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OfflineiSHACKA
Sick boy


Registered: 03/01/11
Posts: 483
Last seen: 10 years, 11 months
More Trich, More questions. Need Advice
    #14163387 - 03/22/11 07:25 AM (12 years, 10 months ago)

Hello

Today I found another BRF cake infected with trich and I need advice on more then one issue at the moment. I need to make the most of the resources I have left and figure out how this happened. This may be a bit long....

So my first attempt at BRF, did all the reading, videos etc. Still managed to forget to wipe the jar before my dry layer of verm.
-Steamed jars for 90 mins
-Flamed needle every time. Plastic gloves, rubbing alcahol etc

Out of 6, so far one has trich, and two have SLOW colonization compared to others.

Made 6 more then next day, with 100% following tek. This morning one of these has tric, and possibly a second which ill post up.

Trich?

This one is confirmed trich

Are all my remaining jars likely trich?  Could it have been my procedure? I sware I followed Pf tek done right tek exactly. Could I have bought a bad needle?


Aside from this.

I had purchased another needle, with which so far I have only attempted on LC which has no signs of growth about 5 days in.

Should I attempt more LC to try and preserve what I have so I can keep working? What should I do with my remaining needle to maximize it? 

Right now I have 3 cakes that are about 50% and another 2 that are like 25%


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Pain is just weakness leaving the body


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InvisibleDoc_T
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Re: More Trich, More questions. Need Advice [Re: iSHACKA]
    #14163396 - 03/22/11 07:34 AM (12 years, 10 months ago)

NO LC!!!!

If the green is at or near the injection sites, you injected it. Bad syringe.
If it does not follow the injection pattern, or if an unshot control jar shows green, it's your procedure.
Either way, LC is the very worst way to proceed.


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You make it all possible. Doesn't it feel good?


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OfflineiSHACKA
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Re: More Trich, More questions. Need Advice [Re: Doc_T]
    #14163406 - 03/22/11 07:36 AM (12 years, 10 months ago)

Does the first Jar I posted look healthy?

What would be better then LC then?


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InvisibleDoc_T
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Re: More Trich, More questions. Need Advice [Re: iSHACKA]
    #14163410 - 03/22/11 07:38 AM (12 years, 10 months ago)

Quote:

iSHACKA said:
What would be better then LC then?




What would be better than LC from a dirty syringe? Almost anything.
But first you need to nail down your contam source.


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You make it all possible. Doesn't it feel good?


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OfflineiSHACKA
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Registered: 03/01/11
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Re: More Trich, More questions. Need Advice [Re: Doc_T]
    #14163420 - 03/22/11 07:42 AM (12 years, 10 months ago)

ok

As per your advice, the trich does appear to be near the injection sites.

Although as I stated, maybe you didnt catch it.  I have bought a totally new needle since then.  Assuming this needle is safe, which it should be.


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Offlinemexicanjewlucas
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Re: More Trich, More questions. Need Advice [Re: Doc_T]
    #14163427 - 03/22/11 07:44 AM (12 years, 10 months ago)

i don't know much but i would say the dry verm layer has something to do with it (not wiping down the jar). i steam for 30 mins and use isopropyl alcohol to wipe down the edge before adding the dry verm. never had any contam while colonizing. i also use a different needle tip for each jar (might loose a bit of what i'm putting in but i know its clean).


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"We can get 2 birds stoned at once."


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OfflineiSHACKA
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Re: More Trich, More questions. Need Advice [Re: mexicanjewlucas]
    #14163430 - 03/22/11 07:45 AM (12 years, 10 months ago)

Well I packed a pretty thick layer of dry verm. I also steamed for 90 minutes.

Can someone comment on the first photo I posted up. does that look trich, its not green yet


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Pain is just weakness leaving the body


Edited by iSHACKA (03/22/11 07:47 AM)


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Offlinemexicanjewlucas
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Re: More Trich, More questions. Need Advice [Re: iSHACKA]
    #14163435 - 03/22/11 07:47 AM (12 years, 10 months ago)

Quote:

iSHACKA said:
Still managed to forget to wipe the jar before my dry layer of verm.





--------------------
"We can get 2 birds stoned at once."


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OfflineiSHACKA
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Registered: 03/01/11
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Re: More Trich, More questions. Need Advice [Re: mexicanjewlucas]
    #14163451 - 03/22/11 07:55 AM (12 years, 10 months ago)

is the first jar trich, please i need to know im about to boil all the others.


