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OfflineOneU

Registered: 03/19/11
Posts: 763
Last seen: 11 years, 11 months
No glove box...
    #14195906 - 03/28/11 01:30 AM (12 years, 10 months ago)

So I had an option at a glove box but my roommates insist it is better used for storing clothes than keeping a sterile inoculation space. I disagree but it is his. I don't have any money left over to buy one. Thrift store is too far to bike or take a bus. I am left with one option. Use the small bathroom space for it.

Could I completely sterilize it (spray a lot of Lysol disinfectant spray) and go in there, wait a bit with the pot and everything I need (5 mins?) until everything settles, close all airflow and inoculate the jars there? I understand this was old method used but it really leaved me no choice. I could just disobey and use but how much does it raise my chances of contamination? Is the glove box REALLY important?

Also: could I keep the FC in a room that has no airflow other than the occasional opening of the room? I will still open the lid to fan/mist/fan but is that enough? I'm not sure how much fresh air they need. The room is 300 sq ft.

I searched and only one thread came up if I need one and it was deleted :frown:

Thank you in advance!


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Offlinek00laid
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Re: No glove box... [Re: OneU]
    #14195954 - 03/28/11 01:50 AM (12 years, 10 months ago)

use injector ports and u can shoot spores in open air.

most silicone works


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InvisibleTheShroomJew23
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Re: No glove box... [Re: OneU]
    #14195960 - 03/28/11 01:51 AM (12 years, 10 months ago)

I used to do inoculations in my bedroom. I just killed all ventilation and lysol'd the crap out of the room. I also flamed and alcohol-ed my needle in between each jar.


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OfflineOneU

Registered: 03/19/11
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Re: No glove box... [Re: OneU]
    #14196022 - 03/28/11 02:11 AM (12 years, 10 months ago)

Ok I think I will do that. I don't have money for silicon or I would just buy a glove box. Thrift store is closer than appliance store which is a shame because most everything other than a grocery store is far from me. I live up on a hilltop so biking back with a huge package (or biking up 7-8 miles up hill after riding downhill to get silicone) would be SUCH a pain. I might consider, though, since mushrooms do enjoy a bit of energetic dedication.


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OfflineJoolz
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Re: No glove box... [Re: OneU]
    #14196262 - 03/28/11 03:58 AM (12 years, 10 months ago)

Put holes in the lids of your jars, and put electric tape over the holes. Then sterilize. Once done, let them cool a day in the PC for a day or so. Take lysol, lamp, lighter, and everything else you will need (bring a towel with a wet end as well so that you can cool your syringe) and put them into a walk in closet. Spray lysol pretty heavily, wait 5 minutes, do it again, wait 5 minutes, then go in. Pull your shirt up over your mouth and nose and continue to spray lysol after every 5 minutes or so.

Take syringes out, heat ends, cool them with towel, then just poke them through the tape and into the holes, inoculate, make sure all the water drips out (wait a second before you move on) and rinse and repeat. Re-sterilize your needle after every jar assuming you didn't use all of the solution. Once done, quickly move the jars to your inoculation area and spray that heavily with lysol. Assuming its a drawer or something that will stay shut, you don't have to spray it with lysol too much. I generally do though because I will leave the tape on those holes until I see good sized patches of myc growing. I then prepare the tape (make it easy to rip off) and setup tin foil pieces and quickly swap out the tape for foil as you will need air exchange later on.

If you put verm on top of your jars (which everyone should) then it will be more than enough to keep germs out of those very small holes in the tape, especially after a good spray from the lysol can once a day for about a week. This is my little made up tech for inoculating PF jars, and I haven't had any problems so far.


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Offlinejimmy8
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Re: No glove box... [Re: Joolz]
    #14196266 - 03/28/11 04:05 AM (12 years, 10 months ago)

Are you suggesting he should flame the tip of the needle, and then wipes it down with a towel?

The first drop or so of spore solution is enough to cool the syringe and allow the rest of the solution to flow just fine. Touching the end of the needle with pretty much anything after flaming it is asking for contamination.

I'm not saying your technique doesn't work. Just saying that step is unnecessary.

P.S http://www.shroomery.org/11402/Fast-easy-fool-proof-inoculation


Edited by jimmy8 (03/28/11 04:12 AM)


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InvisibleDoc_T
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Re: No glove box... [Re: jimmy8]
    #14196331 - 03/28/11 04:56 AM (12 years, 10 months ago)

You don't need to shoot the jars in a glovebox, just be careful.

