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iSHACKA
Sick boy


Registered: 03/01/11
Posts: 483
Last seen: 10 years, 11 months
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Check my Agar
#14187216 - 03/26/11 02:43 PM (12 years, 10 months ago) |
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After searching for Agar locally. I found some (so I hope)
Does this look legit?
Also, can someone please please direct me to a solid tek, not using pitri dishes as I have none yet. I searched myself but all i get is people asking questions, not actual teks.
I want to follow the grocery store tek but i dont have potatoe flakes, can i do something like boil potatoe?
-------------------- Pain is just weakness leaving the body
Edited by iSHACKA (03/26/11 03:06 PM)
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Check my Agar [Re: iSHACKA]
#14187439 - 03/26/11 03:37 PM (12 years, 10 months ago) |
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Yup! (If that is sticks, you'll want to grind it I think.)
Search on Fast Fred's Media Cookbook. I use 30g malt, 20g agar to a liter of water. Pour into pf jars, cap with a plastic lid, not quite snugged down, PC 60 mins or so. Easy-peasy. A liter makes like 40-50 jars, i usually do 250 mL at a time.
-------------------- You make it all possible. Doesn't it feel good?
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iSHACKA
Sick boy


Registered: 03/01/11
Posts: 483
Last seen: 10 years, 11 months
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Re: Check my Agar [Re: Doc_T]
#14187452 - 03/26/11 03:41 PM (12 years, 10 months ago) |
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Doc always so quick with the right advice, thanks.
When I search Fast Fred's Media Cookbook, i get 6 results, 5 of which are you just aswering peoples questios.
no tek, what gives?
-------------------- Pain is just weakness leaving the body
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Check my Agar [Re: iSHACKA]
#14187455 - 03/26/11 03:42 PM (12 years, 10 months ago) |
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I dunno, I should bookmark it after you find it for us. Try "cook book"?
-------------------- You make it all possible. Doesn't it feel good?
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cc2
Mush

Registered: 05/15/10
Posts: 2,611
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Re: Check my Agar [Re: Doc_T]
#14187476 - 03/26/11 03:45 PM (12 years, 10 months ago) |
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iSHACKA
Sick boy


Registered: 03/01/11
Posts: 483
Last seen: 10 years, 11 months
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Re: Check my Agar [Re: cc2]
#14187508 - 03/26/11 03:51 PM (12 years, 10 months ago) |
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thanks,
really there has to be a simple picture tek out there. I appreciate the help, and that link is a wealth of information.
-------------------- Pain is just weakness leaving the body
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Check my Agar [Re: iSHACKA]
#14187533 - 03/26/11 03:55 PM (12 years, 10 months ago) |
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No pics needed. Mix dry ingredients into cold water boil pour PC
-------------------- You make it all possible. Doesn't it feel good?
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iSHACKA
Sick boy


Registered: 03/01/11
Posts: 483
Last seen: 10 years, 11 months
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Re: Check my Agar [Re: Doc_T]
#14187560 - 03/26/11 04:00 PM (12 years, 10 months ago) |
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it is very simple yes.
I do not have malt extract, and in Toronto you have to order it. I do have dextrose. a pack of beer yeast and potatoes.
when i boil them, how much potatoe in how much water,and then i just add the agar and go forward with the tek?
-------------------- Pain is just weakness leaving the body
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iSHACKA
Sick boy


Registered: 03/01/11
Posts: 483
Last seen: 10 years, 11 months
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Re: Check my Agar [Re: iSHACKA]
#14187588 - 03/26/11 04:04 PM (12 years, 10 months ago) |
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ok so trying to use my brain.
"Dextrose Tek" Liquid Media (Corn Sugar Broth)
1 Teaspoon Powdered Dextrose (corn sugar) 75 ml water [17]
I can only use 1 teaspoon of dex??? why would anyone bother fucking boiling potatoes lol
-------------------- Pain is just weakness leaving the body
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Check my Agar [Re: iSHACKA]
#14187603 - 03/26/11 04:06 PM (12 years, 10 months ago) |
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Boil some potatoes like you would eat them (and then eat them) and use that water. Add dextrose and yeast by weight, the recipe is in that link cc2 gave^^^.
Or use 1 teaspoon dextrose and 1/8 teaspoon yeast. Any yeast will do for our purposes.
Add dry ingredients to cooled potato water, then proceed. 
Dextrose alone is a poor source of nutrients. Add potatoes and yeast. And make agar, not LC.
-------------------- You make it all possible. Doesn't it feel good?
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iSHACKA
Sick boy


