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OfflineJoeker
"I know that I know nothing."


Registered: 01/01/11
Posts: 68
Loc: British Virgin Islands
Last seen: 10 years, 10 months
A good way (time) to gauge 20% colonization?
    #14169379 - 03/23/11 11:11 AM (12 years, 10 months ago)

My spawn bags have been going for just over three weeks now. You can see the timeline here, including when I noticed mycelium growth for each bag. I didn't do a very good job injecting the spore solution against the bag. I don't know why, I knew better, but I didn't. Now by eye it's going to be pretty hard for me to gauge when to massage them, so I was hoping you guys could help with a time estimate?...

I know spores can take up to two weeks to germinate and that there's a lot of variables to account for, but all I'm after is an estimate. An average time it takes your spawn bags to reach ~20% from spores. I was thinking I'd wait four weeks.


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Invisiblebiologys
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Registered: 12/21/09
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Re: A good way (time) to gauge 20% colonization? [Re: Joeker]
    #14169389 - 03/23/11 11:13 AM (12 years, 10 months ago)

if its been 3 weeks i'd go ahead and do one of them, and see just how colonized it is on the inside, if its not very much then leave the rest

if it looks like its a good amount of mycelium go ahead and do the rest.


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OfflineJoeker
"I know that I know nothing."


Registered: 01/01/11
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Loc: British Virgin Islands
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Re: A good way (time) to gauge 20% colonization? [Re: biologys]
    #14169627 - 03/23/11 12:05 PM (12 years, 10 months ago)

Thanks. Should I not let the grains touch the filter when mixing?


Edited by Joeker (03/23/11 12:10 PM)


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Offlinefaceyneck
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Re: A good way (time) to gauge 20% colonization? [Re: Joeker]
    #14170152 - 03/23/11 01:33 PM (12 years, 10 months ago)

Quote:

Joeker said:
Thanks. Should I not let the grains touch the filter when mixing?




I don't worry about it personally. Others take steps to avoid the grains touching the filter, but I don't and I also get virtually no contaminations from doing so. I've also done manure-based substrate in bags, and allowed the manure to touch the filter while mixing, and haven't noticed any problems with that. In both circumstances. The very few times I had contamination was always without question because of a faulty culture/inoculant.

...as to when to massage the bags:

I'd wait until it LOOKS like about 20%. If you think it might be 20% overall, you can massage now. If you massage once and then the growth looks splotchy again, and looks like it could use another mixing, go ahead.

I've mixed bags more than once on many occasions, including grain jars. It always works out fine. :super: I've never experienced this "stalled growth" thing in regards to over-mixing or something like that. :grin:


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OfflineJoeker
"I know that I know nothing."


Registered: 01/01/11
Posts: 68
Loc: British Virgin Islands
Last seen: 10 years, 10 months
Re: A good way (time) to gauge 20% colonization? [Re: faceyneck]
    #14170368 - 03/23/11 02:06 PM (12 years, 10 months ago)

Thanks mate, much appreciated.


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