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mushroom_mind_fuck
Stranger



Registered: 01/28/11
Posts: 36
Last seen: 12 years, 7 months
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PENIS ENVY WILL NOT COLONIZE
#14170993 - 03/23/11 03:57 PM (12 years, 10 months ago) |
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It has been 4 weeks since I knocked up my jars and I still see nothing at all. I injected the sides of the substrate as well...What do yall think the problem could be?
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Moisture content is probably off, meaning too dry.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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Patlal
You ask too many questions



Registered: 10/09/10
Posts: 44,797
Loc: Ottawa
Last seen: 21 hours, 6 minutes
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There is a problem with your substrate or your syringe.
Try again.
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orange771
Registered: 01/26/11
Posts: 288
Last seen: 1 year, 7 months
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Re: PENIS ENVY WILL NOT COLONIZE [Re: Patlal]
#14171137 - 03/23/11 04:21 PM (12 years, 10 months ago) |
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I'm gonna venture a guess of too wet, that was my problem and now I have 10/10 jars at about 60%
Trial and error buddy, but yeah moisture content is a major one
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mushroom_mind_fuck
Stranger



Registered: 01/28/11
Posts: 36
Last seen: 12 years, 7 months
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Re: PENIS ENVY WILL NOT COLONIZE [Re: orange771]
#14193382 - 03/27/11 05:07 PM (12 years, 10 months ago) |
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Ok thanks...I followed RR's Videos...kinda sucks
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Patlal
You ask too many questions



Registered: 10/09/10
Posts: 44,797
Loc: Ottawa
Last seen: 21 hours, 6 minutes
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Quote:
mushroom_mind_fuck said: Ok thanks...I followed RR's Videos...kinda sucks
U taking a stab at RR?
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: PENIS ENVY WILL NOT COLONIZE [Re: Patlal]
#14195142 - 03/27/11 10:42 PM (12 years, 10 months ago) |
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Quote:
Patlal said:
Quote:
mushroom_mind_fuck said: Ok thanks...I followed RR's Videos...kinda sucks
U taking a stab at RR?
Always keep in mind in this hobby that:
"What works for one, does NOT necessarily work for the other."
We all have different mycroclimates w/in which we work. You can make as uniform of an assumption as you would like, but it doesn't work like that in mycology. We all have to ADAPT to our own environment. We can read & read & read & research, then do it again. However, unless you've done it on your own, it doesn't work 100% according to someone else's plan.
We all adapt & grow in this hobby. That's what this forum is all about:
ADAPTATION
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 4 days
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Quote:
mushroom_mind_fuck said: It has been 4 weeks since I knocked up my jars and I still see nothing at all.
Bunk spores. Even if you screwed up the cakes, you'd still see at least something by now. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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mushroom_mind_fuck
Stranger



Registered: 01/28/11
Posts: 36
Last seen: 12 years, 7 months
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Re: PENIS ENVY WILL NOT COLONIZE [Re: RogerRabbit]
#14198901 - 03/28/11 05:09 PM (12 years, 10 months ago) |
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Yeah it was probably because it was left out of the refrigerator for like 4 days after it arrived. Unfortunately the damn person didn't tell me I had gotten a package until almost a week later...I got them from SporeWorks which I heard was a great vendor which I do like them for sending another syringe when one is defective but this was the 2nd PE Syringe that was defective. I told them the problem and asked for a Golden Teacher syringe instead so I hope these work..wish me luck and thanks for the responses...what is the best substrate for GT STRAIN? Also Louisiana is my location at the moment.
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M11
White Thumb

Registered: 06/14/09
Posts: 1,840
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What was the date listed on your PE syringe?
-------------------- Of course, then there's the problem of eating vermiculite. On the bright side, it makes your poop and teeth glitter. Just pretend it's christmas. -RR Those mushrooms are fine. Your friend is a pussy. -RR Outdoor Patch
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Michael Logical
Stranger

Registered: 03/10/09
Posts: 18
Last seen: 11 years, 8 days
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Re: PENIS ENVY WILL NOT COLONIZE [Re: M11]
#14283062 - 04/13/11 10:20 AM (12 years, 9 months ago) |
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Im dealing with a similar experience with a SW PE Syringe. Bought it a few months ago, maybe from the same batch. HOWEVER. I use a flowhood and have immaculate sterile technique. I got growth on Agar, WBS, LC, and even on Hpoo/Verm/WBS spawn bags. HOWEVER. it was all very weak, and very slow. no rhizomes anywhere to be seen. I am in the process of a large bulk grow to sort out a fruiting substrain from this less than impressive PE multispore. ( i had to abandon the agar work for isolation, the strain didnt take to it very well at all, considering how slow it was going.)
I would say that isolating PE into a healthy substrain should be priority if you plan on using it, it is a freak mutant from the depths of consciousness itself.
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rustycobwebs
Green mold cultivator

Registered: 12/17/05
Posts: 799
Last seen: 8 years, 9 months
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Quote:
Michael Logical said: ( i had to abandon the agar work for isolation, the strain didnt take to it very well at all, considering how slow it was going.) .
Michael, were you using the same agar recipie the whole time? I've noticed that adding a pinch of rye flour or other additives sometimes makes growth more vigorous, though im by no means an agar expert.
original poster: Besides bunk spores, it has to be your procedure. PE is very difficult to grow, took about 45 days to colonize a quart of grain for me, and i gave up trying to get it to fruit for now. Just make sure you follow all the precautions, I don't want to assume you're a noob but make sure your jars are cooled off and all of that before you noc them up. etc.
-------------------- and let it be so Praise Psilocybe
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Michael Logical
Stranger

Registered: 03/10/09
Posts: 18
Last seen: 11 years, 8 days
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Re: PENIS ENVY WILL NOT COLONIZE [Re: rustycobwebs]
#14284804 - 04/13/11 04:05 PM (12 years, 9 months ago) |
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Quote:
rustycobwebs said:
Quote:
Michael Logical said: ( i had to abandon the agar work for isolation, the strain didnt take to it very well at all, considering how slow it was going.) .
Michael, were you using the same agar recipie the whole time? I've noticed that adding a pinch of rye flour or other additives sometimes makes growth more vigorous, though im by no means an agar expert.
original poster: Besides bunk spores, it has to be your procedure. PE is very difficult to grow, took about 45 days to colonize a quart of grain for me, and i gave up trying to get it to fruit for now. Just make sure you follow all the precautions, I don't want to assume you're a noob but make sure your jars are cooled off and all of that before you noc them up. etc.
same agar i was using on my oysters and other cubes, must be the PE. Its a tough one but if you can get it to fruit is is worth all the effort.
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Michael Logical
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Registered: 03/10/09
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Last seen: 11 years, 8 days
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I should note that it often takes 3-5 agar transfers to get a nice rhizomorphic sector. i didnt make it far.
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