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woutah
Royal A


Registered: 01/31/11
Posts: 159
Loc: Inside of a malnourished
Last seen: 12 years, 6 months
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Liquid culture and substrate to use after.
#14182887 - 03/25/11 05:52 PM (12 years, 10 months ago) |
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Hey, i'm currently waiting for a liquid culture to grow and i'm wondering it's been since Wednesday afternoon that i dumped some spores into a 500ml jar containing 100ml water and 4mg fine white sugar dissolved in it and a aluminium foil top. I didn't even use a syringe, i just scraped off some spores from my B+ print. (nothing sterilized, i'm too cool for that) and i think the water is already starting to become very slightly cloudy but it could just be my imagination because it's barely noticable.
 What substrates could i use when the lc is done?
- compost that consists of mowed grass - Horse dungs, but i'm really looking against collecting these. - rich potting soil
Will a large plastic box with handles that i went over with some tape to keep it airtight work as a monotub? because i was just thinking about dumping my whole lc into the monotub Or a mixture of some of these? Or do i really need vermiculite and rice flour?
~I know there is a large contamination change, but could anyone give me an estimate how large it actually would be? like 1/10 or more 95%~
I'm keeping the jar at room temperature, 17-20°
I know this is far from foolproof, if it fails, i'm gonna put some more efforts into it, if it succeeds, well then i think i'm gonna gather some friends and have the time of our lives with them
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virus1824
Mr Mushroom



Registered: 09/25/05
Posts: 1,751
Loc: Europe
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Re: Liquid culture and substrate to use after. [Re: woutah]
#14182906 - 03/25/11 05:56 PM (12 years, 10 months ago) |
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I'd inoculate a petri dish or a small jar first and see what happens, before you waste allot of time and resources. Like you said, this is far from ideal. Next time use honey instead of white sugar. After the LC i'd go for rye.
-------------------- A weekend wasted is never a wasted weekend
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anonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
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Re: Liquid culture and substrate to use after. [Re: woutah]
#14182912 - 03/25/11 05:57 PM (12 years, 10 months ago) |
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Barely noticeable? If it's slightly cloudy it's garbage. You said yourself it wasn't a very sterile procedure.
-------------------- The above post is fictional, hypothetical, or downright nonsensical.
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ronjohn7779
Stranger


Registered: 10/28/08
Posts: 1,373
Last seen: 12 years, 8 months
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Re: Liquid culture and substrate to use after. [Re: anonjon]
#14182937 - 03/25/11 06:01 PM (12 years, 10 months ago) |
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Your water should be crystal clear the whole time. MYC in water looks like pearly white clouds that hold together. Early in life they're just strands of goo barely visible. That agitation of these strands (stirring) is what causes this goo to grow into a viable culture.
Also simple table sugar is some of the worst stuff you can make LCs with. Buy some Karos clear syrup (the stuff with vanilla in it works fine too).
-------------------- "Lennox Lewis, I'm coming for you man. My style is impetuous. My defense is impregnable, and I'm just ferocious. I want your heart. I want to eat his children. Praise be to Allah!" An American Hero Iron Mike!
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woutah
Royal A


Registered: 01/31/11
Posts: 159
Loc: Inside of a malnourished
Last seen: 12 years, 6 months
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Re: Liquid culture and substrate to use after. [Re: virus1824]
#14182983 - 03/25/11 06:11 PM (12 years, 10 months ago) |
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Quote:
virus1824 said: I'd inoculate a petri dish or a small jar first and see what happens, before you waste allot of time and resources. Like you said, this is far from ideal. Next time use honey instead of white sugar. After the LC i'd go for rye.
No rye [unless september; then i can just pluck it off a field] ): But i just put the jar against a glass of clear water and guess what the jar its not cloudy or foggy, but just a little bit whitish, less see through. Still barely noticeable tough.
Lets say i make a small substrate in a petri dish, and i pour some lc over it, and mycelium covers it I'm good right? But won't the mycelium take way too much time covering it because i heard you can keep the jar only for 2 weeks or so. Is it best kept in light or dark? [google is so unclear about liquid cultures with me for some reason]
Also when do i know the jar is "ready"?
thanks already for the quick response
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virus1824
Mr Mushroom



