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OfflineKilljoy
TheHyperdimensionalSlug

Registered: 01/28/03
Posts: 865
Last seen: 3 years, 9 months
Syringes for colonization, more cc = more mycelium!
    #1418121 - 03/30/03 12:39 PM (15 years, 2 months ago)

Here is my gophers syringe tek:

-Shotglass
-isopropyl/rubbing alcohol
-syringe
-distilled water

1. Use the alcohol to sterilize the shotglass.
2. Place fully opened cap over the top of the shotglass, place under another container (keep environment sterile), and leave overnight.
3. Assemble syringe(s) (they are sterile when bought new), and fill with distilled water (which is sterile when bought new).
4. Eat the cap, or put it in your dessicator or something, and squirt the water from the syringe(s) into the shotglass full of spores, rinsing as many off the sides as possible.
5. Mix it around and suck up as many spores as you can with the water.
6. Let the syringe sit overnight in the fridge. (only if you feel like it, I didn't last time and it still worked fine using them directly after making them.)
7. Innoculate.

But yeah, if you use 2cc's to innoculate jars, they will colonize at roughly TWICE the rate as jars innoculated with 1cc.
One can only assume the more solution you use, then more the spores spread out in the jar, and the faster they will colonize.  One print will make one DARK ass syringe, or a couple good ones, which may be preferable for immediate use.

Oh, and instead of using 4 holes punched in the lid, put 5 by adding one in the center, and inject solution all the way to the bottom as well as the top.  Speeds up colonization like a mofo.

I used double the solution to innoculate the bottom jars.
  :laugh:

That's PC'd WBS+verm+BRF btw... 


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Offlinex_bonerz_x
Du Ma May ConCac Lon!

Registered: 01/21/03
Posts: 163
Last seen: 15 years, 1 month
Re: Syringes for colonization, more cc = more mycelium! [Re: Killjoy]
    #1418133 - 03/30/03 12:51 PM (15 years, 2 months ago)

so for one jar, how many days does it take for it to fully colonize with 2cc ???


1 cc of mine fully colonized at 11 days...


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OfflineKilljoy
TheHyperdimensionalSlug

Registered: 01/28/03
Posts: 865
Last seen: 3 years, 9 months
Re: Syringes for colonization, more cc = more mycelium! [Re: x_bonerz_x]
    #1418138 - 03/30/03 12:56 PM (15 years, 2 months ago)

I'll let you know when it's fully colonized, but by the looks of it, 2cc's+5 holes will be between 5 and 7 days :smile:


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Invisiblegeorge castanzaM
Lord Of The Idiots!
I'm a teapot User Gallery Arcade Champion: BurgerTime, Space Invaders
Registered: 10/21/02
Posts: 7,549
Trusted Cultivator
Re: Syringes for colonization, more cc = more mycelium! [Re: Killjoy]
    #1418209 - 03/30/03 01:45 PM (15 years, 2 months ago)

and if your short on syringes,

kramer tells the story of how he only had 8cc of cambodian spore water left.
it was a bad situation because kramer likes the cambodians.
well he took these 30 some 1/2 pint jars(wbs and verm) with plastic lids with one hole in the middle and inoculated each jar with 1/4 cc though the hole in the middle
well let me tell you that not one jar contaminated and in about 20 days kramer had lots of cambodian mycelium to play with.


--------------------
Kramer Cakes


[url=[url=

   

:pm:


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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Vermiculite, Wild Bird Seed

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