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OfflineFrequentFryer
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Registered: 03/10/11
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Spore Syringes
    #14192836 - 03/27/11 03:15 PM (12 years, 10 months ago)

So, the spores in my syringes clump up after being stored for awhile. Whats the best way to break them up? My last two jars i sucked unsterile air in them and shook them up. which i now realize is a no-no. Then again my jars dont have an contams yet..
Whats the best way to get sterile air in my syringe?
Also, if i dont use all of the spore solution in my syringe should i take the needle off and cap it, or just cap the needle?
Also you think that syringe is gonna be fine or is it not even worth using anymore?
Thanks:syringe:


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InvisibleEarth Child


Registered: 12/16/10
Posts: 3,473
Re: Spore Syringes [Re: FrequentFryer]
    #14192856 - 03/27/11 03:20 PM (12 years, 10 months ago)

I just bought 3 syringes, and my PF Classic was all clumped up.


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Offlineshroomanxiety
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Re: Spore Syringes [Re: FrequentFryer]
    #14192868 - 03/27/11 03:22 PM (12 years, 10 months ago)

u can hold the tip of the syringe in a flame and slowly draw air in. then shake until clumps break up.


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OfflineFrequentFryer
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Re: Spore Syringes [Re: shroomanxiety]
    #14192881 - 03/27/11 03:24 PM (12 years, 10 months ago)

Quote:

shroomanxiety said:
u can hold the tip of the syringe in a flame and slowly draw air in. then shake until clumps break up.



Alright this is what i was thinking. What about storage after use anyone? Cap or capped needle?
And is my syringe still good after having unsterile air in it?


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Offlineshroomanxiety
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Re: Spore Syringes [Re: FrequentFryer]
    #14192936 - 03/27/11 03:36 PM (12 years, 10 months ago)

its hard to say if the unsterile air contaimed the syringe, u can test it on petri dishes or a test batch of pf jars. you will just have to watch for contams and hope for the best.


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OfflineFrequentFryer
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Re: Spore Syringes [Re: shroomanxiety]
    #14193063 - 03/27/11 04:00 PM (12 years, 10 months ago)

Quote:

shroomanxiety said:
its hard to say if the unsterile air contaimed the syringe, u can test it on petri dishes or a test batch of pf jars. you will just have to watch for contams and hope for the best.



Alright, i got all of the unsterile air out and used a alcohol flame to draw some air in/resterilized the needle with some alcohol and put the needle cap on. I store them needle side up so they shouldn't leak. If it comes down to it i can always use a cap from my half pint cake to make a print.. It'd just kill me to throw 9 cc's of dark soution away.
Anyways, we'll see.
Thanks. :smile:


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Offlinemindenvelope
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Registered: 02/28/11
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Re: Spore Syringes [Re: FrequentFryer]
    #14193105 - 03/27/11 04:06 PM (12 years, 10 months ago)

I pulled in unsterile air into my syringe and I'm at 99% colonization with 0 contams in BRF jars.


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Re: Spore Syringes [Re: mindenvelope]
    #14193125 - 03/27/11 04:11 PM (12 years, 10 months ago)

Quote:

mindenvelope said:
I pulled in unsterile air into my syringe and I'm at 99% colonization with 0 contams in BRF jars.



Right on man. I incoc'd my first jar wednesday and now its sunday and there are major patches of growth. Im stoked. :tongue2:


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