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InvisibleDoc_T
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Is that an isolate?
    #14196478 - 03/28/11 06:58 AM (12 years, 10 months ago)

What do you think? Do you see sectors, or is it an isolate?

Is this an isolate?
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STAL
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OfflineRogerRabbitM
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Re: Is that an isolate? [Re: Doc_T]
    #14196572 - 03/28/11 07:37 AM (12 years, 10 months ago)

I don't see any, but take pictures of the other side too to make sure.
RR


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InvisibleDoc_T
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Re: Is that an isolate? [Re: RogerRabbit]
    #14196583 - 03/28/11 07:39 AM (12 years, 10 months ago)

Pics from the other side didn't come out well, but to the eye it looks about the same.

Thanks for the input! :thumbup:

Edit- oh, I just noticed a contam spot!
It was a sloppy pour, I'm lucky to have got what I did. Transfers have been made.


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Offlinejimmyjame1
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Re: Is that an isolate? [Re: Doc_T]
    #14196594 - 03/28/11 07:42 AM (12 years, 10 months ago)

how many transferes was that?

i really wanna invest in some agar. ive tried doing the same concept on a 1 inch layer of brf in a jar. i was able to get growth, but they got really fluffy instead of ryzo. sorta like the fuzziness on the stems of mushrooms lacking either humidity or fae (im not sure which)


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InvisibleDoc_T
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Re: Is that an isolate? [Re: jimmyjame1]
    #14196650 - 03/28/11 08:10 AM (12 years, 10 months ago)

Five or six transfers away from a tissue clone. Probably about two dozen plates total to get that.
Buy agar locally, and you can use PF jars as dishes. Short term and small scale, anyway.


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Offlinejimmyjame1
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Re: Is that an isolate? [Re: Doc_T]
    #14196664 - 03/28/11 08:14 AM (12 years, 10 months ago)

when making the transfers, should i construct a glove box. or can i do this in open air if i clean a small room and bleach the hell out of it. Asian groceries usually have this stuff right. is it labled as agar or does it have another name.


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Re: Is that an isolate? [Re: jimmyjame1]
    #14196668 - 03/28/11 08:17 AM (12 years, 10 months ago)

With a surgical facemask and gloves, you could probably get away with open air inoculation.
But, make and use a glovebox for better results.

Agar flakes, agar powder, agar agar. Available from asian markets and health food stores. Pronounced "auger-auger".


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Re: Is that an isolate? [Re: Doc_T]
    #14196675 - 03/28/11 08:19 AM (12 years, 10 months ago)

thanks doc. im gonna take a look at some places today. :smile:

stay groovy


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Invisibleanonjon
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Re: Is that an isolate? [Re: jimmyjame1]
    #14196899 - 03/28/11 09:26 AM (12 years, 10 months ago)



It's Fancy! :wink:


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Edited by anonjon (03/28/11 09:27 AM)


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Invisibleandymc
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Re: Is that an isolate? [Re: Doc_T]
    #14196968 - 03/28/11 09:51 AM (12 years, 10 months ago)

Quote:

Doc_T said:
Edit- oh, I just noticed a contam spot!
It was a sloppy pour, I'm lucky to have got what I did. Transfers have been made.




Shoulda had 'bacteria' as an additional poll option :wink:

I don't really see any sectors there, but neither do they look as uniform as I'd like them to be before slanting.  I reckon I'd do one more transfer into 3 individual dishes and let them grow out a bit to be more confident.


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Re: Is that an isolate? [Re: andymc]
    #14199147 - 03/28/11 06:04 PM (12 years, 10 months ago)

Interesting voting pattern.


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Offlinemabachko
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Re: Is that an isolate? [Re: Doc_T]
    #14199199 - 03/28/11 06:13 PM (12 years, 10 months ago)

Doc T I don't have a clue but I'm wondering about the piece above your thumb at say 7 oclock. Why is that hairly while the other areas are coarse?


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Re: Is that an isolate? [Re: mabachko]
    #14199216 - 03/28/11 06:16 PM (12 years, 10 months ago)

It's condensation and/or the plastic bag making it look fuzzy.


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Offlinemabachko
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Re: Is that an isolate? [Re: Doc_T]
    #14199228 - 03/28/11 06:18 PM (12 years, 10 months ago)

OK I'm not anywhere near this level, but information to store for use later on. Thnx


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InvisibleM11
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Re: Is that an isolate? [Re: mabachko]
    #14199256 - 03/28/11 06:22 PM (12 years, 10 months ago)

I don't see any sectors, but they sure look rhizomorphic. 

Did you just do that last transfer because of the contam, or for reassurance on whether it is an isolate or not?


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Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter.  Just pretend it's christmas.
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Those mushrooms are fine.  Your friend is a pussy.
-RR

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InvisibleDoc_T
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Re: Is that an isolate? [Re: mabachko]
    #14199276 - 03/28/11 06:26 PM (12 years, 10 months ago)

You're closer than you think. Go get some agar and get ready. :wink:

Final transfer to confirm no more sectoring and then those will be my working jars.
This one is in the fridge right now, I'll get it out tomorrow and remove the whole slice where the bad spot is.


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Offlinemabachko
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Re: Is that an isolate? [Re: M11]
    #14199279 - 03/28/11 06:26 PM (12 years, 10 months ago)

As this looks like it is not partitioned, what will happen when all 3 mingle with each other??


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InvisibleM11
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Re: Is that an isolate? [Re: mabachko]
    #14199282 - 03/28/11 06:27 PM (12 years, 10 months ago)

Doc_T, how often do you transfer?


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Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter.  Just pretend it's christmas.
-RR

Those mushrooms are fine.  Your friend is a pussy.
-RR

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InvisibleDoc_T
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Re: Is that an isolate? [Re: M11]
    #14199292 - 03/28/11 06:31 PM (12 years, 10 months ago)

Quote:

mabachko said:
As this looks like it is not partitioned, what will happen when all 3 mingle with each other??




If it's an isolate it won't matter. And it'll be in the fridge anyway, not growing.


Quote:

M11 said:
Doc_T, how often do you transfer?




Once a week or so? As needed, I guess.
Whenever the myc gets about an inch across or when I see a sector I like.

I incubate agar dishes at 78-80F btw.
And when I do agar->grain transfers, those jars go in the incubator at least until the first shake.


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