Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Capsules   Mushroom-Hut Grow Bags   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag   Kraken Kratom Red Vein Kratom   PhytoExtractum Buy Bali Kratom Powder

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlineremykwp
Stranger

Registered: 03/14/11
Posts: 31
Last seen: 4 years, 24 days
Glove box + Air purifier. Any advantages?
    #14120693 - 03/14/11 05:44 PM (12 years, 11 months ago)

If prior to any Inoculating I stuck an air purifier in my still air box  for an hour with polyfill in the arm holes, would there be any advantages?

I have tried making spawn 5 times and they all turn green.
Any help would be great.

Extras: Filter Print Post Top
OfflineChronicSmoke
wanderer
Male

Registered: 02/25/11
Posts: 538
Loc: On the Moon
Last seen: 12 years, 8 months
Re: Glove box + Air purifier. Any advantages? [Re: remykwp]
    #14120725 - 03/14/11 05:50 PM (12 years, 11 months ago)

oust the shit out of your glovebox.  otherwise im fairley new but i sprayed mine down with oust and then i had two papertowles that were soaked in rubbing alcohol in their once everything was shut i wipe it all down again.


--------------------
This is a public computer, 1,000's of people use it everyday this isn't me typing this.  I dont even know how I got on this site, how the hell do I even work this computer.:wow: 


Extras: Filter Print Post Top
Offlinei GrOw StUFF
Stranger
 User Gallery
Registered: 01/14/11
Posts: 1,739
Loc: Space
Last seen: 9 years, 5 months
Re: Glove box + Air purifier. Any advantages? [Re: ChronicSmoke]
    #14120871 - 03/14/11 06:15 PM (12 years, 11 months ago)

Well, when you unplug the arm holes then you are back to square 1 right?....Just wash the box with alcohol and then you can spray the walls and stuff with soap water, keep it wet when you use it....What are you doing, inoculating grain jars?  Due to your failure rate there must be something wrong with your syringes or sterilization process.....Walk us through those steps.  I inoculate my jars in open air using an alcohol towel and don't have problems....


--------------------
The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.

Extras: Filter Print Post Top
Offlineremykwp
Stranger

Registered: 03/14/11
Posts: 31
Last seen: 4 years, 24 days
Re: Glove box + Air purifier. Any advantages? [Re: remykwp]
    #14120913 - 03/14/11 06:23 PM (12 years, 11 months ago)

Thanks for the reply.
I have sprayed oust and thoroughly cleaned with bleach/ alcohol.

Lysoled the whole room im working in.

Step one make grain jars. (soak for 36 hours load in quart jars with a tyvek lid and silicone injector pc for 90 min)

Step 2 inoculate jars. ( Flame sterilize needle with a blow torch cooling with an alcohol prep pad inside glove box) Note. Only 4 of the 5 times have I used a glove box. :P

Step 3 Wait. (Put jars in large tub with lid cocked to side in dark closet) Note. Tub is just used for ease of handling

Edited by remykwp (03/14/11 06:24 PM)

Extras: Filter Print Post Top
Offlinei GrOw StUFF
Stranger
 User Gallery
Registered: 01/14/11
Posts: 1,739
Loc: Space
Last seen: 9 years, 5 months
Re: Glove box + Air purifier. Any advantages? [Re: remykwp]
    #14121048 - 03/14/11 06:43 PM (12 years, 11 months ago)

WHat was your successful jar %?  You waited until the PC was to 15 psi before you started your timer right? Where di you get your syringes?  How long into the colonization process did it start to show contams?  How big are the holes in your lids?


--------------------
The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.

