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Invisibletiara
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longevity and calorie restriction in animals, possible application with preserving strains?
    #14191830 - 03/27/11 11:59 AM (12 years, 10 months ago)

In animals, restricting calories while providing optimum nutrition is a proven way of extending lifespan as well as good health. CRON Calorie Restriction Optimum Nutrition seems to work at least in part by triggering survival mode cellular activity. The individual organism scavenges cellular debris for energy, metabolic activity seems to slow a bit while becoming more efficient and waste products are reduced as there is less 'junk food' to be metabolized.

While storing spores or fungal tissue in sterile water or low oxygen environments, to my newbie understanding, triggers dormancy, would growing a second generation or later clone in some media that provides all micronutrients, macronutrients but little energy food produce a clone with reduced signs of senescence? Could abundant food needed for fast colonization trigger epigenetic changes that weaken the clone for the next generation? The clone would have no need to have a lean mean fighting machine metabolism to compete with another strain for food and likely survival. Could too much of a good thing be part of what triggers senescence?

I've been reading some posts where varying the growing media, type of grain, straw, manure, coir, peat, source of trace minerals may help keep a clone strain productive for more generations than repeatedly using the same formula. Variety might help trigger various epigentic metabolic factors to keep adapting to the new nutrient absorption or simply make sure that the micronutrients are adequate. Could running at an energy source level low enough to trigger high efficiency while providing critical nutrients keep a generation's vitality good enough to delay senescence?

regards,
tiara


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OfflinePrimalSoup
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Re: longevity and calorie restriction in animals, possible application with preserving strains? [Re: tiara]
    #14197093 - 03/28/11 10:26 AM (12 years, 10 months ago)

Yeah, but I don't think it's "dormancy" exactly.  As you reduce nutrition/oxygen/temperature the mycelial metabolism just slows down and eventually comes to almost a complete stop.  Reintroducing nutrition it starts again.

The variation in substrate idea seems more of a theory to me, I've heard about it for years but never found it made much difference in practice.  RR has said he uses the same (FP MEA) agar media all the time and never has issues that way.  When I run out of MEA I cook up PDY media and it always has small variations - I can see adaptation from the mycelia going on when I transfer to a new batch.  That doesn't happen with MEA, but it never results in an obvious kickstart to the culture... :shrug:

The one thing that does makes sense practically speaking is to keep the nutritive base on the sparse side, this encourages rhizomorphic growth habits as the culture stretches out looking for more food.

:peace:PS


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Offlineshroomie_glen
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Re: longevity and calorie restriction in animals, possible application with preserving strains? [Re: PrimalSoup]
    #14203544 - 03/29/11 12:41 PM (12 years, 9 months ago)

What he said:)

I recently made some slants with hpoo/coconut meat/honey in them (because I had no idea that slants are supposed to have as LITTTLE nutes in them as possible) And it literally took 3 weeks for a culture wedge to run like an inch and a half on the slant...... It was a pretty big duh :facepalm: on my behalf because I shoulda known that much nutrients would make it run slow as hell cus it had all it needed wherever it was already....


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Invisibletiara
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Re: longevity and calorie restriction in animals, possible application with preserving strains? [Re: shroomie_glen]
    #14204170 - 03/29/11 02:53 PM (12 years, 9 months ago)

thanks to you both,


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OfflinePrimalSoup
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Re: longevity and calorie restriction in animals, possible application with preserving strains? [Re: tiara]
    #14204301 - 03/29/11 03:16 PM (12 years, 9 months ago)

It'd be interesting to try an experiment to see if it did make any difference, though.  I think if I was curious enough about it I'd try it. :thumbup:

:peace:PS


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