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Offlineshroom-jitsu
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Lion's Mane Woes
    #14174529 - 03/24/11 08:01 AM (12 years, 11 months ago)

Hey All,

Hoping your all your springtime patches are looking plentiful!

I've had a lot of success with shiitake, and they will always be a staple for me in this hobby.  Unfortunately, I haven't really had much luck with any other edibles.  The one I'm presently struggling with is the Lion's Mane.

I ordered a syringe a few weeks back from Sporeworks.  loaded up 3 LC jars, all 4% conc, 1 was honey, 1 was Karo, 1 was 50/50.  All of these LCs turned brown within days, smelled like yeast and showed no expansion of myc at all.  All cultures failed to inoculate grain. 

I directly inoculated a grain jar from this syringe, which did in fact show some rapid growth, which quickly morphed into primordia invitro and showed no further colonization.  Moisture in the grain seemed fine.  I spawned the grain to a 50/40/10 mix of Verm, Saw and BRF.  This has sat for over a month now with no sign of growth.  No odor, still smells like dough and sawdust.

I now have a second syringe.  I've searched extensively for a thorough TEK or grow log, but the ones I find are either tandem grows or just simple overviews.  Further, I can't see what anyone who has been successful has done differently.

Any "tricks-o-the-trade" anyone can share with me that are specific to Lion's Mane cultivation?  I have a fresh syringe in the fridge all ready to go!

I promise the Shroomery community that I will, without a doubt, post a very picture-heavy and detail-laden TEK in this forum (every bit as thorough as BRF TEK) if I succeed in my efforts.

Best Regards!

SJ

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Invisiblebiologys
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Re: Lion's Mane Woes [Re: shroom-jitsu]
    #14174542 - 03/24/11 08:05 AM (12 years, 11 months ago)

i've not tried this either, but from what i've read RR say, is lion's maine is well known for forming pin's early in the jars...but he also says they still colonize fine :shrug:

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Invisiblebiologys
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Re: Lion's Mane Woes [Re: biologys]
    #14174553 - 03/24/11 08:11 AM (12 years, 11 months ago)

http://www.shroomery.org/forums/showflat.php/Number/11158075#11158075

also noobie, has been a great mentor to me and this is a post by him, maybe it will help ya out
http://www.shroomery.org/forums/showflat.php/Number/9622323#9622323

Quote:

RogerRabbit said:
Different species colonize at different rates.  Lion's mane mycelium can look almost invisible, yet still be growing.  In fact, many times a lion's mane substrate that is fully colonized and fruiting will not even look like it's colonized.  Check out this one.
RR




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Offlineshroom-jitsu
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Re: Lion's Mane Woes [Re: biologys]
    #14174565 - 03/24/11 08:14 AM (12 years, 11 months ago)

Thanks for that.  I read this a few days back, which is why I still have the bag "colonizing" on my shelf.  The problem is, its still completely loose, breaks up and falls about the bag when it's handled, even gently.  No colonization for me. :frown:

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Invisiblebiologys
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Re: Lion's Mane Woes [Re: shroom-jitsu]
    #14174570 - 03/24/11 08:17 AM (12 years, 11 months ago)

:frown: im sure someone will come to help ya, wish I could..but all I really do is actives and oysters..I'm getting ready to do some PF style cakes to test out my shiitake culture for the first time and have 2 rye bags colonizing now that i'll be spawning to sawdust/bran blocks for the first time.

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Offlineshroom-jitsu
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Re: Lion's Mane Woes [Re: shroom-jitsu]
    #14174572 - 03/24/11 08:18 AM (12 years, 11 months ago)

I just found this in the threads you linked me:

http://www.shroomery.org/forums/showflat.php/Number/9622323#9622323

I can't believe I missed this one.  I know for a fact I've specifically searched for all of Noobie's logs before - he's got some awesome ones on here.

Does anyone have any others?  Obviously, despite 15 years as an IT Engineer, my Googlepathy(tm) (Goo-guh-lep-uh-thee) is far from perfect.

Edited by shroom-jitsu (03/24/11 08:18 AM)

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Invisiblebiologys
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Re: Lion's Mane Woes [Re: shroom-jitsu]
    #14174574 - 03/24/11 08:19 AM (12 years, 11 months ago)

it just linked me to the first post in his log lol so not sure what you just found :o

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Offlineshroom-jitsu
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Re: Lion's Mane Woes [Re: biologys]
    #14174591 - 03/24/11 08:25 AM (12 years, 11 months ago)

Too early for good brainwork I guess.  Thank you for the link anyway!

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Invisiblelazy bones
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Re: Lion's Mane Woes [Re: shroom-jitsu]
    #14174897 - 03/24/11 09:54 AM (12 years, 11 months ago)

Here's what I did. It's a bit lacking in details, but I think the most pertinent info is there. The block was left in a room at about 72F until it was colonized. Then I cut open the bag and put it in the greenhouse.