Edited by i GrOw StUFF (03/14/11 06:44 PM)

Extras: Filter Print Post Top
Invisiblefngbronco
Monkey Man
Male


Registered: 09/26/10
Posts: 2,877
Re: Glove box + Air purifier. Any advantages? [Re: i GrOw StUFF]
    #14121104 - 03/14/11 06:54 PM (12 years, 11 months ago)

Try soaking for only 24 hours tops, so as not to ferment the grains. Also the tyvek lid is no good I've found with past use, I get lots of bacteria when I use them. Maybe try using a metal lid over the tyvek with a 1/4"-1/2" hole in the center and another hole off to the side with silicone for inoculation. A third thing, rather than touching your sterilized needled, squirt a tiny amount of fluid through it. That cools it immediately and you can 'noc from there. Other than that the closet may be no bueno. Sounds like your glovebox may be alright.

30 minutes after I spray the glovebox I put all my stuff in and spray again, then I wait another 10 minutes minimum. I've used hospital grade cleaners and those take time as well. Gotta let the chemicals work their magic.

Good luck!


--------------------
I challenge you to challenge yourself more! When you feel complacent and ready to hang it up, challenge yourself to get over it! If you fail, don't look at it as you didn't succeed, look at it as you would a rock face you're trying to climb. Stand back, wayyyy back, and look at it and plot another path. If you can't find one, shuffle down the way a little, a little change of scenery or a view from a different angle may give you the insight you need.

Anything I state is relayed information from a friend of a friend and should be viewed as completely fictitious. I do not partake in any illegal or grey-area-of-the-law activities, but do have lots of friends who may or may not.    -fngbronco

Pill Divider Agar Tek

Extras: Filter Print Post Top
Offlinei GrOw StUFF
Stranger
 User Gallery
Registered: 01/14/11
Posts: 1,739
Loc: Space
Last seen: 9 years, 5 months
Re: Glove box + Air purifier. Any advantages? [Re: fngbronco]
    #14121140 - 03/14/11 07:01 PM (12 years, 11 months ago)

Yeah you don't want to soak for more than 24 hours because of the endospore reproduction rate.  What kind of tyvek are you using?  The post office shit sucks.


--------------------
The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.

Extras: Filter Print Post Top
Offlineremykwp
Stranger

Registered: 03/14/11
Posts: 31
Last seen: 4 years, 24 days
Re: Glove box + Air purifier. Any advantages? [Re: fngbronco]
    #14129262 - 03/16/11 06:20 AM (12 years, 11 months ago)

Yeah I've been using the post office stuff.
I got the syringe from sporeworks.
I got a b  syringe and a pf syringe, I had 1 successful jar from the b+ . 1 success from about 60 jars and 1 bag.

I "Did" wait for the pc to hit 15 psi.
The very first time I tried it was in a rye bag I bought from a shroomery vendor, I used the pf and got green :frown:

FNGBronco, Is it possible to elaborate a bit on the lid concept?
If theres any sure to win lids I'm willing.




Quote:

fngbronco said:
Also the tyvek lid is no good I've found with past use, I get lots of bacteria when I use them.




I have had no bacteria yet (not visible anyway) just green mold.

And The first 3 attempts the grain was soaked 24-26 hours, So im thinking that may not be the problem (or only problem)

I have ran out of spores now so im going to have to wait a couple weeks to try the any new ideas. Damn things are expensive!
The holes in my quart jars are 8 big 1/4 inch holes and 4 tiny 1/16 inch holes in my pints

Anyhow, Thanks for the help so far.

As much information possible would be appreciated.

By the way, I have put probably 200-300 hours into reading this forum.

Extras: Filter Print Post Top
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: Glove box + Air purifier. Any advantages? [Re: remykwp]
    #14129265 - 03/16/11 06:21 AM (12 years, 11 months ago)

Get some agar for $1.69. Grow a pure culture on agar, transfer to grains. :thumbup:


--------------------
You make it all possible. Doesn't it feel good?

Extras: Filter Print Post Top
Invisiblehamloaf
Pork Block
Male User Gallery


Registered: 12/23/09
Posts: 20,575
Loc: Oklahoma. Flag
Trusted Cultivator
Re: Glove box + Air purifier. Any advantages? [Re: remykwp]
    #14129303 - 03/16/11 06:43 AM (12 years, 11 months ago)

You want still air in a "still-air box" especially when transferring healthy mycelium away from contaminates. 


