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OfflineiSHACKA
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Registered: 03/01/11
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Last seen: 10 years, 11 months
Check my Agar
    #14187216 - 03/26/11 02:43 PM (12 years, 10 months ago)

After searching for Agar locally. I found some (so I hope)

Does this look legit?

Also, can someone please please direct me to a solid tek, not using pitri dishes as I have none yet.  I searched myself but all i get is people asking questions, not actual teks.



I want to follow the grocery store tek but i dont have potatoe flakes, can i do something like boil potatoe?


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Edited by iSHACKA (03/26/11 03:06 PM)


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InvisibleDoc_T
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Re: Check my Agar [Re: iSHACKA]
    #14187439 - 03/26/11 03:37 PM (12 years, 10 months ago)

Yup! (If that is sticks, you'll want to grind it I think.)

Search on Fast Fred's Media Cookbook. I use 30g malt, 20g agar to a liter of water.
Pour into pf jars, cap with a plastic lid, not quite snugged down, PC 60 mins or so.
Easy-peasy. A liter makes like 40-50 jars, i usually do 250 mL at a time.


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OfflineiSHACKA
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Re: Check my Agar [Re: Doc_T]
    #14187452 - 03/26/11 03:41 PM (12 years, 10 months ago)

Doc always so quick with the right advice, thanks.

When I search Fast Fred's Media Cookbook, i get 6 results, 5 of which are you just aswering peoples questios.

no tek, what gives?

:frown:


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InvisibleDoc_T
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Re: Check my Agar [Re: iSHACKA]
    #14187455 - 03/26/11 03:42 PM (12 years, 10 months ago)

I dunno, I should bookmark it after you find it for us. Try "cook book"?


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Invisiblecc2
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Re: Check my Agar [Re: Doc_T]
    #14187476 - 03/26/11 03:45 PM (12 years, 10 months ago)

guess that's what you're looking for http://www.shroomery.org/9393/FastFreds-Media-Cookbook


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OfflineiSHACKA
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Re: Check my Agar [Re: cc2]
    #14187508 - 03/26/11 03:51 PM (12 years, 10 months ago)

thanks,

really there has to be a simple picture tek out there.  I appreciate the help, and that link is a wealth of information.


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InvisibleDoc_T
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Re: Check my Agar [Re: iSHACKA]
    #14187533 - 03/26/11 03:55 PM (12 years, 10 months ago)

No pics needed.
Mix dry ingredients into cold water
boil
pour
PC


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OfflineiSHACKA
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Re: Check my Agar [Re: Doc_T]
    #14187560 - 03/26/11 04:00 PM (12 years, 10 months ago)

it is very simple yes.

I do not have malt extract, and in Toronto you have to order it. I do have dextrose.  a pack of beer yeast and potatoes.

when i boil them, how much potatoe in how much water,and then i just add the agar and go forward with the tek?


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OfflineiSHACKA
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Re: Check my Agar [Re: iSHACKA]
    #14187588 - 03/26/11 04:04 PM (12 years, 10 months ago)

ok so trying to use my brain.

"Dextrose Tek" Liquid Media (Corn Sugar Broth)

1 Teaspoon Powdered Dextrose (corn sugar)
75 ml water  [17]



I can only use 1 teaspoon of dex??? why would anyone bother fucking boiling potatoes lol


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InvisibleDoc_T
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Re: Check my Agar [Re: iSHACKA]
    #14187603 - 03/26/11 04:06 PM (12 years, 10 months ago)

Boil some potatoes like you would eat them (and then eat them) and use that water.
Add dextrose and yeast by weight, the recipe is in that link cc2 gave^^^.

Or use 1 teaspoon dextrose and 1/8 teaspoon yeast. Any yeast will do for our purposes.

Add dry ingredients to cooled potato water, then proceed. :thumbup:

Dextrose alone is a poor source of nutrients. Add potatoes and yeast.
And make agar, not LC.


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OfflineiSHACKA
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Re: Check my Agar [Re: Doc_T]
    #14187613 - 03/26/11 04:09 PM (12 years, 10 months ago)

Fuck man, i read everything you said and it made sense untill the very end.

And make agar, not LC


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Invisibleconformist
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Re: Check my Agar [Re: iSHACKA]
    #14188347 - 03/26/11 06:57 PM (12 years, 10 months ago)

I believe Doc_T was expressing his dislike of LC. He was just saying to add agar to the PDY and not waste your time on LC.


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OfflineiSHACKA
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Re: Check my Agar [Re: conformist]
    #14188478 - 03/26/11 07:18 PM (12 years, 10 months ago)

Ok good to know,

It was because of Doc replying in an LC thread I made that I decided I should learn about agar.


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OfflineiSHACKA
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Re: Check my Agar [Re: iSHACKA]
    #14193245 - 03/27/11 04:34 PM (12 years, 10 months ago)

Ok so in a few hours ill start playing with Agar.  This is what I have and what im going to do. Ill be using the grocery store tek as an outline.