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OfflineiSHACKA
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Re: More Trich, More questions. Need Advice [Re: iSHACKA]
    #14164236 - 03/22/11 12:02 PM (12 years, 10 months ago)

anyone?


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Invisiblehiggledy-piggledy

Registered: 08/24/10
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Re: More Trich, More questions. Need Advice [Re: iSHACKA]
    #14164248 - 03/22/11 12:03 PM (12 years, 10 months ago)

when in doubt, throw it out.


... looks suspicious to me.


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OfflineiSHACKA
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Registered: 03/01/11
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Re: More Trich, More questions. Need Advice [Re: higgledy-piggledy]
    #14164442 - 03/22/11 12:41 PM (12 years, 10 months ago)

What looks suspicious?  There is no green in that first photo.  But how does the myc look


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Pain is just weakness leaving the body


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Invisiblehiggledy-piggledy

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Re: More Trich, More questions. Need Advice [Re: iSHACKA]
    #14164519 - 03/22/11 01:01 PM (12 years, 10 months ago)

Quote:

iSHACKA said:
There is no green in that first photo.



im talking about the more greyish stuff right next to the myc.

maybe a shot from another angle will clear this up.


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OfflineiSHACKA
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Re: More Trich, More questions. Need Advice [Re: higgledy-piggledy]
    #14164579 - 03/22/11 01:14 PM (12 years, 10 months ago)

oh ok, no that is totally how it looks.  Not the angle.  Its grey and has a weird consistancy.


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Offlinei GrOw StUFF
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Re: More Trich, More questions. Need Advice [Re: iSHACKA]
    #14164647 - 03/22/11 01:29 PM (12 years, 10 months ago)

If you followed all the rules to the T and used only 1 syringe then it must be the syringe.....Did you keep the pot at a boil when you sterilized with the top on?


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The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.


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OfflineiSHACKA
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Re: More Trich, More questions. Need Advice [Re: i GrOw StUFF]
    #14165460 - 03/22/11 04:06 PM (12 years, 10 months ago)

I kept the lid on and it was definitly boiling. plus i went for a 90 minute boil.

Kinda sucks if its my syringe because that means all my cakes are probably gonna be CRAP?


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Offlinei GrOw StUFF
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Re: More Trich, More questions. Need Advice [Re: iSHACKA]
    #14165506 - 03/22/11 04:17 PM (12 years, 10 months ago)

If the syringe is bad then all the jars most likely will be bad....Time to try a different one so you can narrow down what you are doing wrong


--------------------
The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.


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Offlineshr00m3r

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Re: More Trich, More questions. Need Advice [Re: iSHACKA]
    #14165522 - 03/22/11 04:20 PM (12 years, 10 months ago)

Doesn't sound like you have got into agar work thus far but if you did want to try to salvage from the bad syringe you could try to germ some spores on agar and transfer healthy myc from any contam, Once you get a pure agar dish you can take a clean needle with clean water it in, Shoot the water into the agar dish use tip of needle to scrape of a bunch of myc tissue and suck it all up, It sounds ghetto but it works great for a FOAF who has gotten good cultures from super contaminated syringes.

IF the syringe was the cause of it, I would say all the jars are toast, If the mold starts at the top of the cake I would blame verm layer, The verm FOAF uses (im sure its no different then any other verm) shrinks when cooked so theres quite a bit of room in the barrier some times if you dont pack it good, A little curiosity and jar molestation could result in contam but it would start at top not where injection points are.


--------------------


Ban Thurian spawned straight to coir! :strokebeard2:


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OfflineiSHACKA
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Registered: 03/01/11
Posts: 483
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Re: More Trich, More questions. Need Advice [Re: shr00m3r]
    #14165549 - 03/22/11 04:25 PM (12 years, 10 months ago)

For future reference, should i pack the dry layer of verm night and tight?

I left it loose, afraid it would hinder G/E

Ill also admit i looked that these jars way to often.


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Offlineshr00m3r

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Re: More Trich, More questions. Need Advice [Re: iSHACKA]
    #14165561 - 03/22/11 04:27 PM (12 years, 10 months ago)

Pack it 100% full it wont really compress so just make sure its fully covered right up to the rim, Even packing it tight its still gonna shrink a bit, Fight the temptation to pick the jars up and move them quickly or turn them upside down to see bottom.

You wont any have problems with G.E as the verm is very porous, Even packing it tight it can still breath very well.


--------------------


Ban Thurian spawned straight to coir! :strokebeard2:


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