Don't wipe a syringe off with an alcohol pad when it's red hot.





The idea of a still-air box is to keep the air as still as possible while doing sterile procedure work.


Invader spores travel on dust particles through out the air so you wanna wet the inside of your still-air box with your solution immediately prior to beginning work.


Spraying your solution immediately prior to beginning will trap dust and invader spores in the solution, bring them to the floor, trapped inside of the solution until it evaporates.


Move steady and swiftly once you'r inside of your box, doing your sterile procedures.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.

Extras: Filter Print Post Top
Offlineremykwp
Stranger

Registered: 03/14/11
Posts: 31
Last seen: 4 years, 24 days
Re: Glove box + Air purifier. Any advantages? [Re: hamloaf]
    #14129492 - 03/16/11 08:17 AM (12 years, 11 months ago)

Ill post results in a month or 2 gota stack up some loot for more spores.
Wife would kill me, just bought 22 qt pressure cooker, Glove box, Jars, birdseed and a torch. :P

Thanks for help, I hope the agar method is better.

Extras: Filter Print Post Top
Invisiblefngbronco
Monkey Man
Male


Registered: 09/26/10
Posts: 2,877
Re: Glove box + Air purifier. Any advantages? [Re: remykwp]
    #14129960 - 03/16/11 10:29 AM (12 years, 11 months ago)

Another thing I like to do is pressure cook my jars and leave them alone for a couple days. Watch them for any growth.

With the hole in the lid, it's a stolen idea from Let's Grow Mushrooms DVD but RR uses filter discs. Put your lids on jars and tap a hole into it as a pilot. Get a 1/2 bit or so amd drill that hole out, then put in a smaller hole somewhere else on the lid. Put RTV on the smaller hole and let it cure. Stretch some tyvek (doubled up if it's postal stuff) over the jar opening and put the lid over that and tighten it down. The larger hole will let ge happen and keep Contaminants down(ish) nothing beats filter discs, but you still need something to prevent moisture loss.

I think the big thing tho is not touching the needle after flame sterilized, and get the whole needle, not just tip. I've let the needles go back to their normal color and put the cover back over them and sit for a little. The cover makes no contact with the needle but rather snaps onto the base.

Best of luck!


--------------------
I challenge you to challenge yourself more! When you feel complacent and ready to hang it up, challenge yourself to get over it! If you fail, don't look at it as you didn't succeed, look at it as you would a rock face you're trying to climb. Stand back, wayyyy back, and look at it and plot another path. If you can't find one, shuffle down the way a little, a little change of scenery or a view from a different angle may give you the insight you need.

Anything I state is relayed information from a friend of a friend and should be viewed as completely fictitious. I do not partake in any illegal or grey-area-of-the-law activities, but do have lots of friends who may or may not.    -fngbronco

Pill Divider Agar Tek

Extras: Filter Print Post Top
Offlineremykwp
Stranger

Registered: 03/14/11
Posts: 31
Last seen: 4 years, 24 days
Re: Glove box + Air purifier. Any advantages? [Re: fngbronco]
    #14158246 - 03/21/11 07:56 AM (12 years, 11 months ago)

BTW, not to be off topic but just to let anyone know I have some trich spores for trade :P

Edited by remykwp (03/21/11 07:57 AM)

Extras: Filter Print Post Top
Offlineremykwp
Stranger

Registered: 03/14/11
Posts: 31
Last seen: 4 years, 24 days
Re: Glove box + Air purifier. Any advantages? [Re: remykwp]
    #14195801 - 03/28/11 12:58 AM (12 years, 11 months ago)