If you have ?'s though about more specifics I'd happily help.
:smile:

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InvisibleMonkeyKnifeFight
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Re: Lion's Mane Woes [Re: lazy bones]
    #14176849 - 03/24/11 04:16 PM (12 years, 11 months ago)

If your LC's turned brown and smelled like yeast then they were contaminated.  There is no mystery to hericium they grow like mad.  If you're using LC then you need to grow out each jar on an agar plate to test for contamination.  Otherwise you will have problems no matter what species it is. 

In the end hericium is much easier to grow than shiitake.  No weird steps necessary to initiate fruiting just grow from agar -> grain -> sawdust and it will fruit, sometimes before you want it to.  If your grain jars are stalling out then you are using contaminated LC.  There should be no dough smell.

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Re: Lion's Mane Woes [Re: MonkeyKnifeFight]
    #14177497 - 03/24/11 05:52 PM (12 years, 11 months ago)

Thanks for the reply.  You've definitely made me feel better about my current grow.  Keeping it very simple and a much closer eye on sterile tech in the culture phase.

I do understand the risks of LC, but they are so damn convienent to use!  I usually do test them first, just on a jar of grain instead of agar (expensive around here).  It reeks the same if its contamed, agar or seed.  I will definitely do this next time to be sure.  I guess I was just excited to grow a new mushie! :smile:

As far as LC in general, i must disagree out of principal (I am definitely in the minority).  I've done too many LCs to bother bullshitting you with an arbitrary number, but its a good number.  This would be the 3rd contamned LC of them all, over years.  If you follow good sterile tech, LC is perfectly safe.  It's just really good to know that a standard LC concoction should work with the manes.  I always wonder what wood lovers might like different in an LC.

My bags always smell like dough and saw - I supplement with BRF, which smells a bit like dough (to me) after cooking for an hour.  My point was that it didn't smell like contam at all.  That bag, well over a month old now, was drenched in my dirty LC.  No growth, bacteria, trich or otherwise.  No weird smells.  No colors.  Nothing. :frown:  I'm sure the LC was contamed in some way, just it apparently doesnt like sawdust too much.

Edited by shroom-jitsu (03/24/11 05:54 PM)

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Offlineshroom-jitsu
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Re: Lion's Mane Woes [Re: shroom-jitsu]
    #14209204 - 03/30/11 12:00 PM (12 years, 10 months ago)

OK, based on feedback I've received, I've decided to try the LC route again.  I inoculated 100ml of 4% honey solution 3 days ago.  Every day the mass has more then doubled.  Nothing like my 1st attempt at all.  My LC was definitely, uniformly, contaminated.  I'll never figure out how all 3 LCs contaminated, but not my grain spawn (that just dried out).

A bit more attention to detail and I have what appears to be a voracious culture.  Looking at how Lion's Manes grow, coupled for my fondness of cakes, I'm going to do my first LM grow with jars.  I plan to use toothpicks as a wood source, just as I did in the grow in my first sig link.  I plan on just fruiting straight from the tops of the open jars in my SGFC.

Does anyone see any immediate concerns with this proposal?  Are Lions Manes as sensitive to FAE and light as oysters?  Do they prefer higher CO2 during colonization like shiitake?  Are they very humidity sensitive after fruiting has started?  Any shocking necessary?

-SJ

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InvisibleMonkeyKnifeFight
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Re: Lion's Mane Woes [Re: shroom-jitsu]
    #14210455 - 03/30/11 04:27 PM (12 years, 10 months ago)

They need FAE but probably not like oysters.  I dunno I do both outside and both are happy with that.  I fruit from bags so I'm not sure what will happen with your jars.  The substrate needs humidity but if the air is too humid the fruit will get soggy and won't be happy.  They don't need a cold shock.

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Offlineshroom-jitsu
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Re: Lion's Mane Woes [Re: MonkeyKnifeFight]
    #14211971 - 03/30/11 08:58 PM (12 years, 10 months ago)

Well, I've just prepped 4 jars as a trial run. 

1/4c ground wbs
1/4c brf
1/3c verm
1 full kabob skewer, chopped up (I never use sawdust in jars)
dry verm barrier

I usually always use coffee in some form or fashion.  Since I've never pulled off a lions mane grow before, I wanted to do the first without.  That way I can better gauge the actual benefit (if any).  I'm pretty conservative with this hobby - I don't like unnecessary steps or ingredients.

If I can judge by how aggressive the culture is in liquid suspension, it will tear these jars up pretty fast.  I'll start a new thread and post the link here if it turns out to be worth sharing.

Thanks for the help!

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