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Offlinedizzyease
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Registered: 07/27/10
Posts: 541
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damn needle fell off of syringe twice when I was in glovebox :(
    #14198023 - 03/28/11 01:49 PM (12 years, 10 months ago)

i have a 35cc spore syringe for 10 half pint jars, and the needle (not luer lock) fell off of the needle, I brushed the plastic end with a flame, and put it back on. how likely is this to contam my jars? i havent had this happen before, i was in a glovebox btw, it fell onto a jar lid that was just pc'd, thanks everyone/


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InvisibleTranscendingLife
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Registered: 06/09/10
Posts: 21,627
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Re: damn needle fell off of syringe twice when I was in glovebox :( [Re: dizzyease]
    #14198127 - 03/28/11 02:08 PM (12 years, 10 months ago)

I'm sure you'll be fine. I've had a needle come off outside of my GB & still didn't get contams from it & the open part was facing upwards.

One thing to remember is that no spore print is sterile. Therefore, if you have an MS syringe, it's not sterile either. There's always going to be a little bit of contams on them/in the syringe. We just give the mushrooms the upper hand w/ sterile procedures & sterilization.


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Offlinedizzyease
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Registered: 07/27/10
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Re: damn needle fell off of syringe twice when I was in glovebox :( [Re: TranscendingLife]
    #14198380 - 03/28/11 03:10 PM (12 years, 10 months ago)

alright, that makes me feel better :laugh: Thanks a lot! ill just leave it for a week, then check.


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Invisiblevirus1824
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Re: damn needle fell off of syringe twice when I was in glovebox :( [Re: dizzyease]
    #14198752 - 03/28/11 04:36 PM (12 years, 10 months ago)

had it happen a couple of times. Did not notice increase in contamination myself. I've got a small cap of pure alcohol next to me so i dip the base of the needle and the tip of the syringe in and connect it back together, and proceed as normal.


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Edited by virus1824 (03/28/11 05:12 PM)


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OfflineCynosure
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Registered: 10/06/09
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Last seen: 11 months, 12 days
Re: damn needle fell off of syringe twice when I was in glovebox :( [Re: virus1824]
    #14198844 - 03/28/11 04:57 PM (12 years, 10 months ago)

:lol:

I melted one off by accident near the end of one of my inoculation runs.  I used the flame to heat the plastic to stick it back on for my last jar.  It came out just fine! :thumbup:


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Offlinedizzyease
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Registered: 07/27/10
Posts: 541
Loc: Canada Flag
Last seen: 10 years, 10 months
Re: damn needle fell off of syringe twice when I was in glovebox :( [Re: Cynosure]
    #14199192 - 03/28/11 06:12 PM (12 years, 10 months ago)

im hoping for the best! :smile:


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