unclutter everything and then innoc. in the bathroom. In the bathroom there's no moving air and its cool cause my bathroom needs cleaning anyways....and like in the kitchen there's all types of food things moving around i donno, no contams yet so try it...

Extras: Filter Print Post Top
InvisibleDiploidM
Cuban

Folding@home Statistics
Registered: 01/09/03
Posts: 19,274
Loc: Rabbit Hole
Trusted Cultivator
Re: what more must I do?! [Re: Floydian]
    #1419928 - 03/31/03 01:32 PM (21 years, 2 days ago)

When my friend makes BRF jars, he tapes the holes and leaves the lid loose but covered with foil to PC. When the jars cool, he takes a shower and puts on clean clothes. Then he puts on a hair net and a painter's dust mask. He turns off all fans, air-conditioning, and such, and he washes his hands up to his elbows in warm soapy water. Next he sprays Lysol in the air and on the work surfaces and waits a few minutes before beginning. When he's ready to inoculate, he takes the tape off the jars one hole at a time just before he inoculates it being careful not to touch the lid anywhere near the holes, and he flames the needle between each jar. He tells me his contamination rate is about one jar in 80 using this technique.

Maybe it will work for you too.

Good luck!

-Diploid


--------------------
Republican Values:

1) You can't get married to your spouse who is the same sex as you.
2) You can't have an abortion no matter how much you don't want a child.
3) You can't have a certain plant in your possession or you'll get locked up with a rapist and a murderer.

4) We need a smaller, less-intrusive government.

Extras: Filter Print Post Top
InvisibleFloydian
veteran
Registered: 05/13/00
Posts: 1,022
Re: what more must I do?! [Re: Diploid]
    #1420101 - 03/31/03 03:54 PM (21 years, 2 days ago)

Thanks for the suggestions guys. I figured it was just cause I wasnt careful enough during the innoculation.

One more question. Soaking the jars and lids in bleach diluted in water for a couple hours (after throwing out nasty contaminated medium of course) should be plenty to have them ready to go again, right?


--------------------
Don't squeeze the pancake batter

Extras: Filter Print Post Top
Offlineshirley knott
not my real name
 User Gallery
Registered: 11/11/02
Posts: 9,105
Loc: London
Last seen: 7 years, 2 months
Trusted Cultivator
Re: what more must I do?! [Re: Floydian]
    #1420112 - 03/31/03 04:03 PM (21 years, 2 days ago)

right.


--------------------
buh

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   Myyco.com Golden Teacher Liquid Culture For Sale


Similar ThreadsPosterViewsRepliesLast post
* Lysol....Not Made for Disinfecting Air? motamanM 2,213 19 06/27/06 12:56 PM
by coda
* Is it contaminated ? Sacred 1,253 8 04/19/02 12:32 PM
by Sacred
* Turn Your Bathroom Into A Sterile Clean Room. knellcast 2,536 1 10/16/02 11:34 PM
by zeta
* Possible contamination questions... first timer! kspoker 1,027 6 12/16/02 02:44 AM
by Psychoslut
* Vermiculite Alternatives (Replacements)? awakeningosiris 4,022 5 02/09/03 09:03 AM
by SubGen1us
* Can Lysol be substituted? fatal_eclipse 2,631 12 08/16/04 01:43 PM
by frankz00
* Re: best vermiculite-free substrate? keyzar 1,099 2 06/05/00 02:36 AM
by brainnoise
* Post deleted by users_request malibu76 1,008 14 06/06/02 05:04 PM
by Statini

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
488 topic views. 24 members, 145 guests and 77 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.009 seconds on 14 queries.