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OfflineCuriousGeorge83
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Colonized Rye back to Agar
    #14166238 - 03/22/11 06:12 PM (12 years, 10 months ago)

So back when I used to only do peroxidated agar (which I stopped doing) I used to knock up rye jars with multispore then wait til full colonization and transfer a piece of colonized rye to a dish of peroxidated MYA.  This would allow me to then see the sectoring of different substrains and then isolate what I saw was best rhizomorphic growth.

Would it be true then, because the mycelium was started on rye and thus now has a "taste" for grain, that when inoculating more rye jars with the agar inoculated with that myceliated rye kernel, the rye jars would take off faster because of already knowing how to grow on rye?


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InvisibleTheShroomJew23
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Re: Colonized Rye back to Agar [Re: CuriousGeorge83]
    #14166276 - 03/22/11 06:18 PM (12 years, 10 months ago)

Yes but since they are multispores still, an isolate would have much better colonization speeds. Agar has also stated however that introducing mycelium to final growth medium to start with shows no overall increase in yield or speed.


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OfflineCuriousGeorge83
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Re: Colonized Rye back to Agar [Re: TheShroomJew23]
    #14166309 - 03/22/11 06:25 PM (12 years, 10 months ago)

Thanks, I guess I meant to say after getting an isolate on agar and then transferring back to grain.  Do you think it is faster than starting the spore on agar, getting an isolate and then transferring to rye?


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OfflineThe shroomy 1
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Re: Colonized Rye back to Agar [Re: CuriousGeorge83]
    #14166363 - 03/22/11 06:37 PM (12 years, 10 months ago)

The only way you're going to get a true isolate is by starting on agar. If you do a multispore on ANY grain, then you still have to wait for the spores to "Do their thing". So you wait for it to do it on agar or you wait for a jar to fully colonize.  Doesn't make much sense.

Quote:

I guess I meant to say after getting an isolate on agar


  What Isolate?  You said that it was a multispore on grain. :shrug:

I appreciate the thought process, but if I were you, I would stick to the tried and true methods of getting your isolate by starting from agar from the get go.


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OfflineCuriousGeorge83
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Re: Colonized Rye back to Agar [Re: The shroomy 1]
    #14166395 - 03/22/11 06:42 PM (12 years, 10 months ago)

Thanks for the input. As stated before I don't do peroxidated agar anymore, so I can start on agar (which I do now). I simply was saying that I used to use the rye to germinate the spore...then when the jar was fully colonized I'd take a piece of it and put it on agar.  The mycelium would then sector out and I would isolate a sector I thought looked good.  Is this not consider strain isolation even though it's not the conventional way of doing it??


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OfflineThe shroomy 1
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Re: Colonized Rye back to Agar [Re: CuriousGeorge83]
    #14166437 - 03/22/11 06:48 PM (12 years, 10 months ago)

Ok, I get it now.  Yes, you can do it that way to begin the process of isolation.  My question now is, why are you stuck with the idea that "you don't do peroxidated agar anymore".  I'm not sure why that would matter.  If you are impeccable in your sterile process you won't have to use peroxide in your agar, (which is not recommended by any means.)


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OfflineCuriousGeorge83
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Re: Colonized Rye back to Agar [Re: The shroomy 1]
    #14166495 - 03/22/11 06:56 PM (12 years, 10 months ago)

Yep, it matters because I couldn't start spore on peroxidated agar, meaning I would start on rye.  Back then it was easier for me to not contaminate a rye jar then to not contaminate agar dishes, which is why I peroxidated them.  Now I don't so I can just start out on agar...

This is besides the point.  I guess what I was originally trying to ask was once mycelium gets used to a specific substrate (rye) does it then grow faster (in future transfers) on it because it knows and likes the substrate? Example being: spore grown out on rye, back to agar, then back to rye versus spore on agar, then to rye.  Sorry for the confusion (and hopefully this isn't still confusing and or totally stupid to even ask).


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OfflineCuriousGeorge83
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Re: Colonized Rye back to Agar [Re: CuriousGeorge83]
    #14166581 - 03/22/11 07:08 PM (12 years, 10 months ago)

And so I guess to simplify even further: does mycelium get used to certain substrates; favor them (obviously different species favor specific substrates); get sick of them; grow faster on one because it's used to it or grow slower on one because it's not used to it?


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