So I ordered 5 syringes from lil shop of spores.
I got it from lil shop to make sure sporeworks syringe was not my problem. Just narrowing down any possible problems.
When they get here I'll post anything I can about the technique used. Im going to make a grain lc in a quart jar and only fill it about an inch from the bottom with wbs.
So that would leave enough air in there for it to grow without a filter. So it will be sealed completely. I just bought a brand new 22 qt pressure cooker. So the only way for contamination to get in is while knocking up. or the slight chance there is a contamination in the syringe.
But I'll do 5 different jars with 5 different syringes. I don't see how this could fail.I bleach cleaned my glovebox with a scotch brite and filled the holes with polyfill. I will be wearing rubber gloves with 90% rubbing alcohol on them that go all the way up to my elbows. I will be using a torch for my needle. and I will be wearing a face mask and hairnet(I dont see how the hair would get in the glove box anyway).
I will be knocking up 2cc each jar.

B+
Creeper
Puerto Rico
Tasmania
Texas

Extras: Filter Print Post Top
OfflineSuperPuma
Automation Nut
 User Gallery


Registered: 11/06/07
Posts: 97
Last seen: 5 years, 8 months
Re: Glove box + Air purifier. Any advantages? [Re: remykwp]
    #14195868 - 03/28/11 01:18 AM (12 years, 11 months ago)

Sounds like a high failure rate, too soon.

I don't pre-soak my rye, just cook for 45 mins then PC for 60 mins @15 psi. I've done about forty of these in the past month and I've not seen a single spawn jar go bad yet.

My glovebox is a simple tupperware with arm holes fitted with long arm rubber gloves, airtight-ish. I put my jars inside and I lysol the inside of the glovebox from the inside and get going. No flames, no alcohol, just quick, deliberate work.

First time I used this "too simple" technique I assumed either the unventilated lysol would kill everything in the glovebox or that my contam rate would be extreme from my lack of sterilization between visits to the mother spawn jar or LC. Everything went swimmingly and I've used this ever since.

I used to rock a DIY hepa filtered positive pressure glovebox, would would flame sterilize everything, soak in alcohol, etc... I guess you have to overkill before you can kiss.

I'd say check your pressure cooking hardware/technique.

Extras: Filter Print Post Top
Offlineremykwp
Stranger

Registered: 03/14/11
Posts: 31
Last seen: 4 years, 24 days
Re: Glove box + Air purifier. Any advantages? [Re: SuperPuma]
    #14262013 - 04/09/11 07:11 AM (12 years, 10 months ago)

So I got spores from a different vendor.
I filled jars 1/4 of the way full with no filter just a inoc hole with silicone, They are almost done, 1 was done but I used it for the grain lc method. So its narrowed down to the filter or the spores.
I told sporeworks I might have had a problem, so they fixed the "possible" problem :wink: one of the jars I forgot the inoc hole and heated a flathead screwdriver up till it was red hot and punched a hole in it. no green mold on that, but it did get cobweb :P.

im going to try some filter lid jars next week with the new spores ill leave feedback :smile:

Edited by remykwp (07/04/12 05:08 AM)

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Capsules   Mushroom-Hut Grow Bags   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag   Kraken Kratom Red Vein Kratom   PhytoExtractum Buy Bali Kratom Powder


Similar ThreadsPosterViewsRepliesLast post
* Flow hood/Glove box Suntzu 2,182 1 02/08/04 05:54 AM
by DieCommie
* Glove box/Lysol/Innoculation question GnuBobo 4,313 13 06/24/04 08:05 PM
by KaptKid
* Glove Box question DMJ 2,728 17 10/19/03 12:01 AM
by Hanky
* Glove Box Question The_Mushroom_God 4,322 6 04/30/02 10:51 PM
by bluepoo
* Glove box MirrorImage 3,802 19 04/28/05 06:49 PM
by doomsdayrobert
* SCARY glove box..... MilkVein 1,222 8 09/03/03 09:21 AM
by Semilanceata
* Hows this for a glove box? TheDoc 1,018 7 09/25/03 11:22 AM
by tak
* Re: Instructions for my dirt cheap glove box tom 2,911 5 04/27/01 11:54 AM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,329 topic views. 26 members, 182 guests and 50 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.005 seconds on 12 queries.