|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
dizzyease
1--2--3--4--5


Registered: 07/27/10
Posts: 541
Loc: Canada
Last seen: 10 years, 10 months
|
damn needle fell off of syringe twice when I was in glovebox :(
#14198023 - 03/28/11 01:49 PM (12 years, 10 months ago) |
|
|
i have a 35cc spore syringe for 10 half pint jars, and the needle (not luer lock) fell off of the needle, I brushed the plastic end with a flame, and put it back on. how likely is this to contam my jars? i havent had this happen before, i was in a glovebox btw, it fell onto a jar lid that was just pc'd, thanks everyone/
|
TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
|
Re: damn needle fell off of syringe twice when I was in glovebox :( [Re: dizzyease]
#14198127 - 03/28/11 02:08 PM (12 years, 10 months ago) |
|
|
I'm sure you'll be fine. I've had a needle come off outside of my GB & still didn't get contams from it & the open part was facing upwards.
One thing to remember is that no spore print is sterile. Therefore, if you have an MS syringe, it's not sterile either. There's always going to be a little bit of contams on them/in the syringe. We just give the mushrooms the upper hand w/ sterile procedures & sterilization.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
|
dizzyease
1--2--3--4--5


Registered: 07/27/10
Posts: 541
Loc: Canada
Last seen: 10 years, 10 months
|
Re: damn needle fell off of syringe twice when I was in glovebox :( [Re: TranscendingLife]
#14198380 - 03/28/11 03:10 PM (12 years, 10 months ago) |
|
|
alright, that makes me feel better Thanks a lot! ill just leave it for a week, then check.
|
virus1824
Mr Mushroom



Registered: 09/25/05
Posts: 1,751
Loc: Europe
|
Re: damn needle fell off of syringe twice when I was in glovebox :( [Re: dizzyease]
#14198752 - 03/28/11 04:36 PM (12 years, 10 months ago) |
|
|
had it happen a couple of times. Did not notice increase in contamination myself. I've got a small cap of pure alcohol next to me so i dip the base of the needle and the tip of the syringe in and connect it back together, and proceed as normal.
-------------------- A weekend wasted is never a wasted weekend
Edited by virus1824 (03/28/11 05:12 PM)
|
Cynosure
allow me to be your guide.


Registered: 10/06/09
Posts: 4,228
Last seen: 11 months, 12 days
|
Re: damn needle fell off of syringe twice when I was in glovebox :( [Re: virus1824]
#14198844 - 03/28/11 04:57 PM (12 years, 10 months ago) |
|
|

I melted one off by accident near the end of one of my inoculation runs. I used the flame to heat the plastic to stick it back on for my last jar. It came out just fine!
-------------------- "You can peel it [language] off the ceiling and make it dance in front of you" - McKenna <3 .
|
dizzyease
1--2--3--4--5


Registered: 07/27/10
Posts: 541
Loc: Canada
Last seen: 10 years, 10 months
|
Re: damn needle fell off of syringe twice when I was in glovebox :( [Re: Cynosure]
#14199192 - 03/28/11 06:12 PM (12 years, 10 months ago) |
|
|
im hoping for the best!
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 724 topic views. 25 members, 211 guests and 62 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|