Home | Community | Message Board

Avalon Magic Plants
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Bulk Substrate   Myyco.com Isolated Cubensis Liquid Culture For Sale   Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Substrate Bags

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlineroaddog
turnip
Male User Gallery

Registered: 09/29/09
Posts: 2,288
Loc: third rock from the sun Flag
Last seen: 3 years, 10 months
is this an isolate?
    #14196972 - 03/28/11 09:52 AM (12 years, 11 months ago)

Howdy folks. I guess I have a few questions on these plates. Let me begin by giving some background. I have recently been trying to find a viable way for long term storage of genetics. I figured what better way to do this, than to first come up with an isolate, with alot of rhizo growth. So I first took some PE spores, and inoculated grain. I grew that grain out, and spawned it to horse manure. Then I fruited out the sub, and took a rather large fruit, in a cluster,and cloned it to agar. So at this point, I have done 2 transfers on agar.My understanding of how to determine, if I have achieved an isolate or not, would depend solely on the growth not sectoring. I have made 9 dishes up, from one master plate. I tried to pick the sector, from the master plate,with the most rhizo growth.Also these plates are 9 days old. here are my questions.

1) If all 9 dishes are from the same sector, why dont they grow the same? why am I seeing sectors on some dishes, but none on others?

2)why am I not getting alot of rhizo growth, If the sector I took from, was very rhizo? Could it be that they are still a little too young?

3) do any of these dishes appear to be an isolate?

4) dose my strategy for attaining an isolate, seem correct?




http://

Extras: Filter Print Post Top
Invisiblebiologys
Mycologist in Trainning
Male


Folding@home Statistics
Registered: 12/21/09
Posts: 4,622
Re: is this an isolate? [Re: roaddog]
    #14196981 - 03/28/11 09:54 AM (12 years, 11 months ago)

cnt tell anything from those pics bro..

Extras: Filter Print Post Top
Offlineroaddog
turnip
Male User Gallery

Registered: 09/29/09
Posts: 2,288
Loc: third rock from the sun Flag
Last seen: 3 years, 10 months
Re: is this an isolate? [Re: roaddog]
    #14196986 - 03/28/11 09:54 AM (12 years, 11 months ago)

also here is a picture of the master dishhttp://

Extras: Filter Print Post Top
Offlineroaddog
turnip
Male User Gallery

Registered: 09/29/09
Posts: 2,288
Loc: third rock from the sun Flag
Last seen: 3 years, 10 months
Re: is this an isolate? [Re: biologys]
    #14196998 - 03/28/11 09:57 AM (12 years, 11 months ago)

Quote:

biologys said:
cnt tell anything from those pics bro..



Ya I know, they are a little rough, but its the humidity on the lid of the dishes.I wish they wouldnt do that,but I can see a few of them pretty good. see dish 4 and 8.

Edited by roaddog (03/28/11 10:03 AM)

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Bulk Substrate   Myyco.com Isolated Cubensis Liquid Culture For Sale   Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Substrate Bags


Similar ThreadsPosterViewsRepliesLast post
* clone vs. going all the way and isolating your clone Manna Inspired 4,445 15 12/11/07 08:09 PM
by Wronguy
* multispore inocculation or agar isolation?
( 1 2 all )
Peterthinks 5,453 22 02/18/08 04:43 PM
by Peterthinks
* Strain Isolation from Syringe: check my procedure.
( 1 2 all )
Omnicracker 3,757 23 07/28/06 10:56 PM
by Roadkill
* My first project: Sub Strain Isolation. Looking for critique on my tek Cubeculture 3,915 13 12/08/06 04:36 PM
by Cubeculture
* How do you cross strains, isolate genetics/traits, and how do you stabalize hightimesreader 5,717 14 09/07/08 05:38 AM
by InTheRainySeason
* differences betwen strain isolation by transfers and by cloning?
( 1 2 3 4 all )
FGL 14,980 62 10/06/09 08:13 PM
by feelfunny
* Do you isolate strains? Liquidkick 4,157 9 07/21/06 10:14 AM
by RogerRabbit
* whens the best time to isolate the strain on agar? A_Phlux 676 4 11/18/06 08:06 PM
by RogerRabbit

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
328 topic views. 35 members, 228 guests and 22 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.009 seconds on 14 queries.