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OfflineiSHACKA
Sick boy


Registered: 03/01/11 Happy 13th Shroomiversary!
Posts: 483
Last seen: 11 years, 26 days
Question about trich
    #14175224 - 03/24/11 11:20 AM (12 years, 11 months ago)

Hello Everyone

I recently tried making some BRF jars which fell victim to trich. Ive done my research and id say my TEK was faily solid.  So my quesiton is.

I have a plant room in my basement, filled with maybe 6-10 plants of differnet sizes that grow year round. Ive heard trich is found a lot in soil.

So basically am I screwed?  Is my basement overloaded with trich?


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Pain is just weakness leaving the body

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Offlineblue_lotus
Seventeenth Moon Rising


Registered: 01/20/11
Posts: 98
Last seen: 4 years, 3 months
Re: Question about trich [Re: iSHACKA]
    #14177554 - 03/24/11 06:03 PM (12 years, 11 months ago)

I would first invest in a couple (2 or more) of small - medium sized HEPA filter units.  Mart stores typically have them for around 30$ each.


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[18:25] < > yeah, my neighbor's cat has said the same thing before.  Only issue she's having is pinning.  She said it's taking forever....
[18:25] <-> thats cause ur neighbor is letting a cat do it
[18:26] < > cats bring something to the table, actually
[18:26] < > cat milk is a great addition to a casing, she says
[18:27] < > the only way to extract the cat milk is by pressing it's claws out and hissing at it while poking it in the rear
[18:28] < > just sit a cup underneath and it's almost automatic!

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OfflineGNB
Stranger
Registered: 03/06/08
Posts: 11
Last seen: 12 years, 9 months
Re: Question about trich [Re: blue_lotus]
    #14180814 - 03/25/11 07:59 AM (12 years, 11 months ago)

Was the contamination seen during colonization or after birthing?

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OfflineiSHACKA
Sick boy


Registered: 03/01/11 Happy 13th Shroomiversary!
Posts: 483
Last seen: 11 years, 26 days
Re: Question about trich [Re: GNB]
    #14180857 - 03/25/11 08:15 AM (12 years, 11 months ago)

During colonization, always near the top layer of dry verm.


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Pain is just weakness leaving the body

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OfflineGNB
Stranger
Registered: 03/06/08
Posts: 11
Last seen: 12 years, 9 months
Re: Question about trich [Re: iSHACKA]
    #14184213 - 03/25/11 09:33 PM (12 years, 11 months ago)

Quote:

iSHACKA said:
During colonization, always near the top layer of dry verm.




What accommodations do you have for air exchange in your lids(filter disk, Tyvek, micropore tape, etc.)?  Assuming you inoculated with a syringe, does the penetration depth correspond to the the depth at which the needle would have penetrated?

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Invisiblebiologys
Mycologist in Trainning
Male


Folding@home Statistics
Registered: 12/21/09
Posts: 4,622
Re: Question about trich [Re: GNB]
    #14185195 - 03/26/11 01:47 AM (12 years, 11 months ago)

like said above, if you're sterilizing properly then its one of two things


either your filter isn't working, or your syringe is dirty


also, are you flaming your needle between jars, do you have injection ports, or just going thru the tape or whatever..

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OfflineiSHACKA
Sick boy


Registered: 03/01/11 Happy 13th Shroomiversary!
Posts: 483
Last seen: 11 years, 26 days
Re: Question about trich [Re: biologys]
    #14185896 - 03/26/11 08:58 AM (12 years, 11 months ago)

I had no filter on my lid, just the open hole with a dry layer of vern under the lid.  I looked at my jars a lot and sometimes could hear the dry firm sliding around when i accidentily tilted my jars.

I know now this was a really bad mistake.  I probably should have covered my holes wiht microspore tape?

I did flame between every inoculation, i steamed for 90 minutes, let cool over night. Lysol's my room and did the deed.


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Pain is just weakness leaving the body

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