Registered: 03/01/11
Posts: 483
Last seen: 10 years, 11 months
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Re: Check my Agar [Re: Doc_T]
#14187613 - 03/26/11 04:09 PM (12 years, 10 months ago) |
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Fuck man, i read everything you said and it made sense untill the very end.
And make agar, not LC
-------------------- Pain is just weakness leaving the body
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conformist


Registered: 07/29/10
Posts: 210
Loc: SE
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Re: Check my Agar [Re: iSHACKA]
#14188347 - 03/26/11 06:57 PM (12 years, 10 months ago) |
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I believe Doc_T was expressing his dislike of LC. He was just saying to add agar to the PDY and not waste your time on LC.
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iSHACKA
Sick boy


Registered: 03/01/11
Posts: 483
Last seen: 10 years, 11 months
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Ok good to know,
It was because of Doc replying in an LC thread I made that I decided I should learn about agar.
-------------------- Pain is just weakness leaving the body
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iSHACKA
Sick boy


Registered: 03/01/11
Posts: 483
Last seen: 10 years, 11 months
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Re: Check my Agar [Re: iSHACKA]
#14193245 - 03/27/11 04:34 PM (12 years, 10 months ago) |
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Ok so in a few hours ill start playing with Agar. This is what I have and what im going to do. Ill be using the grocery store tek as an outline.
500ml water - Jar 5gr potatoe flakes 9gr agar agar 20gr dextrose 2gr brewers yeast ( from a beer kit) im kinda weirded out by using this
So how does this all sound guys? Pls let me know. Am I using enough water and will it be a good consistancy?
-------------------- Pain is just weakness leaving the body
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Check my Agar [Re: iSHACKA]
#14193259 - 03/27/11 04:36 PM (12 years, 10 months ago) |
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You can use any kind of yeast. Bread yeast is fine.
Is that the recipe from the tek?
-------------------- You make it all possible. Doesn't it feel good?
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biologys
Mycologist in Trainning




Registered: 12/21/09
Posts: 4,622
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Re: Check my Agar [Re: Doc_T]
#14193265 - 03/27/11 04:38 PM (12 years, 10 months ago) |
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grocery store agar tek is 2 tblspoons potato flakes, 1 teaspoon honey, 1/2 stick of agar, and 1 cup of water....
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iSHACKA
Sick boy


Registered: 03/01/11
Posts: 483
Last seen: 10 years, 11 months
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Re: Check my Agar [Re: biologys]
#14193426 - 03/27/11 05:20 PM (12 years, 10 months ago) |
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well ill be using the grocery store tek, in terms of my procedue, using a jar etc.
the recipe i got from Fastfreds media cookbook
Potato Dextrose Yeast (PDY)
200 g Potato, infusion from [dilute to 1L total] (im using potatoe flake)
20 g Dextrose 2 g Yeast extract 20 g Agar 1 L Distilled water (dH2O)
-------------------- Pain is just weakness leaving the body
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Check my Agar [Re: iSHACKA]
#14193452 - 03/27/11 05:27 PM (12 years, 10 months ago) |
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If it's in Fast Fred's cook book, it's good. 
but that makes a liter- you want 500 mL, so cut ingredients in half.
-------------------- You make it all possible. Doesn't it feel good?
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iSHACKA
Sick boy


Registered: 03/01/11
Posts: 483
Last seen: 10 years, 11 months
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Re: Check my Agar [Re: Doc_T]
#14193545 - 03/27/11 05:45 PM (12 years, 10 months ago) |
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ok so mixed these ingrediants cut in half, into a pot, with the required amount of water.
Im gonna heat untill it becomes very liquid, then add 2 table spoons per 250ml jar, tinfoil and PC for 30 mins.
Sound good?
-------------------- Pain is just weakness leaving the body
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Check my Agar [Re: iSHACKA]
#14193571 - 03/27/11 05:52 PM (12 years, 10 months ago) |
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Half the amounts listed in the recipe with half the amount of liquid. Bring it to a boil, but don't let it foam up.