Registered: 09/25/05
Posts: 1,751
Loc: Europe
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Re: Liquid culture and substrate to use after. [Re: woutah]
#14183023 - 03/25/11 06:16 PM (12 years, 10 months ago) |
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Hey Woutah,
First things first. Judging from what you are planning to do you haven't read enough. Please for you're own sake read through established methods first and stick to that. Doing otherwise usually ends in failure.
Make a petri dish (get agar from china town, search a recipe on here you like) and put a drop (not more) in the middle. Contams usually grow very fast. Green mold (Very white first, turns green later) can be especially vigorous. Mycelium takes a little bit longer but thats good, in my opinion it makes it easier to identify. If that turns out alright so no contaminations from the point of inoculation. You can be pretty sure the LC is clean, and move on from there.
-------------------- A weekend wasted is never a wasted weekend
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woutah
Royal A


Registered: 01/31/11
Posts: 159
Loc: Inside of a malnourished
Last seen: 12 years, 6 months
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Re: Liquid culture and substrate to use after. [Re: virus1824]
#14183133 - 03/25/11 06:34 PM (12 years, 10 months ago) |
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Quote:
virus1824 said: Hey Woutah,
First things first. Judging from what you are planning to do you haven't read enough. Please for you're own sake read through established methods first and stick to that. Doing otherwise usually ends in failure.
Make a petri dish (get agar from china town, search a recipe on here you like) and put a drop (not more) in the middle. Contams usually grow very fast. Green mold (Very white first, turns green later) can be especially vigorous. Mycelium takes a little bit longer but thats good, in my opinion it makes it easier to identify. If that turns out alright so no contaminations from the point of inoculation. You can be pretty sure the LC is clean, and move on from there.
No agar in my town, sadly, I've went to all the food shops and what comes close to one and the things that came the closest to agar was frozen sushi wish i had agar so i could grow my cultures on that ): I took a magnifying glass, and looked at the culture, saw tiny pieces of white dust. (and something at the bottom that looks like a bit of fine sand or dust that fell in) the reason I took sugar is because we had no honey or nothing else of that fancy syrups and I've read about people being successful with 4% sugar
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ronjohn7779
Stranger


Registered: 10/28/08
Posts: 1,373
Last seen: 12 years, 8 months
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Re: Liquid culture and substrate to use after. [Re: woutah]
#14183152 - 03/25/11 06:38 PM (12 years, 10 months ago) |
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use pop corn or wild bird seeds feed as your grains. I like rye better since it's a smaller grain. There for it has a larger total surface area for MYC to grow on. Basically, it make for more "potent" spawn.
-------------------- "Lennox Lewis, I'm coming for you man. My style is impetuous. My defense is impregnable, and I'm just ferocious. I want your heart. I want to eat his children. Praise be to Allah!" An American Hero Iron Mike!
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virus1824
Mr Mushroom



Registered: 09/25/05
Posts: 1,751
Loc: Europe
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Re: Liquid culture and substrate to use after. [Re: ronjohn7779]
#14183191 - 03/25/11 06:45 PM (12 years, 10 months ago) |
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Almost any honey will do. I don't know from what town you are but i cant imagine Brussels Gent or Antwerp not having chinese supermarkets that don't carry it. In any case you are pretty inexperienced from what i can tell. I would not judge the LC on eyeballing it! Knock up a small jar or 2 and see how that goes.
-------------------- A weekend wasted is never a wasted weekend
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woutah
Royal A


Registered: 01/31/11
Posts: 159
Loc: Inside of a malnourished
Last seen: 12 years, 6 months
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Re: Liquid culture and substrate to use after. [Re: virus1824]
#14183297 - 03/25/11 07:06 PM (12 years, 10 months ago) |
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Quote:
virus1824 said: Almost any honey will do. I don't know from what town you are but i cant imagine Brussels Gent or Antwerp not having chinese supermarkets that don't carry it. In any case you are pretty inexperienced from what i can tell. I would not judge the LC on eyeballing it! Knock up a small jar or 2 and see how that goes.
LIve somewhat outside brussels, on the countryside, problem is, 5/7 days i have classes, sunday i go the gentleman club in the afternoon to have a good cup of tea and saturday I do cleaning & shopping in the small town next to me but yeah I'm not gonna special time, only to drive 2 hours (lol traffic) to get to the chinese part of brussels only to get myself some fancy agar I would do a growlog about how you shouldn't do it but my asshole friend has "borrowed" my 500€ camera
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