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notguilty
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Registered: 01/22/11
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Liquid culture question
#14160706 - 03/21/11 06:26 PM (12 years, 10 months ago) |
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I made up 6 or 7 multispore liquid cultures using honey and distilled water, of which one grew and looked healthy. It is at least a month old at this point and it still looks healthy, but each jar I've tested it on barely start to grow mushroom mycelium before it is taken over by black pin mold. I was hoping that my sterile procedure was at fault but I have reproduced the results several times.
My question is if anyone has ever had this happen, and if the two fungi could be coexisting in my beautiful liquid culture, as there is are no visible indications of contams.
The only other explanation I have is a bale of straw that got wet on the bottom, sitting in the corner of the garage. To my delight, I had been cultivating some brilliant greens and browns right next to my work bench, so I suppose I could just have lots of that particular spore floating about.
I will knock up more jars today using a different syringe for each innoc, and of course relocate my work area away from the hazmat.
Also I am now aware that shooting spores into honey water is not the proper way to make a liquid culture. I would still like to save this pint because other than one dry wbs jar colonizing at the speed of smell, I am out of spores/grain and waiting on the mailman.

Edit: The mycelium collects and recovers quickly after repeated violent shaking, and there is definately little mycelium specks here and there some other thin fuzziness starts showing black dots. I assume this is mucor/black pin mold, but this is the first time I have seen it. It took over immediately when I had incubation temperature too high, lowering the temp slowed it down considerably but the myc didnt stand a chance.
Edited by notguilty (03/21/11 06:33 PM)
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i GrOw StUFF
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Re: Liquid culture question [Re: notguilty]
#14160785 - 03/21/11 06:44 PM (12 years, 10 months ago) |
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Thats what blows about liquid culture....You have no idea what is colonizing until you try it on some jars. For better results, if you have a pressure cooker, do a grain liquid culture....
-------------------- The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.
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notguilty
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Registered: 01/22/11
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Last seen: 11 years, 9 months
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yes I will be doing grain lc's in the future
the irony of the situation is that i shot up all that honey water to make sure I didn't run out of innoculant, cause I was down to like 4cc of multispore. now i'm down to the one lc and losing hope.
to clarify, when i say that i see little white specks, i mean in popcorn/wbs test jars, briefly before the mold wins. the LC recovers quickly, not the jars. the LC is pretty old, so i thought if i was growin trich or something it would be obvious by now. can my blob be harboring mucor?
Edited by notguilty (03/21/11 07:38 PM)
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i GrOw StUFF
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Re: Liquid culture question [Re: notguilty]
#14161069 - 03/21/11 07:38 PM (12 years, 10 months ago) |
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Can't tell if the LC is good even with a nice blob in there...Can be multiple things growing in there at once...
-------------------- The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.
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notguilty
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Registered: 01/22/11
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thanks.
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dr.hax
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Re: Liquid culture question [Re: notguilty]
#14161266 - 03/21/11 08:15 PM (12 years, 10 months ago) |
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Why don't you try some agar media propagation? You could use it as a technique to isolate a healthy mycelia culture.
Good luck! :]
-------------------- Currently looking for an 'Albino' strain print. P.Equador prints for trade.
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