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Offlinenewbiemycologist
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Registered: 03/01/11
Posts: 60
Last seen: 10 years, 7 months
You guys arent going to believe what this noob did...
    #14167523 - 03/22/11 11:16 PM (12 years, 10 months ago)

Sterilized 20 jars, had enough solution for about 13, saw spores stuck to the sides of the syringe. used distilled water and refilled syringe, flicked it around to get those bad boys floating in there and knocked up the rest. what are the odds of those jars inoculating at all??? I appreciate all feedback and constructive criticism. Thanks in advance guys.


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Offlineghotbijr
Wishes he was tripping
Male

Registered: 01/10/11
Posts: 250
Loc: Seattle, Wa, USA Flag
Last seen: 11 years, 6 months
Re: You guys arent going to believe what this noob did... [Re: newbiemycologist]
    #14167548 - 03/22/11 11:21 PM (12 years, 10 months ago)

There may not be enough spores to get them going, but I guess they're microscopic and if you could see some then maybe you'll luck out. I'd also worry about contams getting in when you refilled it. Either way it's worth the try, keep us updated.


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Offlinehighc
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Registered: 08/31/07
Posts: 3,592
Loc: maryland
Last seen: 7 years, 2 months
Re: You guys arent going to believe what this noob did... [Re: ghotbijr]
    #14167566 - 03/22/11 11:26 PM (12 years, 10 months ago)

I do it all the time.  If im running low i use like half the syringe then stick it through an unopened bottle of deer park n fill it back up. Never had a problem


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Invisiblebiologys
Mycologist in Trainning
Male


Folding@home Statistics
Registered: 12/21/09
Posts: 4,622
Re: You guys arent going to believe what this noob did... [Re: ghotbijr]
    #14167570 - 03/22/11 11:26 PM (12 years, 10 months ago)

im sure it'll have enough spores to get going, since there are millions of them in a single spore syringe, the problem is you didn't use sterilized water, so you have a good chance of contam's


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OfflineTheIndoCloud
Trying to keep on tokin.
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Registered: 09/18/10
Posts: 145
Last seen: 3 years, 7 months
Re: You guys arent going to believe what this noob did... [Re: biologys]
    #14167734 - 03/22/11 11:56 PM (12 years, 10 months ago)

Should work. I once had a syringe that I had emptied and had an extra BRF jar. So I sucked up the water inside the PC, knowing it was sterile, and vuala! it worked just like the rest. I think a lot of spores stick to the side of the syringes after being emptied. I did that for shits and giggles. Worked!


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