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Flowing
Monkey

Registered: 08/16/09
Posts: 323
Last seen: 6 years, 11 months
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Grain LC, potential problem.
#14201000 - 03/28/11 10:51 PM (12 years, 10 months ago) |
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So a couple of months ago I made a GLC with Ling zhi. I aspirated it into a 20cc syringe but didn't shoot it into the distilled water. I put this in the fridge and left it for a while before inoculating jars, these jars didn't grow anything so i assume the mycelium died (senescence?) or got contaminated in the syringe from all the nutrients.
A couple of days ago, I took two 60 cc syringes of the concentrated mycelium water from my grain jars, one in a 500ml jar of distilled H2O, and about 5 cc's each into 5 litre mason jars. This was the concentrated mycelium water but it had no time to rest before it was aspirated and inoculated into the grain jars. There are still no signs of growth in these jars and it's been 3 days.
Is this blatantly wrong for some reason? I assumed that a vigorous species like P. cubensis would have no problem with this technique. Should I try again with the distilled H2O and mycelium LC?
Thank you.
-------------------- He believed that educated people could make up their own minds. His motto, as head of one of the first and most important review panels, was great encouragement: "We're not here to play God." -DMT: The Spirit Molecule
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SWIM Jr
SWIM SWAM

Registered: 02/14/11
Posts: 131
Loc: In The Pool
Last seen: 11 years, 2 months
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Re: Grain LC, potential problem. [Re: Flowing]
#14201180 - 03/28/11 11:17 PM (12 years, 10 months ago) |
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My experience is that it could take up to a week to see growth with grain LC on faster species. Maybe I shook them too much, but I thought that they were dead after 3 days, then when I checked on them in one week I saw many small colonies.
And, Ling Chi is the slowest that I have ever cultivated.
-------------------- I am mighty tighty whitey, and I am smuggling grapes.
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SWIM Jr
SWIM SWAM

Registered: 02/14/11
Posts: 131
Loc: In The Pool
Last seen: 11 years, 2 months
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Re: Grain LC, potential problem. [Re: SWIM Jr]
#14201263 - 03/28/11 11:28 PM (12 years, 10 months ago) |
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I have used grain LC to make honey/malt extract agar LC, and they growth was visible in a few days.
-------------------- I am mighty tighty whitey, and I am smuggling grapes.
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Primal Call
Earth Mage



Registered: 09/05/10
Posts: 2,766
Loc: Here until here
Last seen: 4 years, 3 months
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Re: Grain LC, potential problem. [Re: Flowing]
#14201626 - 03/29/11 12:47 AM (12 years, 10 months ago) |
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I just did my 1st grain LC last week. I thought it would take off the next day or maybe 3 at the latest, but it took about a week. I still think this is slow for an LC, but everything seems to take longer in my cold house.
Of note, the original media was rye, and the grain LC went to WBS. I'm not sure if this would slow it down, but I wouldn't be surprised if it contributed to the delayed take-off.
so on the part of your waiting 3 days, wait longer.
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theblob
Stranger

Registered: 10/29/05
Posts: 26
Last seen: 10 years, 10 months
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Re: Grain LC, potential problem. [Re: Flowing]
#14201669 - 03/29/11 01:01 AM (12 years, 10 months ago) |
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I recently did a lc transfer. poured distilled water into a colonized wbs jar (jar was 1/4 full) and then used the myc water to inoculate 7 qt jars wbs. no sign of growth after 3 days, then bam, 30% colonization in 5 days. Just wait a bit and see what happens. If technique was sterile then you should be good to go
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k00laid
NEMO


Registered: 05/03/10
Posts: 19,636
Last seen: 6 months, 5 days
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Re: Grain LC, potential problem. [Re: Flowing]
#14201847 - 03/29/11 01:44 AM (12 years, 10 months ago) |
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Quote:
Flowing said: I aspirated it into a 20cc syringe I put this in the fridge and left it for a while before inoculating jars, these jars didn't grow anything so i assume the mycelium died
There are still no signs of growth in these jars and it's been 3 days.
Quote:
3 days is nothing. keep waiting.
how long did u wait for your first batch of jars. i'd be willing to bet they would have started growing eventually.
-------------------- AMU - AMU Q & A - MyVideo Teks!
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Flowing
Monkey

Registered: 08/16/09
Posts: 323
Last seen: 6 years, 11 months
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Re: Grain LC, potential problem. [Re: k00laid]
#14203440 - 03/29/11 12:12 PM (12 years, 9 months ago) |
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Quote:
k00laid said:
Quote:
Flowing said: I aspirated it into a 20cc syringe I put this in the fridge and left it for a while before inoculating jars, these jars didn't grow anything so i assume the mycelium died
There are still no signs of growth in these jars and it's been 3 days.
Quote:
3 days is nothing. keep waiting.
how long did u wait for your first batch of jars. i'd be willing to bet they would have started growing eventually.
It's been months, they did not grow. I will likely use the jars for another species now.
-------------------- He believed that educated people could make up their own minds. His motto, as head of one of the first and most important review panels, was great encouragement: "We're not here to play God." -DMT: The Spirit Molecule
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