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Offlineiacs69
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4 days and no germination.
    #14154681 - 03/20/11 04:16 PM (12 years, 11 months ago)

I just started 5 brf jars 4 days ago.  This is my first time trying to culitvate.  I got my spores from ralphsters about a month ago, but didnt have vermiculite til last week.  It was freezing outside when I got the spores in the mail, but the liquid was not frozen when I opened it.  I kept them in a cool dark place for the entire time until inoculation, and I still see no germination.  Is this a problem or am I just being impatient?

P.S. I see/smell no signs of contamination.


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Invisiblebiologys
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Re: 4 days and no germination. [Re: iacs69]
    #14154687 - 03/20/11 04:18 PM (12 years, 11 months ago)

you're being impatient, it can take upto 2 weeks before you see first signs of growth.

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Offlinestranger_danger
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Re: 4 days and no germination. [Re: iacs69]
    #14154690 - 03/20/11 04:18 PM (12 years, 11 months ago)

4 days is no time... if u have  NO signs in ANY jars after 10 i'd start getting concerned

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Offlineagonzalez64
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Re: 4 days and no germination. [Re: iacs69]
    #14154697 - 03/20/11 04:19 PM (12 years, 11 months ago)

By germination i guess you mean "colonization". 4 days is too early to tell, give a a week or 2 to see any signs. I inoculated 10 pf tek jars about a week ago and i saw signs 7 days later. Just gonna have to waited out bro. What strain you inoculated?

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OfflineTheIndoCloud
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Re: 4 days and no germination. [Re: iacs69]
    #14154698 - 03/20/11 04:19 PM (12 years, 11 months ago)

you need to describe more. temp? tek/procedurea used? etc.


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InvisibleDoc_T
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Re: 4 days and no germination. [Re: TheIndoCloud]
    #14154753 - 03/20/11 04:32 PM (12 years, 11 months ago)

Put your jars away and don't look at them for ten full days.


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Offlineagonzalez64
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Re: 4 days and no germination. [Re: Doc_T]
    #14154807 - 03/20/11 04:45 PM (12 years, 11 months ago)

Quote:

Doc_T said:
Put your jars away and don't look at them for ten full days.




that is very hard to do. :frown: i can only go around 5 days then i give up.

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Offlineiacs69
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Re: 4 days and no germination. [Re: agonzalez64]
    #14156254 - 03/20/11 09:14 PM (12 years, 11 months ago)

The strain is A+
Right now my thermostat is set to 68 but the room I have them in is closest to the furnace so it gets considerably warmer in there.


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Offlineafrosheen
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Re: 4 days and no germination. [Re: iacs69]
    #14156272 - 03/20/11 09:16 PM (12 years, 11 months ago)

Temperature probably isn't an issue as long as it's 68F or higher. Just wait, spores are very slow sometimes.


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OfflinePrimal Call
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Re: 4 days and no germination. [Re: iacs69]
    #14156310 - 03/20/11 09:22 PM (12 years, 11 months ago)

Quote:

iacs69 said:
The strain is A+
Right now my thermostat is set to 68 but the room I have them in is closest to the furnace so it gets considerably warmer in there.




be sure they aren't over ~78F.

and yeah, 4 days is nothing for spores. patience patience patience


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Offlineiacs69
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Re: 4 days and no germination. [Re: Primal Call]
    #14157913 - 03/21/11 04:14 AM (12 years, 11 months ago)

Okay thanks guys..  Also, I'm going to be building a shotgun terrarium with perlite as a humidifier.  Only thing is that I've got miracle grow perlite with added fertalizer.  Is this an issue?  I was thinking maybe I could throw it in the bathtub with some hot water and dish soap to wash the fertalizer off, then rinse a couple of times before use.


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InvisibleDoc_T
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Re: 4 days and no germination. [Re: iacs69]
    #14158098 - 03/21/11 06:24 AM (12 years, 11 months ago)

It's fine, use it like normal. Small and expensive but won't cause troubles.


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Offlineiacs69
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Re: 4 days and no germination. [Re: Doc_T]
    #14158148 - 03/21/11 06:54 AM (12 years, 11 months ago)

I'm using a 13 watt cfl bulb for lighting.  Am I supposed to light it during colonization or am I supposed to wait until fruiting?  And also will having the lamp too close to the jars get them too hot and kill the spores?

Also I ground up my brown rice in a coffee grinder myself but it was still kind of coarse.  Kind of a sand like texture.  Might this inhibit growth at all?


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InvisibleDoc_T
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Re: 4 days and no germination. [Re: Doc_T]
    #14158155 - 03/21/11 06:55 AM (12 years, 11 months ago)

I stand by my advice from upthread:

Quote:

Doc_T said:
Put your jars away and don't look at them for ten full days.




Ok to start new jars, but stop messing with these.


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Offlineiacs69
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Re: 4 days and no germination. [Re: Doc_T]
    #14158168 - 03/21/11 07:05 AM (12 years, 11 months ago)
Log in to view attachment

So theres a pic of one of my jars.

Edit: and yah I feel yah.  I'm not really messing with them at all just want to make sure I'm doing everything right on my first grow..
So yah lighting them is fine then?

Edited by iacs69 (03/21/11 07:07 AM)

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InvisibleDoc_T
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Re: 4 days and no germination. [Re: iacs69]
    #14158174 - 03/21/11 07:09 AM (12 years, 11 months ago)

Every time you touch a jar,
tip a jar,
breathe on a jar,
handle a jar,
shed skin cells near a jar,
you risk contamination and failure.

Put your jars away, don't mess with them for ten days.


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Offlineiacs69
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Re: 4 days and no germination. [Re: Doc_T]
    #14158186 - 03/21/11 07:15 AM (12 years, 11 months ago)

Right I get it, will do.
But that still doesnt answer my question, I can just sit them away in the dark for ten days, they dont need any light?


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Offlineiacs69
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Re: 4 days and no germination. [Re: iacs69]
    #14158271 - 03/21/11 08:07 AM (12 years, 11 months ago)

nvm looked around, found my answer :]
I'll post back in 10 days or so and let you guys know how they're doing..  Until then I think I'll start a few more jars.

I read somewhere if you prepare jars and let them sit for 24 hours before steralizing, then hard to kill bacterial endospores will start to grow, thus making them easier to kill and making for a better sterilization.  And also I read it doesnt hurt to steralize twice.  What are your guys thoughts on this?


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Offlineafrosheen
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Re: 4 days and no germination. [Re: iacs69]
    #14161311 - 03/21/11 08:23 PM (12 years, 11 months ago)

Quote:

iacs69 said:
I read somewhere if you prepare jars and let them sit for 24 hours before steralizing, then hard to kill bacterial endospores will start to grow, thus making them easier to kill and making for a better sterilization.  And also I read it doesnt hurt to steralize twice.  What are your guys thoughts on this?




That applies to grains, specifically wild bird seed, and that's the 24 hour soak. Germinate the endospores then nuke them in the PC. With BRF this doesn't count, there's really nothing to soak.


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Offlineiacs69
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Re: 4 days and no germination. [Re: afrosheen]
    #14170920 - 03/23/11 03:44 PM (12 years, 11 months ago)

Well it's been 7 days now and I couldnt help but check on my jars, and in one of them I see a little spot of mycelium :]


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