Flame the needle, expel a drop, shoot. Do not wipe the needle.


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OfflineTrippy_Smurf
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Re: No glove box... [Re: jimmy8]
    #14196350 - 03/28/11 05:07 AM (12 years, 10 months ago)

Unfortunately, I can't find it right now, but there's a tek on here somewhere that tells you how to make a GB/SAB from a cardboard box and saran wrap.


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InvisibleDynGBreeD
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Registered: 01/15/11
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Re: No glove box... [Re: Doc_T]
    #14196442 - 03/28/11 06:38 AM (12 years, 10 months ago)

Quote:

Doc_T said:
You don't need to shoot the jars in a glovebox, just be careful.

Flame the needle, expel a drop, shoot. Do not wipe the needle.



Very true, I was lazy and dint use a glove box and so for so good. But I wouldn't advise it, I did a noob move.


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Offlinetotal
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Re: No glove box... [Re: Trippy_Smurf]
    #14196444 - 03/28/11 06:42 AM (12 years, 10 months ago)

if you must do "clean work" and feel glovebox is needed, but unavailable, work inside of clear trashbags...
Lay them flat on a table and slide your stuff inside them and work...(dont try to fill them with bad air)
be sure to scrub/sanitize arms and hands as best as possible...


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InvisibleDynGBreeD
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Re: No glove box... [Re: total]
    #14196453 - 03/28/11 06:46 AM (12 years, 10 months ago)

Quote:

total said:
if you must do "clean work" and feel glovebox is needed, but unavailable, work inside of clear trashbags...
Lay them flat on a table and slide your stuff inside them and work...(dont try to fill them with bad air)
be sure to scrub/sanitize arms and hands as best as possible...



Wow, thats a head ache waiting to happen... and how do you flame your needle inside the bag without opening it and letting contams in? Wouldn't you only trap contams in still air, I would think that would be worse. Sorry im not saying your wrong, just looking for closure to become better at Mycology :pipesmoke:


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InvisibleDoc_T
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Re: No glove box... [Re: DynGBreeD]
    #14196469 - 03/28/11 06:53 AM (12 years, 10 months ago)

No flame inside glove box or bag, ever.
Still air means the contams settle out.
I've used a bag, it works ok.
Another method is to tip a tote or aquarium over on a counter, with enough hanging over the edge that you can reach up inside.
Add gloves, move carefully, and it's a still air box that's made from an unmodified container.

(Not my invention, I think I got the idea from Blutjaeger?)


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Offlinetotal
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Re: No glove box... [Re: DynGBreeD]
    #14196470 - 03/28/11 06:55 AM (12 years, 10 months ago)

If your flaming needles, you dont need to be working in a glovebox...
And to be honest, ive never flamed a needle inside a glovebox, simply flamed and moved in from the outside...

If i can pour petri's inside of one, you can do basically anything else...
If you must red hot a needle, wrap it in a alcohol soaked towel-let prior to moving in...

Working in still air is better than moving air, unless your 100% positive your air is clean...
Thats why i work infront of a 4x2 flowhood :super:

When it comes down to it, the trash bag will be cleaner than any environment your trying to work in open air...


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InvisibleDynGBreeD
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Re: No glove box... [Re: total]
    #14196477 - 03/28/11 06:58 AM (12 years, 10 months ago)

:awesomenod: Ok, thanks guys. Lol sorry OneU I wasnt trying to steal your post. Good luck bro!


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Offlinetotal
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Re: No glove box... [Re: Doc_T]
    #14196485 - 03/28/11 07:03 AM (12 years, 10 months ago)

Quote:

Doc_T said:
Another method is to tip a tote or aquarium over on a counter, with enough hanging over the edge that you can reach up inside.
Add gloves, move carefully, and it's a still air box that's made from an unmodified container.
(Not my invention, I think I got the idea from Blutjaeger?)



:highfive:

Great shit...:bongload:
Bet it works great...

Here are some pics of my old still air box...


I will rep a tupperware with holes in it for anything sterile!


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OfflineOneU

Registered: 03/19/11
Posts: 763
Last seen: 11 years, 11 months
Re: No glove box... [Re: total]
    #14200483 - 03/28/11 09:23 PM (12 years, 10 months ago)

Ok well I see these methods. I think I may be able to borrow someone's car and head to the appliance store. Probably won't do silicon because I have micropore tape and filter discs that I got because someone on shroomery told me sfd are useful (I didn't know it's only useful for bulk/grain).