-------------------- You make it all possible. Doesn't it feel good?
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iSHACKA
Sick boy


Registered: 03/01/11
Posts: 483
Last seen: 10 years, 11 months
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Re: Check my Agar [Re: Doc_T]
#14193608 - 03/27/11 06:04 PM (12 years, 10 months ago) |
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alright, keep you all posted.
-------------------- Pain is just weakness leaving the body
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iSHACKA
Sick boy


Registered: 03/01/11
Posts: 483
Last seen: 10 years, 11 months
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Re: Check my Agar [Re: iSHACKA]
#14193939 - 03/27/11 07:13 PM (12 years, 10 months ago) |
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Ok so after boiling for about 15 minutes, the solution would not become clear, it maybe became a touch clearer.
I then put about 4tbl spoon into each of my 8 250mls jars. By the time i had finished capping all the jars the liquid had already solidified quite a bit (normal?)
now it is in the PC for the next 30 minutes.
Update
Agar will cool for a few hours, spend the night in the fridge and get knocked up tomorrow morning. What are my next steps. None of the teks really cover what is done next. ill store at room temp and after if all goes well can i make needles, or use the agar itself directly into grain?
thanks
-------------------- Pain is just weakness leaving the body
Edited by iSHACKA (03/27/11 08:41 PM)
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iSHACKA
Sick boy


Registered: 03/01/11
Posts: 483
Last seen: 10 years, 11 months
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Re: Check my Agar [Re: iSHACKA]
#14196754 - 03/28/11 08:48 AM (12 years, 10 months ago) |
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finished knocking 4 jars up.
How does this agar look to you guys, normal color? its very thick and wont move around if you put it upside down
-------------------- Pain is just weakness leaving the body
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iSHACKA
Sick boy


Registered: 03/01/11
Posts: 483
Last seen: 10 years, 11 months
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Re: Check my Agar [Re: iSHACKA]
#14197892 - 03/28/11 01:24 PM (12 years, 10 months ago) |
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Anyone? The left over mixture that sat in my pan overnightwas kinda rubbery, but soft and moist?
-------------------- Pain is just weakness leaving the body
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Check my Agar [Re: iSHACKA]
#14199154 - 03/28/11 06:05 PM (12 years, 10 months ago) |
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What about it?
-------------------- You make it all possible. Doesn't it feel good?
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iSHACKA
Sick boy


Registered: 03/01/11
Posts: 483
Last seen: 10 years, 11 months
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Re: Check my Agar [Re: Doc_T]
#14199721 - 03/28/11 07:44 PM (12 years, 10 months ago) |
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is this ok
squirted MS onto agar ?ok
Agar and spores are in jar with 4 holes that have breathable tape and tinfoil held down my elastic band ?ok
Sitting at room temp ?ok
-------------------- Pain is just weakness leaving the body
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Check my Agar [Re: iSHACKA]
#14199923 - 03/28/11 08:09 PM (12 years, 10 months ago) |
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MS->agar is ok. Use a tiny drop.
Tape? Ugh. But if the foil is over it, it's ok. Get some new lids when you can.
Room temp is ok. Not too cool, find a cozy spot.
-------------------- You make it all possible. Doesn't it feel good?
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iSHACKA
Sick boy


Registered: 03/01/11
Posts: 483
Last seen: 10 years, 11 months
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Re: Check my Agar [Re: Doc_T]
#14199942 - 03/28/11 08:13 PM (12 years, 10 months ago) |
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what kind of lids? silicone?
-------------------- Pain is just weakness leaving the body
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Check my Agar [Re: iSHACKA]
#14199965 - 03/28/11 08:17 PM (12 years, 10 months ago) |
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Plain is what I use, undrilled. Or grain lids with a polyfil puff, some have a silicone port also.
-------------------- You make it all possible. Doesn't it feel good?
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