|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
wisdom187
Stranger
Registered: 10/12/10
Posts: 301
|
is mycelium supposed to grow only ontop of agar?
#14168898 - 03/23/11 09:26 AM (12 years, 10 months ago) |
|
|
and not fully colonize it?
|
MidRange
Stranger
Registered: 01/10/11
Posts: 296
Last seen: 9 years, 3 days
|
Re: is mycelium supposed to grow only ontop of agar? [Re: wisdom187]
#14168971 - 03/23/11 09:49 AM (12 years, 10 months ago) |
|
|
Yes, and you would only want it to grow on top. This makes it easier to separate the healthy culture from any undesirables. Think of it as the game operation where all the problem spots are visible and all it takes is some careful extraction. If you where to try to do the same with any "3d" media like rye it would be like brain surgery to remove a tumor, you can spend hour poking trough it and not remove all of it.
-------------------- "Sacrifice is the Christian way of life. Theft is the Socialist way of achieving all ends. Together they represent an awesome evil for this nation to confront." Marc Emery. People who receive medical cannabis trough the federal government.
Edited by MidRange (03/23/11 09:52 AM)
|
wisdom187
Stranger
Registered: 10/12/10
Posts: 301
|
Re: is mycelium supposed to grow only ontop of agar? [Re: MidRange]
#14169351 - 03/23/11 11:06 AM (12 years, 10 months ago) |
|
|
Quote:
MidRange said: Yes, and you would only want it to grow on top. This makes it easier to separate the healthy culture from any undesirables. Think of it as the game operation where all the problem spots are visible and all it takes is some careful extraction. If you where to try to do the same with any "3d" media like rye it would be like brain surgery to remove a tumor, you can spend hour poking trough it and not remove all of it.
so is it ok to plant some non-colonized agar into the grain with the mycelium? or is it best to try to seperate the myc from the agar
|
RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 4 days
|
Re: is mycelium supposed to grow only ontop of agar? [Re: wisdom187]
#14169440 - 03/23/11 11:26 AM (12 years, 10 months ago) |
|
|
You drop the mycelium-covered agar right into the grains. Don't try to separate the mycelium from the agar. It needs the agar there to get a good jump off into the grains.
Mycelium will grow on the surface of agar, and this can be used to advantage. If you have bacteria on a plate, you can pour piping hot agar over the top of it, and when the mycelium climbs up through the new layer to reach the surface, shave it off and transfer to a new dish. This is a great way to separate mushroom mycelium from bacteria. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
|
wisdom187
Stranger
Registered: 10/12/10
Posts: 301
|
Re: is mycelium supposed to grow only ontop of agar? [Re: RogerRabbit]
#14169749 - 03/23/11 12:32 PM (12 years, 10 months ago) |
|
|
Quote:
RogerRabbit said: You drop the mycelium-covered agar right into the grains. Don't try to separate the mycelium from the agar. It needs the agar there to get a good jump off into the grains.
Mycelium will grow on the surface of agar, and this can be used to advantage. If you have bacteria on a plate, you can pour piping hot agar over the top of it, and when the mycelium climbs up through the new layer to reach the surface, shave it off and transfer to a new dish. This is a great way to separate mushroom mycelium from bacteria. RR
well before i read this i already cut the agar in half so its got like half mycelium and half agar.
are you supposed to drop it in the grain and leave it or shake the jars? i didnt know so i shook 3 and left 3
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 1,198 topic views. 21 members, 189 guests and 48 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|