My main problem is I want to get the most out of it that I can. I have a 17"x9" storage bin. I have a plexy glass the size I need for the lid too. The problem that arises is that I want to inoculate 7-8 jars so if any by the mistakes I make get contaminated I can still get a good amount out of it. If they ALL turn out well, then I don't have enough room for it, it'll overcrowd and suffocate.

This is where I'm at right now:

-12 pint jars, tapered and wide mouth
-humidifier and heater that can constantly keep the room at 73-75 degrees F.
-Distilled water
-BRF/Verm/Perlite
-Micro pore tape
-SFD
-Pot for sterilization and dunk


THINKING I should switch the plastic bin I got for a bigger one and while I'm at it stop by walmart which is next door to Lowe's and get a glove box which would be like ~$10. I have a few spare bucks since I returned a fan I had and a heat lamp which failed.

So my last question is, do I sterilize, inoculate, then put micro pore tape on? Or is it easier/better to get silicon and do that method and skip the micro pore? (Keep in mind I HAVE micro pore but would have to buy silicon)

Much appreciate thus far. Shroomery members, thank you. <3


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Offlinemill
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Re: No glove box... [Re: OneU]
    #14201746 - 03/29/11 01:18 AM (12 years, 10 months ago)

Depends of your lid tek.
I simply sterilize, innoc through the filter and then quickly tape with micropore. All is made in open air, i just sanitize the table before working in my kitchen.
If your innoc point is just a hole covered by the foil you use for sterilizing, i suggest you to tape with micropore before inoculating and then add a second layer. Never tried but dont think the micropore will resist the sterilisation.
Dont be so worry about the clean process for inoculating. But be as clean as possible as you play with G2G or agar.


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OfflineOneU

Registered: 03/19/11
Posts: 763
Last seen: 11 years, 11 months
Re: No glove box... [Re: mill]
    #14201797 - 03/29/11 01:30 AM (12 years, 10 months ago)

Quote:

mill said:
Depends of your lid tek.
I simply sterilize, innoc through the filter and then quickly tape with micropore. All is made in open air, i just sanitize the table before working in my kitchen.
If your innoc point is just a hole covered by the foil you use for sterilizing, i suggest you to tape with micropore before inoculating and then add a second layer. Never tried but dont think the micropore will resist the sterilisation.
Dont be so worry about the clean process for inoculating. But be as clean as possible as you play with G2G or agar.



Doesn't the freshly sterilized needle melt the filter? I'm not that worried about it, I just don't want to be over/under doing it. I have 12 pint jars. I just opened the box.. they aren't that small. I ordered before I see pftek. I also am getting the syringe in a few days, I need to be ready.


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Offlinek00laid
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Re: No glove box... [Re: OneU]
    #14201815 - 03/29/11 01:34 AM (12 years, 10 months ago)

Quote:

OneU said:

Doesn't the freshly sterilized needle melt the filter?




expel one drop of spore solution to cool the needle.


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Offlinemill
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Re: No glove box... [Re: OneU]
    #14201817 - 03/29/11 01:34 AM (12 years, 10 months ago)

I dunno, i never flame sterilize my needles :laugh:
I swap the needle every 12 jars. As a jar is inoculated i carefully re-cap the needle. Of course if by mistake the needle touch something else than the sterilize jar or its cap, i swap it.
For you first time i highly suggest you to sterilize it and no, i dont think it will melt the filter or at least just a hole of the size of your needle. So nothing to worry :wink:


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OfflineOneU

Registered: 03/19/11
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Re: No glove box... [Re: mill]
    #14201822 - 03/29/11 01:36 AM (12 years, 10 months ago)

omg thank you guys so much such fast and useful replies. i love this community..


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Offlinegofudgeyourself
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Re: No glove box... [Re: OneU]
    #14202827 - 03/29/11 09:30 AM (12 years, 9 months ago)

open air inoculation is fine if youre VERY careful, pf jars can be pretty forgiving but thats no reason to be negligent.

Quote:

Also: could I keep the FC in a room that has no airflow other than the occasional opening of the room? I will still open the lid to fan/mist/fan but is that enough? I'm not sure how much fresh air they need. The room is 300 sq ft.




no airflow is fine as long as you fan a couple times a day. steady airflow could actually be bad and cause your cakes to dry out faster than normal.

people imagine fanning being a pain in the ass but honestly who doesn't think staring at fruiting cakes in a terrarium is awesome?


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"turn all the hate in the world, into a mocking bird. make it fly away" :peace:


Edited by gofudgeyourself (03/29/11 09:30 AM)


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