500ml water - Jar
5gr potatoe flakes
9gr agar agar
20gr dextrose
2gr brewers yeast ( from a beer kit) im kinda weirded out by using this

So how does this all sound guys?  Pls let me know. Am I using enough water and will it be a good consistancy?


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InvisibleDoc_T
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Re: Check my Agar [Re: iSHACKA]
    #14193259 - 03/27/11 04:36 PM (12 years, 10 months ago)

You can use any kind of yeast. Bread yeast is fine.

Is that the recipe from the tek?


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Invisiblebiologys
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Re: Check my Agar [Re: Doc_T]
    #14193265 - 03/27/11 04:38 PM (12 years, 10 months ago)

grocery store agar tek is 2 tblspoons potato flakes, 1 teaspoon honey, 1/2 stick of agar, and 1 cup of water....


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OfflineiSHACKA
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Re: Check my Agar [Re: biologys]
    #14193426 - 03/27/11 05:20 PM (12 years, 10 months ago)

well ill be using the grocery store tek, in terms of my procedue, using a jar etc.

the recipe i got from Fastfreds media cookbook

Potato Dextrose Yeast (PDY)

200 g Potato, infusion from [dilute to 1L total] (im using potatoe flake)

20 g Dextrose
  2 g Yeast extract
20 g Agar 
  1 L Distilled water (dH2O)


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Re: Check my Agar [Re: iSHACKA]
    #14193452 - 03/27/11 05:27 PM (12 years, 10 months ago)

If it's in Fast Fred's cook book, it's good. :thumbup:

but that makes a liter- you want 500 mL, so cut ingredients in half.


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OfflineiSHACKA
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Re: Check my Agar [Re: Doc_T]
    #14193545 - 03/27/11 05:45 PM (12 years, 10 months ago)

ok so mixed these ingrediants cut in half, into a pot, with the required amount of water.

Im gonna heat untill it becomes very liquid, then add 2 table spoons per 250ml jar, tinfoil and PC for 30 mins.

Sound good?


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Re: Check my Agar [Re: iSHACKA]
    #14193571 - 03/27/11 05:52 PM (12 years, 10 months ago)

Half the amounts listed in the recipe with half the amount of liquid. Bring it to a boil, but don't let it foam up.

:thumbup::thumbup:


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OfflineiSHACKA
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Re: Check my Agar [Re: Doc_T]
    #14193608 - 03/27/11 06:04 PM (12 years, 10 months ago)

alright, keep you all posted.


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OfflineiSHACKA
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Re: Check my Agar [Re: iSHACKA]
    #14193939 - 03/27/11 07:13 PM (12 years, 10 months ago)

Ok so after boiling for about 15 minutes, the solution would not become clear, it maybe became a touch clearer.

I then put about 4tbl spoon into each of my 8 250mls jars.  By the time i had finished capping all the jars the liquid had already solidified quite a bit (normal?)

now it is in the PC for the next 30 minutes.


Update

Agar will cool for a few hours, spend the night in the fridge and get knocked up tomorrow morning.  What are my next steps.  None of the teks really cover what is done next. ill store at room temp and after if all goes well can i make needles, or use the agar itself directly into grain?

thanks


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Pain is just weakness leaving the body


Edited by iSHACKA (03/27/11 08:41 PM)


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OfflineiSHACKA
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Re: Check my Agar [Re: iSHACKA]
    #14196754 - 03/28/11 08:48 AM (12 years, 10 months ago)

finished knocking 4 jars up.

How does this agar look to you guys, normal color?  its very thick and wont move around if you put it upside down


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OfflineiSHACKA
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Re: Check my Agar [Re: iSHACKA]
    #14197892 - 03/28/11 01:24 PM (12 years, 10 months ago)

Anyone? The left over mixture that sat in my pan overnightwas kinda rubbery, but soft and moist?


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InvisibleDoc_T
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Re: Check my Agar [Re: iSHACKA]
    #14199154 - 03/28/11 06:05 PM (12 years, 10 months ago)

What about it?


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OfflineiSHACKA
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Re: Check my Agar [Re: Doc_T]
    #14199721 - 03/28/11 07:44 PM (12 years, 10 months ago)

is this ok

squirted MS onto agar ?ok

Agar and spores are in jar with 4 holes that have breathable tape and tinfoil held down my elastic band ?ok


Sitting at room temp ?ok


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Re: Check my Agar [Re: iSHACKA]
    #14199923 - 03/28/11 08:09 PM (12 years, 10 months ago)

MS->agar is ok. Use a tiny drop.

Tape? Ugh. But if the foil is over it, it's ok. Get some new lids when you can.

Room temp is ok. Not too cool, find a cozy spot.


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OfflineiSHACKA
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Re: Check my Agar [Re: Doc_T]
    #14199942 - 03/28/11 08:13 PM (12 years, 10 months ago)

what kind of lids? silicone?


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Re: Check my Agar [Re: iSHACKA]
    #14199965 - 03/28/11 08:17 PM (12 years, 10 months ago)

Plain is what I use, undrilled. Or grain lids with a polyfil puff, some have a silicone port also.


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